Adult ; Histiocytosis, Langerhans-Cell ; Humans ; Male

Poly(U)-binding-splicing factor 60 KDa (PUF60), a U2-related splicing factor that facilitates 3′splice-site recognition at the early stage of spliceosome assembly.High expression of PUF60 is related to the occurrence of multiple tumors, such as colorectal cancer, ovarian cancer, gastric cancer, liver cancer, et al.PUF60 can regulate the expression of c-myc through the core-human transcription factor Ⅱ basal transcription factor.Anti-PUF60 antibodies are detected in the sera of patients with early-stage and recurrent tumor, and the levels are significantly decreased after the operation.PUF60 can be used as a combined or independent test index for the diagnosis of cancer, which can be a potential new target for gene therapy.

Objective:To provide some suggestions for improving the pharmaceutical advertisement supervision system and standardizing the pharmaceutical advertisement market. Methods: Literature reviews, statistical analysis and interviews were adopted to study the status quo and difficulties of pharmaceutical advertisement supervision and pharmaceutical advertisement trends. Results:The top-level design of pharmaceutical advertisements supervision sys-tem has two defects:the separation between approval and penalty and the obstacles to cooperation at all levels of the drug administration. The worsening status quo of illegal drug advertising has elicited concern from scholars and regu-lators. However, there are still some difficulties in the supervision of drug advertisers, advertising agents, and pub-lishers. Conclusion:It is suggested that the drug advertising regulation system should be perfected by strengthening information supervision, establishing a coordination mechanism and consolidating self-discipline consciousness. Addi-tionally, laws and regulations should be promptly revised as an essential way to improve the effects of supervision.

OBJECTIVE:To establish the HPLC method for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills. METHODS:It was performed on Hypersil-ODS C18 with the mobile phase of acetonitrile-water(14∶86,V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,the column temperature was room temperature and the volume was 10 μl. RESULTS:The linear range was 16.12-80.6 μg/ml for paeoniflorin(r=0.999 6)and 6.08-30.4 μg/ml for liquoritin(r=0.999 8);the RSDs of precision,repeatability and stability tests were all less than 2.0%;the average recovery was respectively 98.51%(RSD=1.94%,n=6)and 98.08%(RSD=1.29%,n=6). CONCLUSIONS:The method is simple,accurate and reliable, and can be used for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills.

Objective To establish a method of preparing metoprolol succinate sustained-release tablet and its content determination. Methods The formulation was optimized through the orthogonal design test by using release rate of the drug as an indicator.The different batches of metoprolol succinate sustained-release tablets were determined by HPLC. Results The tablets could release drug steadily and slowly as designed,which was similar to imported tablets. The linear range of metoprolol succinate was 10-70 μg?mL-1( r=0.999 8) . Conclusion The releasing rate of metoprolol succinate sustained-release tablet prepared in optimum condition can meet the requirement. This preparation technology is simple, the assay method is rapid, sensitive and reproducible.

OBJECTIVE: To provide references for Chinese drugstores to carry out private brand strategy. METHODS: The problems existing in the practice of private brand in Chinese drugstores were analyzed,and the basic conditions and effective premium approaches for private brand of pharmaceutical chain store were studied through a detailed analysis of consumer’s psychology and action. RESULTS & CONCLUSIONS: The problems existing in the practice of private brand in Chinese drugstores included the non-guaranteed quality assurance ability results from low price marketing and enterprise’s lack of cohesiveness results from enforcement on salesman to recommend products for customers etc. The main conditions to promote premium on private brand were to possess a consummate quality assurance system for products and services. The main approaches toward premium are to construct product line with drug brand image as foundation and to optimize the organization mode of the private brand management.

Objective To confirm the expression of Aquaporin2,3,4(AQP2,3,4,water channel protein) in rats'endolymphatic sacs(ES)and kidneys and to investigate and to compare the effects of anti-diuretic hormone(AVP) and DDAVP([deamino-Cys1,D-Arg8]-Vasopressin,V2-receptor agonist)on the expression of AQP2 in rats' ESs and kidneys.Methods 30 healthy Swards white rats were divided into the negative control,AVP group and dDAVP group respectively.The animals were cardiacally perfused.The temporal bones and kidneys were taken out and sectioned by means of the paraffin-embedded technique.The sections of ESs were labeled with fluorescent antibody by immunohistochemical method,and the kidneys' with avidin-biotin-peroxidase complex method(ABC).The expressions of AQP-2 were confirmed in the ESs of the rats while the different effects of the AVP and DDAVP on the ESs and the kidneys were observed.The slides used were analyzed by the image-analyzer and the subsequent data were statistically studied.Results In the cytomembrane and cytoplasm of ESs' epithelia,the constant and clear fluorescent reaction could be observed in the normal control group with the first antibody of AQP2.Significant feeble fluorescent reaction of the first antibody of AQP-2 was revealed in AVP group and DDAVP group.In comparison with the control group,significant stain was found in AVP group and DDAVP group.Noted were also lower gray(P

0.05).The correlation of reduction of intra-disc pressure and accumulation of ablation time was positive.Compared with intra-disc pressure in control group,the pressure change of all ablation groups was statistically significant(P

OBJECTIVE:To discuss problems existed in clinical pharmaceutical care of our country.METHODS:The root causes of the problems in the clinical pharmaceutical care of Chinese were analyzed.RESULTS&CONCLUSION:The legalized management of pharmaceutical care of China should be speed up;“practitioners pharmacist law”(or“pharmacist law”)should be formulated so as to clarify clinical pharmacist responsibility,obligation and legal position and to guarantee pharma?cists'legal interests.

Chinese medicine has certain advantages in treating the disease of children ADHD which caused by brain dysfunction. According to the physiological characteristics of "kidney is often insufficient" in children, and the close relations among kidney, marrow, essence and brain, we put forward the pathomechanism hypothesis that growth retardation of spinal marrow caused ADHD, and thought that the def iciency of kidney essence, growth retardation of spinal marrow, and imbalance of yin and yang (yang superabundant and yin insufficient), are the keys of pathomechanism of ADHD. Accordingly, the tonifying kidney and replenishing essence treatment principle was established, and YiZhiNingShen granule was made which had been conf irmed effectively by clinical and experimental research.