Neural system diseases are the significant burden threatening life quality of human beings. The investigation of stem cells especially the adult stem cells improved the related basic and clinical research of biological therapy and highlighted a prpspective future. The transplantation of adult stem cells maybe an effective method to treat neural system diseases. So the collaboration between the basic and clinical research will be strenthened to serve patients. Translational medicine is a potential field in which the basic medicine and clinical medicine and linked.

The current therapeutic strategies for ischemic stroke are more limited. In the field of biotherapy bone marrow mesenchymal stem cell(BMSC) is a seed cell with the most potential. Synthesizing the advances in methodology of preclinical studies, this article mainly reviews the types of BMSC, time window for cell transplantation, approaches of cell transplantation, tracing technique in vivo after cell transplantation, and treatment effectiveness evaluation.

Objective To investigate the expression of c-erbB-2 in breast cancer and its relationship with prognosis.Methods The expression of c-erbB-2 in breast cancer was detected by immunohistochemistry assays in 60 cases of breast cancer.Results The positive expression level of c-erbB-2 in invasive breast cancer was signifi-cantly higher than that in other kinds of breast cancer(P<0.01).The positive expression level of c-erbB-2 in the metastatic breast cancer with four lymph nodes was higher than in the early cancer with no lymph node involvement (P<0.01).The 10 years survival rate in negative expression of c-erbB-2 was higher than in positive expression(P<0.05).Conclusion c-erbB-2 is important for physicians to instruct the treatment of the breast cancer and judge the prognosis.

Objective To investigate the expression of c-erbB-2 in breast cancer and its relationship with prognosis.Methods The expression of c-erbB-2 in breast cancer was detected by immunohistochemistry assays in 60 cases of breast cancer.Results The positive expression level of c-erbB-2 in invasive breast cancer was signifi-cantly higher than that in other kinds of breast cancer(P<0.01).The positive expression level of c-erbB-2 in the metastatic breast cancer with four lymph nodes was higher than in the early cancer with no lymph node involvement (P<0.01).The 10 years survival rate in negative expression of c-erbB-2 was higher than in positive expression(P<0.05).Conclusion c-erbB-2 is important for physicians to instruct the treatment of the breast cancer and judge the prognosis.

The neural stem cells (NSCs) can migrate into the injured area and differentiate into neurons or oligodendrocytes.Endogenous neurogenesis may potentially be harnessed as a putative therapy for neural injury.But the complex micro-environment due to TBI will be one of the biggest challenges for endogenous NSCs to perform neural regenerations.Exogenous NSCs have been shown to be able to survive in host tissues and regulate microenvironment via paracrine effects.Thus, transplantation of NSCs to assist neural regeneration has become an attractive option.Recently, rapid advances in the stem cell biology have raised appealing possibilities of replacing damaged or lost neural cells by transplantation of in vitro-expanded stem cells and/or their neuronal progeny.

Objective:To explore the expressions of the nuclear factor-?B(NF-?B),C- myc and ICAM-1 in human colon and rectal cancer and the relationship between NF-?B and the occurrence,the metastasis of human colon and rectal cancer.The clinical significance was analyzed.Methods:Fifty human colon and rectal cancer cases ,16 colon and rectal adenomas,9 carcinomatous change of colon and rectal adenoma and 8 normal colon were studied,the expressions of NF-? Bp65,C- myc and ICAM-1 in them were examined by immunohistochemical method.Results:NF-?Bp65,C- myc were strongly expressed in the colon and rectal cancer and carcinomatous change of colon and rectal adenomas than those in colon and retal adenoma;C- myc could not express in normal colon;NF-? Bp65 and C- myc were significantly positive correlation with colon and rectal cancer;NF-?Bp65,ICAM-1 were strongly expressed in the colon and rectal cancer which had metastasis than those had no metastasis,and they had striking positive correlation with colon and rectal cancer which had metastasis.ConclusionTo control the transcription of C- myc and ICAM-1,NF-?B plays an important action in the occurrence and metastasis of colon and rectal cancer.It will become a new target of treatment of colon and rectal cancer.

