Type 2 diabetes is associated with a marked increased risk of cardiovascular disease (CVD).Treatment for dyslipidemia in diabetes reduces cardiovascular event.This article reviewed the clinical trials of lipid lowering in diabetic subjects,treatment goals for lipoprotein therapy,and how to choose lipid lowering agents.

0.05) and associated with histopathologic grade and clinical stages (P

Objective:To explore clinical significance of the further method dealt with in ASCUS(atypical squamous cells undetermined significance) of cervical smear.Methods:118 patients with ASCUS were detected by colposcopy and high risk HPV test,simultaneously make biopsy of cervical guided by colposcopy.Result:By diagnosed with pathology,chronic cervical inflammation is 72 cases,46 cases were CIN(cervical intraepithelial neoplasia).In this study 40 cases were positive in HPV test which 33 cases are CIN(?2=48.18,P0.05).Conclusion:There are high CIN csses in Women with ASCUS whose should be paid attention to and strengthen management.Colposcopy and high risk HPV test are efficiency method to ASCUS,especially high risk HPV test is positive that need detective by colposcopy.The combination of colposcopy and high risk HPV test can improve detective rate and accuracy of cervical intraepithelial neoplasia(CIN).

OBJECTIVE: To summarize research progress of tissue-engineered tendon stent material and its application for repairing tendon defect. METHODS: A computer-based online search was conducted with the key words of "tissue engineering, tendon, scaffold" in both Chinese and English from January 1993 to October 2009. Articles about dynamical performance of tissue-engineered tendon,experimental research and clinical application of tissue-engineered tendon were included, but duplicated studies and Meta analysis were excluded. Among 38 articles, 4 major viewpoints were discussed: research progress of tendon tissue engineering; biodynamical analysis of tissue-engineered tendon stent materials; application of biomaterials for tendon tissue engineering; clinical application of tissue engineering for repairing tendon defect. RESULTS: Natural polymer and synthetic macromolecule were major materials in tendon tissue engineering. The preparation and selection of stent materials depended on producing imitated materials with advanced performance and structure based on artificial synthetic materials, preparing natural derivative stent materials based on natural extracellular matrix, and constructing tissue-engineered tendon based on original bio-structure and bio-performance of biological tissue. Construction of tissue-engineered tendon must simulate three-dimensional physical environment in vivo. Microcomputer-controlled step motor was used to force tendon-matrix compound stent in order to increase proliferation velocity of tendon cells and secretory volume of collagen and develop into stress direction. CONCLUSION: Physical stimulation is simple, thus technique and instrument for constructing artificial tendon need to be further studied. Additionally, living implants which may completely replace body tissue and organ function still need to be further studied and solved.

ObjectiveTo explore the mechanisms of proliferation and apoptosis of K562 cells induced by cytarabine. MethodsDifferent doses of cytarabine on the proliferotion at K562 cell 24h,48h,72h were detected by MTT. Cell cycle And Apoptosis rate were detected by FCM. ResultsEach concentration of cytarabine obvious inhibited the growth of K562 cells. In the same concentrations,function of proliferation inhibition with cytarabine increased by the extension of time,which showed dose and time effect relations. Cytarabine on K562 cell,G0/G1 phase cell proportion of role as cytarabine increased when extension of time, S period and the G2/M period cells were decreased.Apoptosis of K562 cell in proportion as drug concentration and effect time increases, which present Cytarabine timeconcentration dependence. CondusionCytarabine was apparent inhibition to proliferation of K562 cell leukemia cell, which could promote K562 cell apoptosis by time and concentration dependence way.

BACKGROUND:Tumor stem cels have self-renewal, drug resistance and metastasis tumorigenicity, which play an important role in occurrence, development and metastasis of tumors. Currently, there are two methods to identify tumor stem cels, namely, in vitro tumor sphere culture experiments and in vivo mouse tumorigenic experiments. However, there ia a lack of reports regarding clinicaly enriched gastric cancer cels by chemotherapy. OBJECTIVE:To investigate the enrichment of rat gastric cancer stem cels by cisplatin, and to explore the screening methods for their surface marker proteins. METHODS: BCG-823 gastric cancer model was established in rats, and then rat models were randomized into two groups: rats in experimental groups were subjected to intravenous injection of 0.1, 0.2, 0.25, 0.3 g/L cisplatin via the tail vein; those in control group were injected with normal salinevia the tail vein. After three courses of chemotherapy, gastric stem cels-enriched tissues were colected. Tumor surface proteins were extracted using high-throughput protein microarray and identified by western blot assay. Effects of cisplatin on enrichment of rat gastric cancer stem cels and screening methods for surface marker proteins were compared. RESULTS AND CONCLUSION:Cisplatin at a dose of 0.3 g/L×200μL exhibited the best therapeutic effects, and moreover, with the dose increasing, the tolerance became worse and the incidence of adverse reaction became higher. Transplantation tumors were verified by hematoxylin-eosin staining. Western blot test results were similar to the findings of protein microarray method, that is, HLA-DQ, PMP22 and Claudin7 protein expressions increased in gastric tissues, but HLA-DR, CD14, CD16 and CD56 protein expression decreased. These findings suggest that cisplatin can be used to enrich gastric cancer stem cels in rats, and to successfuly screen the corresponding surface marker proteins.

