Complications of artificial joint replacement include infection,aseptic loosening,prosthesis dislocation and breakage,and deep venous thrombosis. Type 2 diabetes mellitus induces capillary pathological changes,tissue blood supply decrease,leading to general and local resistance reduction. Type 2 diabetes mellitus is always complicated by heart,renal,or cerebrovascular complications,which contributes to high infection rate and frequent complications after artificial joint replacement. In addition,incidence of postoperative pulmonary infection and urinary tract infection is increased,and wound healing is delayed. Deep venous thrombosis is a common complication of diabetic patients following artificial joint replacement,which increases the complexity of perioperative treatment. Blood glucose instability may induce circulation function instability and increase indefinite factors during anesthesia. Therefore,blood glucose control is important to reduce risks in artificial joint replacement for diabetic patients.

The character, classification, scoring, diagnosis and treatment of abdominal injuries after the Wenchuan earthquake were analyzed based on the data collected in Chengdu and Dujiangyan. Most of the abdominal injuries were closed injury, and complicated with injuries in other organs. All the injured persons were classified into different groups according to the degree of injuries, so as to determine the optimal sequence of treatment. Open abdominal injuries were comparatively easy to diagnose, while the diagnosis of closed abdominal injuries was difficult and consisted of preoperative diagnosis and intraoperatire exploration. The principle of damage control surgery should be well followed in rescuing the persons with severe abdominal injuries.

OBJECTIVE:To study the interaction between bovine serum albumin(BSA)with lamivudine,efavirenz,tenofovir and its mechanism. METHODS:Fluorescence spectroscopy was used to determine the interaction between BSA with different con-centrations of lamivudine,efavirenz,tenofovir under different temperatures. The fluorescence intensity of them were determined re-spectively;quenching constant(KSV),apparent quenching constant(Kq),binding constant(KA),binding site(n),thermodynamic enthalpy change(ΔH),free energy diversification(ΔG)and entropy change(ΔS)were calculated according to Stern-Volmer equa-tion and so on. Molecular docking model of 3 drugs with BSA was established by using Sybyl 6.7 Flex X model. RESULTS:Kq for the interaction between 3 drugs with BSA were all higher than 2.0×1010 L/(mol·s),and were decreased with the increase of temper-ature;all n were close to 1,and thermodynamic functions ΔG<0,ΔS<0,ΔH<0. Molecular docking model showed that 3 drugs were mainly bound with BSA at Sudlow Ⅰ subdomain site. CONCLUSIONS:There are the interaction between 3 drugs with BSA;fluorescence quenching mainly manifests as static quenching;binding reaction belongs to spontaneous molecular action pro-cess;binding force mainly includes hydrogen bond and Van der Waals'force. The result of fluorescence experiment is consistent with those of molecular docking,and they complement each other.

This study was aimed to explore effect of Gui-Zhi Fu-Ling W an (GZFLW) on HIF-1α and vascular en-dothelial growth factor (VEGF) in patients with endometriosis. A total of 68 endometriosis patients in the First Peo-ple's Hospital of Xining from June 2011 to June 2013 were included. All patients received treatment of GZFLW, once a day. After one-month treatment, the treatment efficacy was analyzed. The endometrial tissues (both ectopic endometrium and eutopic endometrium) were obtained before and after treatment. The protein levels of HIF-1α and VEGF as well as the relationship of HIF-1α and VEGF were analyzed by SP immunohistochemistry. The results showed that the total treatment efficacy was 86.76%. Compared with pretreatment, the posttreatment protein levels of HIF-1α and VEGF were significantly reduced (P< 0.05). The decreasing of VEGF protein level and HIF-1α expres-sion level were positively correlated (P< 0.05). It was concluded that GZFLW had better efficacy in the treatment of endometriosis. One of the reasons may be the downregulation of protein levels of HIF-1α and VEGF in endometriosis tissues.

