Objective To study the efficacy of sodium profile combined with ultrafiltration profile in preventing intradialytic hypotension and changes of the sodium level in blood plasma after hemodialysis. Methods 10 patients with maintenance hemodialysis were chosen randomly. Every patient underwent with conventional hemodialysis treatment and sodium profile combined with ultrafiltration profile treatment 5 times alternatively. And then the blood pressure changes. HR changes, ultrafiltration volume changes and clinical symptoms were observed during treatments. And also after HD, the sodium level in blood plasma was observed. Results The blood pressure decrease of sodium profile combined with ultrafiltration profile evidently reduced the conventional HD treatments( P＜0.01 ).Ultrafiltration volume of the former evidently increased compared with that of the latter(P＜0.01 ). The sodium level in blood plasma after HD was not significantly changed compared with before HD(P＞0.05). Conclnsion Sodium profile combined with ultrafiltration profile diminishes the episodes of hypotension evidently and increases the ability to endure sufferirgs to patients and does not increase the sodium level in blood plasma. So it is an effective medical treatment.

OBJECTIVE:To observe the efficacy and safety of alprostadil combined with tanshinone ⅡA in the treatment of pa-tients with early chronic kidney disease(ECKD). METHODS:138 ECKD patients were randomly divided into group A,group B and group C,46 cases in each group. On the basis of conventional treatment,group A received 40 mg TanshinoneⅡA sodium sulfonate in-jection,adding into 250 ml 0.9% Sodium chloride solution,ivgtt,qd+10 μg Alprostadil injection,adding into 100 ml 0.9% Sodium chloride solution,ivgtt,qd;group B received the same dose with Alprostadil injection;group C received the same dose with Tanshi-noneⅡA sodium sulfonate injection. All patients were treated for 14 d. Clinical efficacy,laboratory indexes [24 h urine protein,serum creatinine(SCr),total cholesterol(TC),triglyceride(TG),blood urea nitrogen(BUN),uric acid(UA)] in 3 groups before and af-ter treatment were observed,and the incidence of adverse reactions was compared. RESULTS:The total effective rate in group A was 97.83%,which was significantly higher than group B(80.43%)and group C(76.09%),the differences were statistically significant (P<0.05). Before treatment,there were no significant differences in 24 h urine protein,SCr,TC,TG,BUN and UA among 3 groups (P>0.05);after treatment,all above-mentioned indexes significantly reduced,and group A was lower than group B and group C,the differences were statistically significant(P<0.05). There was no significant difference in the incidence of adverse reac-tions among group A(10.87%),group B(6.52%)and group C(8.70%)(P>0.05). CONCLUSIONS:Alprostadil combined with tanshinoneⅡA shows good efficacy in the treatment of ECKD,it can improve renal function in patients with good safety.

Objective To observe the changes in the pathological diagnosis rate of Graves'disease(GD) and Hashimoto's thyroiditis(HT)in the past 28 years and to probe its relationship to the environmental factors. Methods Statistic analysis was performed on data of thyroid disease,GD,HT and the complications of HT in Department of Pathology(1978-2005)in General Hospital of Tianjin Medical University with Run test,Cox-stuart test,ANOVA,t or t'test and Chi-square test.Results In 28 years there were 243 403 biopsies(average 8 693/ year)including 6 771 cases of thyroid disease(27.8‰),216 cases of GD(0.89‰),352 cases of HT (1.45‰).The biopsy rate of thyroid disease and GD showed a descending tendency since 1982 but an ascending tendency from 1995,furthermore,with GD it showed a descending tendency from 2001 to 2005 once more.While the biopsy rate of HT and GD+HT showed a descending tendency from 1986 and an ascending tendency after 1996,being 1-4 years later than thyroid disease and GD.The biopsy rate of GD+HT and the proportion of HT showed a descending tendency in 2002-2005 and 2003-2005 respecitvely.The average age of total and female patients with GD showed a descending tendency from 1997.The average age of GD was 13.7 years younger than HT,the patients of GDⅠ-type andⅡ-type being younger thanⅢ-type.The average age of lymphoid type(L), oxyphilie epithelium type(O),pronounced epithelium destruction type(P)in HT increased sequentially.In 28 years,there were more female patients with GD and HT,especially with HT.There existed 7.95% hyperthyroidism,6.25% nodular goiter,2.27% carcinoma in HT(more in L-type)and 2.56% hypothyroidism in HT(more in P-type).Conclusion Within 28 years,according to the pathological diagnosis rate,GD,HT,and GD+HT showed phasic tendencies related to drinking low iodine water and the implement of universal salt iodization policies in 1994 and 2000.Therefore,on the levels of genetics and environment,the factors that influence autoimmunity of thyroid gland could not exclude the variations of iodine intake,infection,affection or emotional stress.The mechanism of the changes in the pathological diagnosis rate needs further study.

