Objective:To investigate the influence of compound glycyrrhizin on expression of HMB45,TNF-? and IL-6 in lesion of compound. using vitiligo guinea pig model induced by chemical decolorized compound.Methods:Two Guinea pig model groups were respectively treated with compound glycyrrhizin in 27.8 mg/kg and 55.6 mg/kg dosage. Immunohistochemical studies were performed to study the expression of HMB45,TNF-? and IL-6 in compound glycyrrhizin treated group,vitiligo model group and normal control group.Results:The expression of HMB45 in lesion of vitiligo model group was lower than the normal control group.The expression of HMB45 in lesion of compound treated group was higher than the vitiligo model group and the expression of HMB45 increased accompany by the increase of dosage.The expression of TNF-? and IL-6 in vitiligo model group was significantly higher than the normal control group (P

Objective To investigate the rate of vaginal colonization of Candida species in patients with diabetes,and to discuss the relationship between prevalence of vulvovaginal candidiasis(VVC) and diabetes mellitus(DM).Methods Genital tract examination and fungal cultures of discharge taken among 144 patients with DM(DM group) and 150 healthy subjects(control group) were performed.Chrom Agar Candida mediums were used to study the isolates of 64 strains cultured in 144 participants.Results The isolated rates of Candida species were 44.4%(64/144) in subjects with DM and 18%(27/150)in healthy subjects,the isolated rate of Candida species in DM group was prominently higher than that in control group(P

Objective To investigate the therapeutic effect of glycyrrhiza active substance on guinea pig model of vitiligo,and to clarify its mechanism on promoting synthesis of melanin,and to provide the basis for its clinical application.Methods Guinea pigs decolorized by H2O2 were used as experimental models,and normal control group was set up.After treatment of glycyrrhiza active substance in three doses groups(20,40,and 80 mg?kg-1),the therapeutic effect was observed by visual observation,macrography,histological examination as well as immunohistochemical study.Results The significant differences of skin color among control group,model group and treatment groups were observed by macrography.The total effective rates in 40 or 80 mg?kg-1 groups were higher than that in control group(P

AIM: To study inhibitory effect on proliferation of docetaxel on murine angiosarcoma cell line (ISOS-1) and compare with etoposide. METH ODS: In vitro, the inhibitory effect on proliferation of docetaxel and etoposide on ISOS-1 cells were carried out by Alamar Blue assay. In vivo study, after murine angiosarcoma model establishment, seventy mice were divided into trial and control group with 5 mice for each group. The dosages of docetaxel and etoposide were 5, 10, 20 mg · kg-1 respectively through administration by intravenous (iv) or intraperitoneal (ip) injection. The iv injection was performed once a week and total 4 times as one course. The ip injection was taken once a day, keeping for 5 d as a course and then repeated once more after 2 wk. The control group was injected with the normal saline. The volumes of the tumors and the survival days were calculated. RESULTS: In vitro study, the IC5o of docetaxel for ISOS-1 cells was 15.8 μg · L-1 showing obviously lower than that of et oposide group ( 1. 175 mg · L-1 ). In vivo study, the anti-tumor effect of docetaxel was better than that of et oposide at three different doses by iv, and all the in hibitory rates of tumor volume were more than 70 %. The life prolonging effect of 5 mg · kg- 1 docetaxel was similar to that of etoposide 10 mg · kg-1. The adverse reactions of ip injection were stronger than those of ivinjection. The dosage of etoposide 20 mg · kg-1 almost reached the lethal dose. CONCLUSION: Docetaxel shows obviously inhibitory effects on proliferation of murine angiosarcoma cell line (ISOS-1), which is superior to that of etoposide. It is safe and effective with low dosage once a week by iv.

