Object To determine the change of benzoic acid contents in decoctions prepared from various combinations of the recipe named SIWUTANG by RP HPLC to provide a reference for the control of the acid level Methods 8 decoctions of P lactiflora either alone or in combination with one or more of the other 3 components, according to permutation and combination, were prepared and their benzoic acid contents determined by RP HPLC Results The content of benzoic acid in the decoction of Radix Paeonie Alba alone was found to be 2 74 mg/g, while that of the other 7 combinations varied from 0 41～1 11 mg/g Conclusion The content of benzoic acid in SIWUTANG could be lowered significantly by the presence of Radix Angelica Sinensis, Radix Rehmanniae, and Rhizoma Chuanxiong

OBJECTIVE:To further perfect our Pharmaceutical Administration Law of PRC.METHODS:Contractive methods were used to analyze comparatively the legal liabilities in US FDCA and Pharmaceutical Administration Law of PRC with regard to awarding system for reporters,penalty terms and the disposition of the confiscated drugs.RESULTS&CON?CLUSION:Compared with the PRC Pharmaceutical Administration Law,the US FDCA is more comprehensive and more consummate.We should follow the legislative spirit in FDCA and take it as a reference to improve our Pharmaceutical Ad?ministration Law.

OBJECTIVE:To provide references for the establishment of drug recall system in China.METHODS: The drug recall system in U.S.A. was introduced so as to get some enlightenment for the drug safety in China.RESULTS & CONCLUSION: We could use the drug recall system in U.S.A. for references to improve our law criterion system and carry out drug recall system on a large scale.

Invasive fungal infections(IFI) is significantly increasing in the neonatal intensive care unit recent years.The characteristics of IFI are low diagnosis rate,high mortality and being prone to involve in the nervous system.In this paper,the epidemiology,pathogens,risk factors,diagnosis,treatment and prevention of neonatal IFI are reviewed to improve the understanding of this disease.

Adult ; Histiocytosis, Langerhans-Cell ; Humans ; Male

Through the surveys on English majors and graduates,several proposals are put forward to optimize English major curriculum in Xinxiang Medical University. The training objectives are defined accordingly,centering on training qualified English language personnel.

This paper discuss new object about internal hospice care. first: Hospice care will suit for the needs of market economy. Second: Hospital Care how to neet different demoands.

Objective:To establish an HPLC method for the simultaneous determination of 7 flavonoids in Fengliaochangweikang capsules, and optimize the extraction process for total flavones. Methods:The extraction process was optimized by orthogonal test. The analysis was performed on a Waters RP18 column (250 mm × 4. 6 mm,5 μm). The mobile phase A was methanol-water (80 :20) and the mobile phase B was methanol-0. 2% formic acid (17 :83) with gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was 35℃. The detection wavelength changed as follows:0-27 min,λ1 = 260 nm;27-60min,λ2 = 360 nm. Results:The 7 flavonoids obtained the baseline separation in 60 min, and the linear correlation coefficients were all above or equal to 0. 9900. The average recovery was 95. 95%-97. 84% and RSD was 0. 81%-1. 33%. The optimum extraction conditions were as follows:solid-liquid ratio was 1 :15,AB-8 macroporous adsorption resin was used, the ultrasonic extraction time was 30min, and the ethanol concentration was 60%. Conclusion:The established method has the advantages of simple operation, high precision and good repeatability,which shows certain guiding significance for the quality control and detection of Fengliaochangweikang capsules.

OBJECTIVE:To study the interaction between bovine serum albumin(BSA)with lamivudine,efavirenz,tenofovir and its mechanism. METHODS:Fluorescence spectroscopy was used to determine the interaction between BSA with different con-centrations of lamivudine,efavirenz,tenofovir under different temperatures. The fluorescence intensity of them were determined re-spectively;quenching constant(KSV),apparent quenching constant(Kq),binding constant(KA),binding site(n),thermodynamic enthalpy change(ΔH),free energy diversification(ΔG)and entropy change(ΔS)were calculated according to Stern-Volmer equa-tion and so on. Molecular docking model of 3 drugs with BSA was established by using Sybyl 6.7 Flex X model. RESULTS:Kq for the interaction between 3 drugs with BSA were all higher than 2.0×1010 L/(mol·s),and were decreased with the increase of temper-ature;all n were close to 1,and thermodynamic functions ΔG<0,ΔS<0,ΔH<0. Molecular docking model showed that 3 drugs were mainly bound with BSA at Sudlow Ⅰ subdomain site. CONCLUSIONS:There are the interaction between 3 drugs with BSA;fluorescence quenching mainly manifests as static quenching;binding reaction belongs to spontaneous molecular action pro-cess;binding force mainly includes hydrogen bond and Van der Waals'force. The result of fluorescence experiment is consistent with those of molecular docking,and they complement each other.

Objective: To assess the expression of connective tissue growth factor (CTGF), and relevant mechanism for the regulation of CTGF expression by hypoxia in human renal interstitial fibroblast. Methods: A human renal interstitial fibroblast cell line TK173 was treated under hypoxia (1%O2) or nomoxia (21%O2) condition. The expressions of HIF1-?, hypoxia marker protein, and CTGF protein were analyzed by Western blotting. PT-PCR was carried out to measure the levels of CTGF mRNA. The activations of MAPKs (ERK, JNK, p38) signaling pathways were assessed at different time points (30 min, 1 h, 6 h, and 12 h ), and the changes of CTGF expression were detected after the inhibitors of activation of MAPKs were applied, respectively. Results: The expression of HIF-1? protein appeared in cells under hypoxia for 6 h. The expressions of CTGF protein were up-regulated in TK173 cells under hypoxia for 12 h, reached the peak levels in 2 folds of normoxia group cells for 24 h, and return to the levels of control cells by 48 h. The levels of CTGF mRNA were elevated in cells under hypoxia for 1 h, significantly increased at 6 h (6.6?1.0,P=0.000 2), and returned to the levels of normoxia group cells by 24 h. Activations of ERK1/2, JNK and p38 were seen in hypoxic cells. Activation of ERK1/2 and JNK were occurred as early as at 10 min, and reached the peak levels at 1 h, while the peak levels of activated JNK were seen at 30 min, then the levels of activated ERK1/2, p38, and JNK were all declined at 6 h, back to the baseline levels at 12 h. Blockade of ERK activation with PD98059, and blockade of JNK activation with SP600125 did not suppress hypoxia-induced expression of CTGF protein, whereas blockade of p38 MARK activation with SB203580 abolished hypoxia-induced expressions of CTGF protein and CTGF mRNA. Conclusion: Hypoxia could stimulate the expression of CTGF in human renal interstitial fibroblast through the activation of p38 MARK signaling pathway.