Objective To analyze the epidemiological characteristics of public health emergencies and the situation of report and control of public health emergencies in Qijiang District of Chongqing M unicipality during 2009-2013 to provide a scientific ba‐sis for the prevention and control of public health emergencies .Methods The descriptive epidemiology method was used to analyze the directly network reported data of the public health emergencies in Qijiang District during 2009-2013 from the National Emer‐gency Public Health Emergencies Reporting Management System .Results Total 47 public health emergencies were reported in Qi‐jiang District during 2009-2013 ,including 994 cases of caused disease and 1 case of death (food poisoning) .Most of the emergen‐cies were caused by infectious diseases events ,accounting for 82 .78% (39/47) ,food poisoning and other public health emergencies (dog bites) accounted for 2 .13% (1/47) and 14 .89% (7/47)respectively .Respiratory disease events accounted for 78 .72% (37/47) of total infectious diseases events ,the majority were influenza A (H1N1) flu and epidemic parotiditis .The high‐incidence seasons were from April to May(accounting for 34 .04% )and October(40 .42% ) .The most cases happened to the school (especially infec‐tious diseases) ,accounting for 80 .85% of the total events .Conclusion The respiratory infectious disease in the prevailing season is the priority of prevention and control on public health emergencies in Qijiang District .Attentions should be highly paid to the pre‐vention and control of public health emergencies (especially in rural schools);meanwhile ,the dog management and propaganda of prevention and control knowledge should be strengthened to effectively eliminate the potential of hydrophobia .

This paper reported that bronchial artery embolization(BAE)had been used to treat 35 non tumorous patients who suffered from recurrent hemoptysis or acute severe hemoptysis after the given medical treatment was proved uneffective in 35 patients,7 patients had recurrent hemoptysis during 1.5～19 months postoperatively and were treated by BAE again,and there was no recurrence of hemoptysis during 7～34 months follow-up.Therefore, the authors considered that repeated BAE was still effective in treating recurrent hemoptysis.

Objective To summarize the clinical experience of serious hyponatremia resulted from applying pitnitrin to massive hemorrhage of gastrointestinal tract patients.Methods we used pitritrion on 2460 such patients and examined some symptoms of the digestive tract and nerve system,monitoring the dynamic state of blood sodium.Results The re- suits reveal that patients in 30 cases turned out to suffer from serious hyponatremia,whese sodium in the serum appeared less than 110 mmol/L,which was 1.22% of the rest subjects'.The relevant symptoms included nausea,vomit,spoor,coma and twitch etc.Following remedies included liquid supply with a high-concentrated sodium,ceasing using pituitrin,in- take of salute foods,and dynamic monitoring on the state of sodium in the serum.All patients recovered in 1 to 3 days without a single failure.Conclusion Thus we reach this conclusion:When applying pituitrin to massive hemorrhage of gastrointestinal tract patients,the above mentioned symptoms should be closely examined,and blood sodium should be timely monitored so that proper treatment should arrive in time.

Objective To explore the effect of osthole on mast cells and expression of STAT5 gene and protein in them. Methods Passive cutaneous anaphylaxis was made in mice to copy eczema model, then the allergic mast cells were separated, and the ovalbumin was used to induce allergy of mast cells. Different concentrations of osthole were used to intervene the sensitized mast cells.Then the sensitized mast cells were divided into blank control group, osthole high-dose group and low-dose group.At the end of the experiment, morphology of the mast cells was detected by immunofluorescence technology.MTT assay was used to detect the influence of drugs on mast cells proliferation. RT-PCR and Western blotting were used to detect the expression of STAT5 gene and protein. Results As compared with blank control group, the number of mast cells in the osthole groups was significantly reduced, cells and nuclei obviously shrank, even apoptosis of some cells could be observed; the inhibition rate of mast cells in osthole groups was significantly increased in concentration-dependent manner ( P<0. 01 ) . As compared with blank control group (2.16±0.57), gene expression of STAT5 was significantly decreased in osthole high-dose group (0.59±0.12) and low-dose group (0.82±0.13) (P<0.01).The protein expression of STAT5 was also significantly decreased in osthole high-dose group and low-dose group as compared with blank control group (P<0.01). Conclusion Osthole can inhibit the proliferation of sensitized mast cells, and reduce the expression of STAT5 gene and protein.

Objective To discuss the value of amnionic fluid lamellar body count(LBC) as a predictor of fetal lung maturity. Methods Amnionic fluid was obtained during the cesarean section of 41 normal term pregnancies. LBC was performed on each specimen, and lecithin-sphingomyelin(L/S) ratio was detected simultaneously. Results (1)Amnionic fluid LBC was (86?43)?10 3 (72.5?10 3~99.4?10 3) in normal term pregnancy. (2) Positive correlation was shown between the amnionic fluid LBC and L/S ratio(r=0.66,P=0.001). Conclusion The LBC is a rapid and accurate method to determine the fetal lung maturity.

