Objective To detect the pain condition in patients with Parkinson's disease(PD) and explore the clinical significance.Methods From May 2007 to February 2008,pain was examined in 162 PD patients in Department of Neurology,West China Hospital,Sichuan University and in 135 healthy controls well matched for gender,age and education.Statistics analysis was employed according to gender,age,the form of morbility,early-onset PD or late-onset PD,duration and Hoehn-Yahr degree.Results The incidence of pain in PD patients(53.1%)was higher than that in the healthy controls(23.7%)(P

Objective To investigate the expression o f telomerase activity in pheochromacytoma and to assess its utility as a prognos tic marker. Methods Telomerase activity was estimated in 23 samples of pheohromocytoma tissue and 6 samples of normal adrenal medullae by autoradiography-based telomeric repeat amplification protocol. The reaults w ere analized with follow-up findings. Results Of the 2 3 pheochromocytoma samples, 2 had telomerase activity and both the telomerase-p ositive pheochromocytomas apparently arose from extraadrenal tissues. All normal adrenal medullae samples were negative for telomerase activity. During follow- up, we found both of the telomerase positive patients developed mutiple metastat ic lesions after surgery. Only one of 21 telomerase negative patients clinically developed bilateral lung metastases and a local recurrence after surgery. Conclusions Telomerase activity may be used as an important i ndicator of potentially malignant pheochromocytoma and may serve as a prognosti c marker.

Research of the impacts of probation administration on teaching quality for students'clinical probation study is to control the whole process of probation teaching,which is through the strengthening of administration on three parts,including pre-clinical probation,clinical probation and post-clinical probation,so that administration elements of teaching for probation forms close links,including formulation of teaching plan goals,organization and implementation of the probation process,means and methods of probation,and evaluation of effects and quality of probation results and quality of student evaluation of teaching management trainee factors into a closed ring. Considering highly the administration process,refining the administration flow-sheet,identifying administration objectives,and systematic evaluation of the quality of teaching trainees to achieve continuous improvement of the quality of probation teaching reflect the concept of "student-centered" teaching management concepts,and provides a new approach to enhancing the quality of clinical probation teaching for adapting to the new model of medical education and management development needs.

ObjectiveTo investigate the clinical efficacy of Jin’s eye three-needle acupuncture as main therapy for acquired paralytic strabismus.MethodSeventy patients with acquired paralytic strabismus were randomly allocated to acupuncture and Western medication groups. Both groups of enrolled patients took prednisone,methycobal, vitamin B1and vitamin B12. In addition, theacupuncture group received Jin’s eye three-needle acupuncture as main therapy. The clinical therapeutic effects and adverse reactions were observed after four and eight weeks of treatment.ResultThe total efficacy rate was 75.0% in the acupuncture group and 51.6% in the Western medication group after four weeks of treatment and 90.6% in the acupuncture group and 77.4% in the Western medication group after eight weeks of treatment. There were statistically significant differences between the two groups (P<0.05). Patients in the acupuncture group occasionally had acupuncture syncope and local blood stasis. Both groups had no special discomforts.ConclusionJin’s eye three-needle acupuncture has a quick and definite therapeutic effect on acquired paralytic strabismus.

Objective 99Tcm-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime(HL91),a new type of hypoxic agents,accumulates in tumor hypoxic tissue specifically.The aim of this study was to evaluate the value of 99Tcm-HL91 imaging in the diagnosis of bone metastasis.Methods Nineteen cases with bone metastasis(without any treatment)and 8 cases with benign lesions underwent SPECT imaging at 4 h after injection of 740 MBq of 99Tcm-HL91 along with 99Tcm-methylene diphosphonic acid(MDP)imaging.Regions of interest(ROIs)were drawn in tumor tissue and contralateral normal tissue respectively,and the radioactivity ratios of tumor-to-normal(T/N)were calculated.The t-test was used for data analysis with SPSS 11.0.Results There were visible uptake of 99Tcm-HL91 in 79 out of 85 focuses in 19 patients of bone metastasis;however,there was no obvious uptake of 99Tcm-HL91 in 12 focuses of 8 patients of benign lesions.Significant difference existed between the T/N values of malignant(1.877±0.288)and benign lesions[(0.735±0.236);t=13.065,P＜0.05].However,the T/N value of bone metastasis from lung cancer did not differ with that from prostate cancer(1.915±0.344 and 1.825±0.175,respectively;t=1.378,P＞0.05).Conclusion The results indicated that 99Tcm-HL91 was useful in diagnosing the malignant and benign bone lesions.