Adrenal Cortex Hormones ; administration & dosage ; therapeutic use ; Arthritis, Juvenile ; drug therapy ; Humans ; Injections, Intra-Articular

Acute Disease ; Case-Control Studies ; Child ; Child, Preschool ; Female ; GATA3 Transcription Factor ; genetics ; Humans ; Infant ; Interferon-gamma ; genetics ; Interleukin-4 ; genetics ; Male ; Mucocutaneous Lymph Node Syndrome ; blood ; genetics ; immunology ; RNA, Messenger ; Reverse Transcriptase Polymerase Chain Reaction ; T-Box Domain Proteins ; genetics ; Th1 Cells ; immunology ; Th2 Cells ; immunology

AIM:To study the direct reprogramming method of mouse embryonic fibroblasts (MEFs) conver-ted into induced neural stem cells (iNSCs).METHODS:Sox2-infected MEFs were cultured in NSCs culture medium for 10 d.Subsequently , repeated suspension and adherent culture were performed for 3 times for the purification of iNSCs .The iNSCs were cultured in suspension medium .Real-time PCR was used to detect the expression of neural stem cell marker genes and pluripotent marker gene .In vivo, iNSCs were microinjected into the mouse cerebral cortex .Immunofluorescence was performed to detect the expression of neural stem cell , neuron, oligodendrocyte and astrocyte markers in vitro and vivo. RESULTS:A variety of neural stem cell marker gene expression was significantly increased in iNSCs detected by real -time PCR.Immunofluorescence confirmed that iNSCs expressed nestin and differentiated into neurons , oligodendrocytes and as-trocytes in vitro and vivo.CONCLUSION:Sox2 is sufficient to trigger the direct reprogramming from MEFs to iNSCs .iN-SCs have the ability of self-renew and 3 differentiation potentials in vivo and vitro.iNSCs are the suitable seed cells of SCI .

OBJECTIVE:To put forward the tentative idea of the application of the access and benefit-sharing regime in the field of Chinese medicine.METHODS:The existing problems of protecting biological diversity in the field of Chinese medicine and what problems the access and benefit-sharing regime could solve were analyzed.RESULTS & CONCLUSION:Our preliminary idea for applying access and benefit-sharing regime in the field of Chinese medicine is to set up a special department for negotiation,identify the rights and subject,construct registration system and informed consent procedure,improve the source disclosure system and add the benefit-sharing regulation.

Objective To explore the role of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in hyperoxia-induced lung injury in preterm rats. Methods At the 2 nd postnatal day Sprague-Dawley preterm rats were randomly assigned to air group and hyperoxia group (exposed to about 85% of O 2). At 3,7,14 and 21 days after exposure, six rats of each group were used to assess lung histologic changes and expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in lungs by immunohistochemistry. At 3,7 and 14 days after exposure, gelatinase activity in bronchoalveolar lavage fluid (BALF) of another six rats in each group by gelatin zymography was examed. Results Except 3 d after exposure, hyperoxia group showed lung injury characterized by subacute alveolitis and inhibition of lung development. Expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in hyperoxia group was stronger than that in air group at every time (P

Objective To understand the sanitary quality of cosmetics in Wuhan. Methods The sanitary quality of cosmetics including hair care cosmetics, skin care cosmetics, beauty cosmetics, perfumes and cosmetics for special use sampled from 23 cosmetics manufactures and 15 large-scale whole-salers and retail traders in Wuhan were examined during 1996～2001. Results The annual qualified rates of cosmetics were 93.49%～98.69% in 1996～2000. It was 95.65% for hair care cosmetics, 92.41% for skin care cosmetics, 98.13% for beauty cosmetics, 100% for purfumes and 95.56% for special use cosmetics respectively. They also were 93.62%～98.69% for microbiological indexes and 99.17%～100% for chemical indexes respectively. The total count of bacteria of skin care cosmetics seriously exceeded the related sanitary standard. Conclusion The main problem of the sanitary quality of cosmetics was the microbiological pollution.

Objective To Observe the influence of Zaoshi Jiedu Zhupi Sanjing (ZPSJ) Recipe on insulin sensitivity, serum resistin and inflammatory factors related to the effects of Chinese herbal compound in type 2 diabetic patients with insulin resistance. Methods A single-blind randomized controlled clinical trial was conducted. 80 patients with 2 type diabetic patients with insulin resistance were recruited into a control group and a treatment group. The treatment group was treated with ZPSJ Recipe, and the control group was treated with metformin. Results In each group before and after treatment: blood glucose, serum insulin levels, HOMA-IR were significantly decreased (t=7.14, P<0.01 ) ; between the two groups, FPG, FINS showed no significant difference, while TG TCH, HOMA-LR showed significantly differences (t=2.62, P <0.05 ). Within the two groups before and after the treatment: serum IL-6, TNF-a, hs-CRP were decreased in treatment group, (t= 1.85, P<0.05 ) , while TNF-a, hs-CRP were decreased in the control group (t=2.56, P<0.05 ) , while IL-6 did not change significantly. The serum resistin levels were not statistically changed in both groups before and after treatment. Conclusion ZPSJ Recipe can regulate blood sugar, blood fat and improve insulin sensitivity in type 2 diabetic patients with insulin resistance.