Objective: To analyze the risk factors on acute respiratory dysfunction caused death in patients after type A aortic dissection surgery.
Methods: A total of 223 patients who received aorta replacement surgery in our hospital from 2010-01 to 2012-12 were retrospectively studied. 80 patients suffered from post-operative acute respiratory dysfunction including 61 male and 19 female with the mean age of (49.2 ± 11.6) years. Those patients were divided into 2 groups as Death group, n=18 and Survival group, n=62. We analyzed the most relevant risk factors for death, such as gender, age, histories of smoking, diabetes, hypertension, Marfan syndrome;pre-operative acute or chronic dissection, hypoxemia, mal-perfusion, LVEDD and LVEF;CPB time, aortic-clamping time;post-operative ICU retention time, mechanical ventilation time, permanent neurologic dysfunction, pulmonary infection, MACE, renal failure, hypohepatia, septicemia and wound mal-healing, et al.
Results: The early post-operative (< 3 days) respiratory dysfunction rate was 35.8% and the mortality was 22.5%(18/80). The relevant risk factors of death included female gender (P=0.019), haemorrhage (P<0.01), mechanical ventilation time (P=0.011), permanent neurologic dysfunction (P=0.013), pulmonary infection (P=0.001), MACE (P=0.022), renal failure (P<0.01), hypohepatia (P<0.01) and septicemia (P=0.001). Female gender and renal failure were the independent risk factors for respiratory dysfunction caused death in patients after type A aortic dissection surgery.
Conclusion: The occurrence and mortality were high in patients after type A aortic dissection surgery especially in those with female gender and post-operative renal failure.

Objective　The covered tracheal stent in shape of a straight tube or a fork-like tube was placed clinically to treat tracheal stricture and tracheal fistula caused by malignant tumour in order to maintain the normal function respiration and to keep the fistula closed.Methods　The stent was made of stainless steel in shape of "Z"covered with membrane partially or entirely.An instrument with tube in three sheathes was used to place the stent in position.Results　The tracheal stents were placed successfully in 9 cases at first time and the esophageal stents were placed for 6 cases among them in the meantime.Dyspnea of patiants was remarkably alleviated.The tracheal fistula of them was closed effectivelly.Follow-up for 4～12 monthes all cases demonstrated no adverse effects.Conclusion　The application of the covered stent is a safe effective method to treat the tracheal stricture and tracheal fistula.

Objective To analyze the epidemiological characteristics of unintentional injuries among children patients at age of ≤ 14 years so as to provide scientific basis for prevention of the children injury. Methods ICD-10 coding was used to retrospectively analyze the epidemiological characteristics of the children patients with unintentional injuries at age of ≤ 14 years admitted from January to December 2009 and the factors affecting the prognosis were discussed. Results There were 3011 patients, with 1991 males (66.12%) and 1 020 females (33.88%). The patients at ages of < 1, 1-4, 5-9 and 10-14 years accounted for 8.24%, 53.30%, 27.90% and 10.56% respectively. The patients from the rural and urban areas accounted for 56.16% and 43.84% respectively. Trauma was the leading sort of injuries in four age groups, accounting for 70.56%, 62.68%, 89.64% and 87.74% respectively. Fall from height was the first cause of unintentional injuries in four age groups, with proportion for 47.58%,36.39%, 51.90% and 56.29% respectively. Muhinomial logistic regression analysis revealed that hospital stay, therapeutic mode ( operation/non-operation), inhabitancy ( urban/rural), causes of injuries and injury severity on admission were the risk factors affecting the prognosis (P < 0.05 ). The rural areas caused higher risk of disability than the urban areas ( OR = 2. 170 ,P < 0.01 ) and non-operation produced higher risk of death than operation (OR = 6.352,P < 0. 01 ). Conclusions The male patients with unintentional injury are more than the female ones, with the highest incidence rate in children at age of 1-4years. Trauma is the main kind of unintentional injury and the fall from height the leading cause of injury.The difference of the rural and urban areas indicates that correct choice of the preventive measures based oh the condition of the local area can attain effective prevention and control of the unintentional injuries.

Objective To study the protective effects of aqueous extract and alcohol-soluble extract of Isodon lophanthoides var.gerardianus (Benth.)Hara (ILVG) on immunity hepatic injury induced by concanavalin A (Con A) in mice.Methods One hundred and eight NIH mice were allocated into normal control group,model group,ILVG groups of aqueous extract and alcohol-soluble extract (low-,middle-and high-dosage),and bifendate group randomly.In the experimental groups,mice received either ILVG aqueous extract or alcohol-soluble extract (18.20 g?kg-1,9.10 g?kg-1,4.55 g?kg-1 respectively) or Bifendate (45 mg?kg-1) by gastric perfusion daily for consecutive 5 days.In the 5th day,Con A (20 mg?kg-1) was injected into mice via the tail vein 4h after administration.And then the blood was obtained by picking out the eyeball and the serum was separated after 8-hour fasting.The serum levels of ALT and AST were analyzed,the body weight as well as the weight of liver,spleen and thymus were measured,and pathological features of hepatic tissue were observed.Results ILVG can decrease the ALT and AST,restrain the enlargement of liver and the shrinkage of thymus and reduce the necrosis of hepatic tissue.Conclusion Aqueous extract and alcohol-soluble extract of ILVG possess the effects of protecting liver from immunity injury induced by Con A in NIH mice.

Objective To optimize the microemulsion preparation of Fufang Zicao Oil(FZO).Methods The extraction conditions of microemulsion preparation of FZO were optimized by orthogonal experiment;the kinds and contents of surfactants and cosurfactants determined by pseudo-ternary phase diagram were used for the optimization of the prescription of FZO;centrifugation method was used for detecting the stability of FZO.Results The optimum extract conditions were as follows:10-mesh coarse powder of medicinal material,four times amount of solvent(sesame oil),extraction for 25 minutes.With Tween 80 and propylene glycol(7:3)as the surfactant and cosurfactant,and mixing with the oils in the proportion of 20:10,a steady microemulsion of FZO can be obtained after centrifugation by 13000 r? min-1 for 30 min.Conclusion Under the above achieved conditions,the obtained microemulsion of FZO is clear,transparent and steady.