Purpose: Observe the varities of lymphocyte subgroup of patients suffering from primary hepatic carcinoma ( PHC) during the treatment by hepatic arterial chemoembolization (TACE) and Hyperthemia Peritoneal Perfusion (THPP). Methods: Twenty seven patients were treated with TACE and THPP a week after TACE. We tested for lymphocyte subgroup three times with the flow cytometry (FCM) for pre-TACE, post-TACE and THPP a month later. The control group, twenty seven patients, were also tested for lymphocyte subgroup three times with the TACE. Results: The lymphocyte subgroup among the patients suffering from PHC were disordered. CD4+,CD4+/CD8+.CD19+NK+ were decreased, (P

0.05 ).On the 5th day after radiation,the body weight in +Gln or -Gln groups was significantly lower than that in control group( P

Objective To observe the effect of glutamine on gut barrier function and antioxidative capacity in rats with radiation damage. Methods A total of 30 healthy Wistar male rats were randomly divided into three groups: control group, GLN enriched group(+GLN) and GLN free group( GLN). Rats in control and GLN group were fed with a basic diet, while 3% GLN was added into the diet of the +GLN group. On the 15th day, rats in GLN and +GLN groups received 9.0 Gy 60 Co ? irradiation in total body. The rate of the gut bacterial translocation and the level of endotoxin, GSH and SOD in the serum were determined at the end of the experiment. Results The rate of bacterial translocation and serum endoxin level were significantly lower in +GLN than in GLN, and GSH and SOD levels in serum were significantly higher in +GLN than in GLN. Conclusion Glutamine supplement is helpful to protect the gut barrier function and improve the antioxidation capacity in rats with radiation injury.

Child ; Child Welfare ; China ; Health Services, Indigenous ; Humans

Objective To explore the effects of intramuscular injection human umbilical cord mesenchymal stem cells(hUCMSC)intramuscular injection on the cardiac function and myocardial ultrastructure in rats with Adria-mycin -induced dilated cardiomyopathy (DCM)rats.Methods One hundred and sixty rats were randomly divided in-to a normal group (20 cases)and DCMgroups (1 40 cases),rats in DCMgroups receiving Adriamycin (2 mg/kg)in-traperitoneally once a week for 8 weeks to establish DCM models.The DCM rats were randomly divided into a model control group (served as model group),the supernatant of hUCMSC group (served as supernatant group),the low -dose hUCMSC group(served as low -dose group),the medial -dose hUCMSC group(served as medial -dose group), and the high -dose hUCMSC group(served as high -dose group).Echocardiography was performed to evaluate the car-diac function,plasma brain natriuretic peptide (BNP)level and serum cardiac troponin I (cTnI)level were detected by enzyme -linked immunosorbent assay kit;light microscope and transmission electron microscopy (TEM)were used to observe the ultrastructure of myocardium.Results Rats in the DCM group showed low spirit,declining food intake, progressive emaciation,slow growth,hair loss and ascites.After the intramuscular injection of hUCMSC,the above symp-toms of rats in the low -dose and the medial -dose hUCMSC groups were improved significantly.Before the administra-tion of hUCMSC,the left ventricular ejection fraction (LVEF)[(64.53 ±2.61 )%]and the left ventricular fractional shortening (LVFS)[(30.80 ±2.1 1 )%]were significantly decreased in the DCMgroup compared to those of the con-trol group[(79.67 ±3.02 )%,(43.08 ±3.1 5 )%,all P <0.01 ].After the administration of hUCMSC,LVEF [(75.5 ±7.4)%,(74.0 ±6.1 )%]and LVFS[(40.8 ±3.8)%,(40.2 ±5.0)%]were significantly increased in the low -dose and the medial -dose group compared with those of the model group [(65.8 ±4.5)%,(30.2 ± 2.9)%,all P <0.01 ].The concentration of plasma BNP level [(438.3 ±82.2)ng/L,(341 .7 ±68.9)ng/L]and serum cTnI level [(375.9 ±1 1 0.9)ng/L,(355.9 ±55.6)ng/L]were significantly decreased compared with those of the model group [(449.9 ±91 .8)ng/L,(425.9 ±42.6)ng/L,all P <0.05].The findings of HE staining showed that cardiomyocytes were orderly arranged,edema decreased and cell nucleus homogeneously stained in the low -dose and the medial -dose group.The outcomes of TEM revealed that the ultrastructure of cardiomyocytes was improved in the low -dose and the medial -dose group compared with that of model group,and the cardiomyocyte sarcolemma re-mained intact,and the swelling of mitochondria ameliorated and the cristae of mitochondria remained clear.Conclusions Intramuscular injection of hUCMSC could significantly increase LVEF and LVFS in the Adriamycin -induced DCM rats,and decrease the plasma BNP levels and the serum cTnI levels,attenuate the myocardial pathological damage and improve myocardial ultrastructure.