Objective To analyzer the clinical significance of the Xpert MTB/RIF method for rapidly detecting Mycobacterium tuberculosis(MTB) and rifampicin resistance in clinic.Methods A total of 552 sputum samples were collected from the patients in the Huizhou Municipal Institute of Tuberculosis Prevention and Control and tuberculosis departments in 5 counties and districts from January 2015 to December 2015.Smear microscopic examination ,Xpert MTB/RIF nucleic acid detection and improved Roche culture were performed.The samples of culture positive were performed the drug susceptibility test.The sensitivity ,specificity of various methods for detecting MTB were evaluated.The results of conventional susceptibility test and Xpert MTB/RIF for detec-ting rifampicin resistance were compared and analyzed.Results Compared with the Roche culture method ,the sensitivity and speci-ficity of Xpert MTB/RIF for detecting MTB were 95.3% and 93.1%.In the comparison of drug resistance detection results ,the sensitivity and specificity of rifampicin resistance in all positive samples were 82.1% and 97.8%.In the consistency analysis of Xpert MTB/RIF and improved Roche culture for detecting MTB classification ,kappa=0.688 ,indicating that the results of the two methods had good consistency.Conclusion The Xpert MTB/RIF method can be used in MTB early rapid detection and screening of rifampicin resistance detection ,which is conducive to clinical rapid decision.

Objective To investigate the effect and mechanism of osteopontin(OPN)in hepatoma cell migration through galectin-3 binding protein(LGALS3BP).Methods Human hepatoma cell lines SMMC-7721,SMMC-P(stably transfected with empty eukaryotic expression vectors),and SMMC-OPN(stably transfected with the OPN gene)were cultured.mRNA expression levels of OPN and LGALS3BP were measured by RT-qPCR.Western blot assays were used to analyze the relative protein expression of OPN and LGALS3BP and PI3K/AKT pathway.Wound healing assays were performed to explore the cell migration ability.After transfection with LGALS3BP-targeting small interfering RNA(si-LGALS3BP)or negative control small RNA(si-NC)into SMMC-OPN cells,cell migration and relative expression of PI3K/AKT pathway-related proteins were assessed.Results Compared with SMMC-7721 and SMMC-P,the migratory ability of SMMC-OPN cells was significantly reinforced,and expression of LGALS3BP was obviously upregulated at both mRNA and protein levels.Moreover,relative expression of p-PI3K/PI3K and p-AKT/AKT proteins was significantly increased.Wound healing assays showed that the si-LGALS3BP obviously suppressed the migratory ability of SMMC-OPN cells.Furthermore,relative expression of p-PI3K/PI3K and p-AKT/AKT proteins in SMMC-OPN cells was significantly decreased after transfection of si-LGALS3BP.Conclusions OPN activates the PI3K/AKT pathway by upregulating LGALS3BP expression to promote hepatoma cell migration.