OBJECTIVE: To prepare and establish a release rate method for the determination of ganciclovir lung-targeted microcapsules.METHODS: The microcapsules were prepared by compound emulsion-dispersion solvent evaporation method.The in vitro drug release amount and release rate of the microcapsules were detected by UV spectrophotometry.RESULTS: The microcapsules were regular in shape with an average particle size of 13.87 ?m.The linear range of ganciclovir was 2～20 ?g?mL-1(r=0.999 9) and its average recovery was 99.96%(RSD=1.68%).The entrapment efficiency of the microcapsules was 63.85% and the drug release amount in vitro reached 40% in 24 hours.CONCLUSIONS: The microcapsules show significant sustained release character.

The technology of processing carbonized Cephalanoplos segetum was optimized by using orthogonal experiment design. Results showed that the best way is to stir-fry in a pan for 5 min at 210℃. The contents of trace. elements in the prepared specimen were found to be significantly increased.

Objective To investigate the effects of panax notoginseng saponins(PNS) on the expression of IL-10 and TNF-α in the brain after focal cerebral ischemia-reperfusion injury in rats,and the mechanism was explored.Methods 30 male adult Sprague-Dawley rats were randomly divided into sham-operation group,ischemia-reperfusion group and PNS treatment group,10 rats in each group.The middle cerebral artery occlusion(MCAO) was established with ischemia-reperfusion group.The rats received continuous gastric lavage for 4 weeks,who were observed for 3 days and then killed to the detect the expression of IL-10 and TNF-αin ischemic region by ELISA,immunohistochemistry and RT-PCR.The behavioral ability score in rats were tested.Results Compared with the sham-operation group and ischemia-reperfusion group,the expression of IL-10 was increased,while the expression of TNF-α was decreased significantly in PNS treatment group,behavior score of PNS treatment group increased significantly than that of ischemia-reperfusion group.Conclusion The panax notoginseng saponins can reduce the neurons injury after focal cerebral ischemia-reperfusion injury in rats partly by regulating the expression of IL-10 and TNF-α in the brain.

ObjectiveTo investigate whether miR-25 targets KLF4 to regulate the growth of gastric cancer cells and lucidate the mechanism of miR-25 producing functional effects in gastric cancer cells. Methods The expression levels of miR-25 and KLF4 were detected by qRT-PCR in 35 pairs of gastric cancer tissues of patients. Target genes of miR-25 were predicted using Targetscan and verified though dual luciferase activity assay and west-ern blot. MTT assay and clone formation assay were detected after downregulated miR-25 and overexpressed KLF4 respectively. Results miR-25 was highly expressed in gastric cancer tissues with KLF4 downexpresstion. Downreg-ulated miR-25 and overexpressed KLF4 respectively can significantly reduce the proliferation of gastric cancer cells,the results were opposite when overexpression of KLF4. Conclusion miR-25 promotes gastric cancer cells proliferation by targeting KLF4.

Anemia, Iron-Deficiency ; blood ; diagnosis ; Biomarkers ; blood ; Child ; Child, Preschool ; China ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Infant ; Male ; Receptors, Transferrin ; blood

Objective To explore the effect of Feier Recuperates Fluid on lipid metablism, fat weight, fatty cell shape. Method Trophism fat rat model was induced by feeding hyperalimentation feedstuff for six weeks in infancy rat. Drugs were administered orally after two week for four weeks. The cholesterol total (TC), triglyceride total (TG) and high-density lipoprotein-cholesterol (HDL-C) contents in the serum were determined. Fat weight was weighted. Number and diameter of fat cells were observed. Result Feier Recuperates Fluid (63.0 g/kg) could reduce fat weight, TC and TG contents, and increase HDL-C. Feier Recuperates Fluid could decrease diameter of fat cells, and increase fat cells number slightly. Conclusion Feier Recuperates Fluid could improve markedly lipid metablism of trophism fat rat.

Objective To investigate the expression level of cyclooxygenase-2 (COX-2) and its effects on cell proliferation and apoptosis in bladder cancer. Methods The immunohistochemical method was used to determine the expression of COX-2 and Ki67 in 54 cases of bladder cancer, 29 cases of adjacent tumor tissue and 10 cases of normal bladder specimen respectively, and the apoptotic levels of the obove three tissues were assessed by counting the number of the cells labeled by TUNEL method. Results The positive rate of COX-2, the mean labelling index of Ki67 and the cellular apoptosis index in the cancer tissues were significantly higher than those in adjacent tumor tissues and normal bladder specimens(P

Blood-Brain Barrier ; radiation effects ; Capillary Permeability ; radiation effects ; Electromagnetic Radiation ; Humans

Cardiovascular Diseases ; psychology ; Humans ; Mental Health

Actins ; metabolism ; Adult ; Carcinosarcoma ; diagnostic imaging ; metabolism ; pathology ; surgery ; Hepatectomy ; methods ; Humans ; Liver Neoplasms ; diagnostic imaging ; metabolism ; pathology ; surgery ; Male ; Tomography, X-Ray Computed ; Vimentin ; metabolism