Chitinases (designated as SPCs) were isolated from „Shilbilati‟ potatoes, a potato prototype cultivated in Bangladesh by affinity chromatography on a chitin column. SPCs agglutinated rat erythrocytes at the minimum concentration of 7 μg/mL and showed toxicity against brine shrimp nauplii with the LC50 value of 20 μg/mL. The chitinases also agglutinated seven bacterial strains among the twelve as studied. Pseudomonas aeruginosa, Bacillus subtilis and Salmonella typhi were the most sensitive towards the SPCs and were agglutinated at 1.2, 2.5 and 5.0 μg/mL protein concentrations respectively. Antibacterial tests demonstrated that SPCs showed inhibitory activity against the pathogenic bacteria Staphylococcus aureus, Bacillus subtilis and Salmonella typhi. Antifungal activity was investigated by the disc diffusion method. Five fungal species (Candida albicans, Aspergillus niger, Fusarium vasinfectum, Aspergillus fumigatus and Aspergillus flavus) and two fungal genus (Penicillium and Mucor sp.) were examined in the assay. SPCs showed antifungal activity against Candida albicans, Fusarium vasinfectum and Penicillium sp.

Introduction: Vitamin A deficiency is one of the most serious health problems in developing countries. This study aims to explore the comparative figure of vitamin A knowledge, household consumption frequency of vitamin A-rich foods, and association of socio-demographic factors with knowledge and consumption in four residential areas in Tangail district, Bangladesh. Methods: This study used a population-based cross-sectional design with 400 study participants selected using purposive sampling technique. Knowledge and consumption frequency were assessed by a structured questionnaire along with food frequency table. Data were analysed using descriptive statistics. Results: Only 33.5% participants had primary vitamin A knowledge, where most from urban (48.5%) and semi-urban (30.6%) areas had comparatively higher knowledge than those from rural (11.2%) and slum (9.7%) areas. Specific knowledge level was also poorer in rural and slum areas than urban and semi-urban areas. Most of the participants received knowledge through commercial advertisements (65.7%). Household consumption frequency of vitamin A-rich foods (plant and animal) was comparatively lower in rural and slum areas than in urban and semi-urban areas. Different socio-demographic factors (place of residence, education and household income) significantly influenced participant’s vitamin A knowledge and household consumption of vitamin A-rich foods (p<0.05). Conclusion: In general, the study population lacked knowledge regarding the importance of vitamin A. Consumption frequency of vitamin A-rich foods was still poorer in slum and rural areas than in urban and semi-urban areas.