Objective To establish a reversed HPLC method to determine the contents of gatifloxacin and tinidazole simultaneously. Methods ODS C_ 18 column (4.6mm?250mm,5?m) was used as an analytical column. The mobile phase consisted of 15% acetonitrile, 25% of methanol and 60% of 0.05mol/L KH_2PO_4 buffer,which contained 0.5% triethylamine and was adjusted to pH 3.5 by phosphoric acid. The flow rate was 1ml/min. The detection wavelength was 318nm. The column temperature was 30℃. 20?l of diluted sample solution was injected to the sampler. Results The calibration curve of gatifloxacin was linear in the range of 2~20?g /ml (r=0.999 6) ,and that of tinidazole was 2~20 ?g /ml (r=0.999 9). The recovery rates of them were 100.1% and 100.4%,respectively. RSDs of both drugs were less than 1.1 %. Conclusions The method is simple,accurate and sensitive and can be used to determine the two components simultaneously.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

OBJECTIVE According to the development of nosocomial infection management in China,we design a series of nosocomial infection surveillance and management system,in order to work effectively in real time to control nosocomial infection outbreak,standardly collecting and analyzing the surveillance data of the critical department/area and risk factors for nosocomial infection,so a scientific management for nosocomial infection can be realized.METHODS Adopting C/S framework,the backstage supporter adopted the large-scale database of SQL SERVER 2000 edition,the front application program used PowerBuilder7 programming.The application software where collected all data about nosocomial infection information was integrated into the each work station of the information system,in order to form nosocomial infection surveillance and management network related to any department of the hospital.RESULTS System could come down with the hospital infection case which every hospital department could find to check,put in order,report,count,analyze,have a look around,inquire about,feedback,form the monitoring chart with excellent pictures and texts,and make the better prognosis of the nosocomial infection outbreak.CONCLUSIONS Beijing nosocomial infection surveillance and management system is a medical quality control system of a hospital.This software could afford scientific and standard data for the health care institution and administration to realize the primary information of nosocomial infection and make decisions.

Objective To investigate the effects of CO 2 pneumoperitoneum on circulation, P ET CO 2 and blood gas during laparoscopic hepatectomy. Methods Fourteen patients underwent laparoscopic hepatectomy with CO 2 pneumoperitoneum. The MAP, HR, CVP, P ET CO 2 , airway pressure and blood gas were monitored respectively before, 30min, 60min, 120min after pneumoperitoneum, and 10min and 30min after deflation of the abdomen. Results The HR, P ET CO 2 , PaCO 2 , and plood K + were significantly higher 30min after pneumoperitoneum compared with the values before pneumoperitoneum (P

Objective:To study the effects of n-Butanol Extract (BEX)of Xanthoceras Sorbifolia Bunge on rat model with adjuvant arthritis(AA) in rats and its mechanism .Methods: Rat model of adjuvant arthritis(AA) was induced by FCA. Hind paw swelling was observed to evaluate the effect of BEX and phagocytic function of monocyte-macrophage system, antibody level and delayed hypersensitivity were examined to explore its mechanism. Results: BEX can inhibit the primary and secondary hind paw edema and improve the general symptoms (P

Tumor recurrence is the main factor affecting the postoperative prognosis of hepatocellular carcinoma.The forecast of tumor recurrence by monitoring biomarker can effectively improve the prognosis of such disease.To improve the detection rate of early cancer recurrence can be achieved by detecting the level of the alpha-fetoprotein (AFP),Lens culinaris agglutinin-reactive fraction of AFP (AFP-L3),des-γ-carboxyprothrombin (DCP),or combined detection.In recent years,the researches on the miRNA and Long Noncoding RNAs (LncRNA),which are key regulators in the upstream of signal pathways,could provide a new way for HCC recurrence monitoring.The combined detection of biomarkers in different control levels,maybe is the effective measure to improve the tumor recurrence detection rate,and is also the focus of the further research.