Purpose:To investigate the expression of nuclear factor kappa binding (NF ?Bp65),c myc and intercellular adhesion molecule 1(ICAM 1) in colorectal cancer and its relation to clinicopathology.Methods:50 colorectal cancer patients who underwent radical surgery were studied, the expression of NF ?Bp65, c myc and ICAM 1 in them were examined by immunohistochemical method.Results:①In normal colon there was weak expression of NF ?Bp65, no expression of c myc and ICAM 1, NF kBp65,C myc and ICAM 1 were strongly expressed in colorectal cancer than in normal colon( P

Objective To investigate the expression of eukaryotic initiation factor 4E(eIF4E) ,vascular endothelial growth factor (VEGF)‐A and VEGF‐C in gastric cancer tissues and their correlation with invasion and metastasis of gastric carcinoma .Methods The expressions of eIF4E ,VEGF‐A and VEGF‐C were detected in tissues of 58 gastric carcinomas and 25 normal gastric mucosa by using immunohistochemical method .Results The positive rate of eIF4E、VEGF‐A and VEGF‐C protein expression were 89 .7%(52/58) ,65 .5% (38/58) ,60 .3% (35/58) in gastric carcinoma ,which were higher than those in normal gastric mucosa tissues which were 4 .0% (1/25) ,12 .0% (3/25) ,8 .0% (2/25) respectively .Expressions of eIF4E ,VEGF‐A and VEGF‐C were significantly correlated with the depth of invasion and lymph node metastasis(P<0 .05) ,but not with the age ,sex of patients(P>0 .05) .Ex‐pressions of eIF4E and VEGF‐C were significantly correlated with tumor differentiation .The expression of eIF4E was being found positively correlated with VEGF‐A and VEGF‐C .Conclusion eIF4E may play certain roles in the oncogenesis and progression of gastric carcinoma .VEGF‐A and VEGF‐C may be helpful in lymph metastasis .Combined detection of eIF4E ,VEGF‐A and VEGF‐C may be helpful to assess the malignant degree and prognosis of gastric carcinoma .

Objective To explore the value of MRI and iron oxide particle labeling in stem cell therapy of stroke model. Methods Nine rats with middle cerebral artery occlusion were randomly selected and underwent Neurological Severity Scoring(NSS), MRI and pathological examination. The results of the 3 evaluation criteria were correlated. Bone marrow stromal cells were labeled with superparamagnetic iron oxide particles. Eighteen models were screened and divided into 3 groups based on different transplantation sites. MRI was performed at different time points. The MR appearance of labeled stem cell transplantation sites was observed. The relative infarct volume of the models in three groups were recorded and compared. Results Significant correlations among the NSS, MRI and pathological examination were found. Different MR sequences could depict local transplanted labeled stem cells and gradient echo sequence was the most sensitive method, while the T2WI showed its advantage of better temporal resolution. MR images showed the morphological changes of transplanted stem cells. The change of the relative infarct volume showed no significant differences among the three groups. Conclusion MRI is an ideal tool to evaluate the rat stroke model. MRI together with iron oxide particle labeling technique helps to in vivo track and monitor the transplanted stem cells.

Objective To label Flk-1+CD31-CD34-human mesenchymal stem cells (hMSCs) with superparamagnetic iron oxide (SPIO) and to evaluate the effect of SPIO on proliferation and neural differentiation of labeled cells. Methods hMSCs were incubated with SPIO (50 mg/L) and PLL (1.5 mg/L) overnight(12～18 hours). Both labeled and unlabeled cells went through growth curve test,Trypan blue staining and flow cytometer to evaluate the effects of SPIO on cell proliferation,cell viability and surface markers. Immunofluorescence assay was conducted for neuron and neuroglia specific cell surface markers after neural induction protocols were used. Results Cell viability of the two groups were both more than 90% for 7 days. There was no significant difference in cell viability and growth curve test between two groups. The results of flow cytometer showed that both labeled and unlabeled cells expressed CD44, CD105 and Flk-1 markers, while CD31 and CD34 were negative. After neural induction, the statistical analysis of A value for all the markers showed no significant difference between the two groups.Conclusion SPIO, as MRI cellular contrast, is safe and efficient.