Objective To analyze the factors of Protein A/G affinity column,influencing purification effect,and get the best condition to improve application effect of Protein A/G affinity column. Methods We Purified anti-HCV-IgG serum with different disposal modality,different sample input modality and different application number of affinity column before detecting and analyzing the purified samples. Results The Protein A/G affinity column had the best purified effect after using saturated ammonium sulfate to first purification,which increased the affinity column adsorption effect within 30 minutes adsorption. Conclusion Using antibody with first purification and adding the adsorption time could improve utilization rate of affinity column and prolong the service life.

Air ; analysis ; Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Hydrocarbons, Chlorinated ; analysis

OBJECTIVE:To explore the relevant factors that led to the adverse drug reaction(ADR)of netilmicin.METHODS:ADR cases resulted from netilmicin reported in12kinds of Chinese periodicals from1998to2003were analyzed statistically.RESULTS:The ADR of netilmicin involved multiple organs and systems,the clinical manifestation were compli-cated and diversified,which manifested mainly on the renal toxicity,toxicity of nervous system and allergic reaction.CON-CLUSION:Clinician and pharmacists should be alert to the ADR of netilmicin and keep using drugs rationally.

Objective To establish a method for determination of total hardness in drinking water by using automatic potentiometric titrator.Methods Dynamic equivalence point titration(DET)and monotonic equivalence point titration(MET)mode was used to determine the high total hardness and low total hardness of drinking water samples respectively.Results Used DET mode to determine the high total hardness,the relative standard deviation(RSD)was 0.69%～1.72% and the recovery rate was 101.5%～102.2%.Used MET mode to determine the low total hardness,RSD was 3.49%～4.00% and the recovery rate was 95.8%- 103.6%.Conclusion This method is rapid,simple,accurate,precise and applicable to the determination of total hardness in drinking water in low and high levels.

ObjectiveTo investigate the effect of early electro-acupuncture treatment on prevention of post-operative adhesions.Methods40 rabbits were randomly divided into 5 groups: normal control group, model group 1, model group 2, early electro-acupuncture group and later electro-acupuncture group with 8 animals in each group. Muscle adhesion model was established by operation. The early electro-acupuncture group was received the treatment from the 11th to 20th day after the operation; later electro-acupuncture group was from the 21st to 30th day after the operation. The weight, gait and range of motion (ROM) of animals were observed and analyzed.ResultsThe standing, walking and ROM of animals of the early electro-acupuncture group improved; but only few gait indexes improved and ROM not changed in the later electro-acupuncture group.ConclusionThe early electro-acupuncture treatment can prevent the post-operative adhesion of muscles, but not having effect on formed adhesion.

The new generation cyclooxygenase (COX-2) inhibitor could reduce the gastrointestinal side effect of NSAID drugs, but eventually increase the cardiovascular risk, because its selective inhibition of COX-2 induces the imbalance between PGI2 and TXA2 and the reduction of vasodilatory NO. Under pathological conditions, active oxygen species (O2-*2, etc) were used to induce endo- thelial dysfunction, activate NF-κB to induce expressions of pro-inflammatory cytokines IL-1β and TNF-α, increase ET-1, TXA2 with vasoconstrictor effect, reduce PGI2 and NO with vasodilatory effect, generate further oxidative damage together with NO, and reduce the bioavailability of NO. NO-NSAIDs and NO-Coxibs drugs raised the level of NO by introducing NO-donor (ONO2). NSAIDs drugs enhanced the anti-inflammatory activity of COX-2 and reduced gastrointestinal side effects by inhibiting selectively COX-2. If antioxidant structures with active ingredients of traditional Chinese medicines were introduced to improve the antioxidant activity of NSAIDs, they could scavenge the active oxygen species to protect the normal function of vascular endothelia and enhance the bioavailability of NO, which is conducive to enhance the cardiovascular safety of cyclooxygenase (COX-2) inhibitor.
Anti-Inflammatory Agents ; therapeutic use ; Biomarkers, Pharmacological ; Cardiovascular Diseases ; drug therapy ; enzymology ; immunology ; Cyclooxygenase 2 ; immunology ; Cyclooxygenase 2 Inhibitors ; adverse effects ; therapeutic use ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; NF-kappa B ; immunology ; Reactive Oxygen Species ; immunology ; Tumor Necrosis Factor-alpha ; immunology

Air Pollutants, Occupational ; analysis ; Carbaryl ; analysis ; Chromatography, High Pressure Liquid ; methods ; Workplace

Objective:Discuss the diagnosis values on the upper digestive tract staininged by the endoscopy.Methods:After spraying straining on the 72 cases below parts using stomach endoscopy,who are suffering from esophagus,stomach disease,and duodenal,observe the staining struction and take some samples.Results:Take samples from Lugol's iodine unstaininged but areas on the 18 esophagus disease,the pathobology shows that 9 suffering f from esophagus carcinomas,3 Barrett's esophagus,6 chroinc esophgitis.Taking the staining areas as samples from 29 diseases (it is 80.55% among 36 disease suffering from methylene blue staining stomach),it appears that 5 are suffering from stomach cancer,21 are intestinal metaplosia and gastric dysplasia.Among 18 staining on bulbs of duodenal ulcer,11 unstaininged are checked gastric metaplasia (61.11%).Conclusions:The endoscopy staining possesses the ability of enlarging the endoscopy diagnosis.The method is simple and safe,it is worthy of clinical application and extending.

AIM: To investigate lipoprotein lipase gene HindⅢ polymorphism and its relationship with serum lipids and apolipoprotein, serum HDL subclasses in patients with hyperlipoidemia. METHODS: Lipoprotein lipase gene HindⅢ polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 152 hyperlipoidemia patients and 128 healthy subjects were determined by two-dimensional gel electrophoresis conjunction with immunodetection method. RESULTS: H+H+ genotype and allele H+ in hyperlipoidemia and control groups were both the highest. In hyperlipidemia group, H+H+ genotypes tended to be higher than that in control group, while H+H- and H-H- genotypes were significantly lower (P

Objective To analyze the characteristics of gastric stromal tumors(GST)under endoseopic ultrasonography(EUS)according to its aggressive risks.Methods The clinical data of 36 patients with GST,who underwent surgery from July 1997 to July 2007,were analyzed retrospectively.All the patients underwent EUS before operation and were classified according to Fleether's 4-tier system to predict the aggressiveness of the tumors.The features of the tumor under EUS including its size,ulceration,border,echo charateristies and growth pattern were recorded and the difference between each tumor group were analyzed by ANOVO and rank sam test.Results The mean maximal diameter of GST Was 7.3 cm(range 1.0-20.0 cm),and the size of tumors with hiisher aggressiveness risk Was significantly larger than that with lower risk(P＜0.01).The mucosal ulceration,obscure border,irregular shape and echo heterogeneity were more commonly seen in the groups with higher risk(P＜0.05).There Was no difference in the internal echo and growth pattern of GST between different groups.Conclusion EUS features are useful in differentiation of the aggressiveness risk of the GST.and guide the management of the tumors.