Objective To explore the application value of 256 slice helical multi phase CT scan-ning and three-dimensional reconstruction in the diagnosis of malignant tumor of colorectal obstruction. Methods Using 256 slice spiral CT scanning and three-dimensional reconstruction of multi period in 42 ca-ses of malignant tumor patients with colorectal obstruction, the results and pathological results were analyzed and the postoperative stage.Results Forty-two cases of malignant tumors of colorectal obstruction, 256 slice spiral CT could well reflect the situation of location, range, degree, peripheral intestinal lymph node and distant metastasis, CTA could show the tumor supplying artery and branch sources, on the tumor loca-tion and overall accuracy.Conclusions 256 layer spiral CT scan and three dimensional reconstruction technology is accuracy for clinical diagnosis on malignant tumor of colorectal obstruction.

Heterogeneous nuclear ribonucleoprotein (hnRNP) is a family of multifunctional nuclear RNA-binding proteins that regulate the alternative splicing of pre-mRNA and the transport, translation, and stability of mRNA. The most abundant and best charac-terized proteins of this group are hnRNP A1 and hnRNP A2, which share a high degree of sequence homology and functional similarity. HnRNP A1 and hnRNP A2 are upregulated in multiple human tumors and modulate the alternative splicing and mRNA stability of vari-ous tumor-related genes critical to tumor cell growth, apoptosis, inflammatory and immune reactions, and epithelial-to-mesenchymal transition. Therefore, hnRNP A1 and hnRNP A2 have potential diagnostic, prognostic, and therapeutic values.

Objective To investigate the effect of maternal serum vitamin D (VD)levels on the developing nervous system in in-fants.Methods A total of 1 012 pregnant women's serum VD levels of early pregnancy were given the right amount of VD supple-mentation according to the results.They were divided into 3 groups (normal,deficiency and insufficiency group)according to the third trimester VD levels;and their infants 6-8 months were tested on the developmental quotient (DQ),mental index (MI)and other neurological development index by the Developmental Screening Test for child under six(DST).Results There were up to 94.17% of pregnant women in early pregnancy had severe VD deficiency,the average levels was (16.21 ± 7.54)ng/mL;Although VD levels was increased after supplements,there were 46.25% of pregnant women still deficient in VD.The average DQ score of 1 020 infants was 92.67±16.45 and M1 score was 97.53±17.04,which accorded with normal distribution.The rate of the infants born by mothers who had the normal VD levels were superior to those with VD deficiency and insufficiency on the quotients of out-standing and smart,and there was a significant difference between them(P <0.01).But there was no statistical difference between the deficiency and insufficiency group (P >0.05).The level of VD was positively correlated with DQ and MI,and the correlation coefficients are 0.63 and 0.2(slightly correlated),respectively.Conclusion It should be pay more attention to monitoring the VD levels of the pregnant women during the pregnancy,and for lack should be promptly added.

Objective The aim of this study was to examine hope level in patients with coronary heart disease after per-cutaneous coronary intervention and explore correlation with illness perception.Methods 176 patients in outpatient department were recruited after per-cutaneous coronary intervention.The Herth Hope Index (HHI),Illness perception Questionnaire-Revised and demographic and clinical characteristics were collected.Results The hope level score of patients after per-cutaneous coronary intervention was (36.18±3.21),the hope level was correlated with seven dimensions of illness perception and was influenced by degree of education,income level,whether the disease is acute or chronic and control belief.Conclusions The patients after per-cutaneous coronary intervention show high level of hope and is affected by many factors,this provides the basis for taking effective measures to improve the patients' level of hope.