Objective:To compare the efficacy and adverse reactions between intralesional betamethasone injection combined with botulinum toxin type A and topical hyaluronic acid and simple intralesional betamethasone injection and topical hyaluronic acid in treatment of keloid.Methods:A total of 58 patients with keloid were divided into two groups: intralesional betamethasone injection combined with botulinum toxin type A and topical hyaluronic acid group (combined group, n=28) and intralesional betamethasone injection combined with topical hyaluronic acid group (control group, n=30).In both two groups, the intralesional betamethasone injection was given once every 4 weeks for 3 times totally, and topical hyaluronic acid was applied every day.In combined group, an injection of botulinum toxin type A was given around the lesion after the first intralesional injection of betamethasone.The parameters for therapeutic efficacy and adverse reactions of the patients in two groups were recorded, and the clinical pictures were taken before and after each treatment, and they were analyzed and compared.Results:After 3 treatments, compared with control group, the lesion appearance in combined group was improved.In control group, the VAS value was reduced at 1 month, but was increased gradually at 2 and 3 months. In combined group, the VAS value was reduced gradually in 3 months. There was a significant difference in VAS value at 2 and 3 months between two groups (P<0.05).In combined group, the thickness of keloids were reduced gradually in 3 months, while the reduction was not obvious in control group (P<0.05).The recurrence of pain, itching and lesion height were observed 2 weeks after each treatment in control group, but there was no recurrence in combined group.The incidence rate of adverse reactions was 26.7% in control group, which had no significant difference compared with combined group(25.0%) (P>0.05).Conclusion:Compared with control group, the combined group shows better efficiency in the treatment of keloid and there is no increase of adverse reactions.The therapy is worthy of clinical application.

Objective:To identify the HLA-DRB alleles associated with vitiligo and psoriasis in Chinese Han people of Jilin region.Methods:HLA-DRB alleles of 82 vitiligo patients and 80 psoriasis vulgaris patients were detected by polymerase chain reaction sequence-specific primer(PCR-SSP),under the comparison with 86 healthy persons.Results:①The frequence of HLA-DRB1* 07 and HLA-DRB1*12 alleles increased obviously in vitiligo group compared with control group(P

Objective:To study whether gangliosides inhibit the antigen-presenting capability of epidermal Langerhans cells.Methods:In the vitro test, the purified Langerhans cells were exposed to increasing concentration of gangliosides for 5 hours at 37℃,then KLH was added and incubated for 2 hours at 37℃.At last we added HDK1 and after 72 hours of coculture, levels of IFN-? in culture supernatants were measured by ELISA. In the vivo test, immunity to the S1509a spindle cell carcinoma was induced by s.c. inoculation at weekly intervals into naive syngeneic(CAF1) for a total of three immunizations. Delayed-type hypersensitivity(DTH) was elicited 1 week after the last immunization by injection a hind footpad with TAA-pulsed Langerhans cells incubated with or without gangliosides. Footpad swelling was assessed at 24 and 48 hours with a spring-loaded engineer′s micrometer.Results:The presence of gangliosides during HDK1 activation reduced the expression of IFN-? in vitro test. Gangliosides suppressed Langerhans cells to elicit DTH against TAA in vivo test.Conclusion:Ganglioside inhibit the antigen-presenting capability of epidermal Langerhans cells.

Objective:To investigate the efficacy and safety of photodynamic therapy (PDT) combined with oral isotretinoin in the treatment of moderate to severe acne.Methods:Ninty patients with moderate and severe acne [42 males and 48 females; aged 18-35 (23.98±4.25)] in the Department of Dermatology, China Japan Friendship Hospital of Jilin University from January to June, 2018, were divided into three groups: PDT combined with isotretinoin group (combined group), PDT group and isotretinoin group, with 30 patients in each group. The curative effect was evaluated on the 30th and 60th day of the treatment and the follow-up was carried out regularly.Results:At 10, 20, and 30 days of the treatment, the clearance rates of non-inflammatory skin lesions and total skin lesions were better than that of the PDT group; the clearance rates of inflammatory, non-inflammatory and total skin lesions in the combined group were better than those of the isotretinoin group. At 30 days of treatment, the efficacy of the combined group was better than that of the PDT group (χ 2=13.169, P<0.05) and isotretinoin group (χ 2=19.606, P<0.05). The treatment course of the combined group was shorter than that of the PDT group ( P<0.05). Six months after the treatment, the recurrence rate of the combined group was lower than that of the isotretinoin group (χ 2=4.941, P<0.05). And there was no significant difference of the incidence of adverse reactions between the above two groups ( P>0.05). Conclusions:The combination of photodynamic therapy and oral isotretinoin therapy for moderate to severe acne can improve the efficacy, shorten the course of treatment, and reduce the recurrence rate and adverse reactions, which provides a new idea for the treatment of acne.