Objective To elevate the diagnosis value of spiral CT in blunt duodenum trauma. Methods 9 cases of blunt duodenum trauma proved by operation or clinical were analysed retrospectively. Results 9 cases of blunt duodenum trauma were all diagnosed accurately before operation. The main CT signs were duodenal wall thick- ening; duodenal wall hematoma; free retroperitonea air; free retroperitouea fluid and retoperitonea hematoma. Conclu- sion Spiral CT scan have important clinical value in diagnosis of blunt duodenum trauma.

Objective To study the expression level and clinical significance of Galectin-3 and miRNA-21 in non-small-cell lung carcinoma(NSCLC).Methods One hundred and fifty patients with NSCLC were chosen as cancer group,and 1 50 patients with benign pulmonary diseases were chosen as control group.The expression level of Galectin-3 and that of miRNA-21 between two groups were compared,and the relevance between expression level of Galectin-3 and that of miRNA-21 and clinical feature were analysed.Results In cancer group,the expression level of Galectin-3 was 6.75±2.38,and that of control group was 1.12 ±0.29;the expression level of miRNA-21 was 5.91 ± 1.59,and that of control group was 0.97 ± 0.1 7,and the difference between two groups had statistical significance(P <0.05 ).The relevance between expression level of Galectin-3 and stage,differentiation,lym-phatic metastasis,diameter of carcinoma and PFS,OS of patients had statistical significance(P <0.05).The relevance between ex-pression level of miRNA-21 and stage,differentiation,diameter of carcinoma and PFS,OS of patients had statistical significance(P <0.05).In the diagnosis of NSCLC,the sensitivity of the expression level of Galectin-3 was 90.20%,and its specificity was 70.69%, while the sensitivity of expression level of miRNA-21 was 88.24% and its specificity was 69.97%.The difference between the di-agnostic value of Galectin-3 and that of miRNA-21 had no statistical significance(P >0.05 ).Conclusion The expression level of Galectin-3 and that of miRNA-21 can be applied in the diagnosis and prognosis of non-small-cell lung carcinoma.

Asthma is one of the chronic diseases with the highest incidence in childhood. At present, comprehensive treatments such as glucocorticoid inhalation and bronchodilators can effectively control asthma. Parents' compliance with asthma treatment is closely related to the prognosis of children. This article reviews the influencing factors of treatment compliance and nursing strategies in children with asthma, aiming to provide a theoretical basis for effective control and management of childhood asthma in the future.

Objective:To establish and validate a reliable and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the detection of 12 ceramides in human plasma.Methods:From October 2021 to October 2022, 438 apparently healthy individuals were enrolled in the Affiliated Hospitals of Zunyi Medical University for reference intervals of 12 ceramides in this population. Plasma samples were collected, and separated using the ACQUITY UPLC BEH C18 (2.1×50 mm, 1.7 μm) column, deuterated isotopes were used as internal standards. The mobile phase is water (containing 0.1% formic acid) and isopropanol: acetonitrile (1∶1, v/v, containing 0.1% formic acid) at a flow rate of 0.4 ml/min with gradient elution. The detection method was established using the Qlife Lab 9000 Plus triple quadrupole mass spectrometer. The performance of the method was evaluated in terms of linearity, the lower limit of quantification, precision, recovery, and stability.Results:The method passed the performance evaluation in terms of linearity, the lower limit of quantification, recovery, precision, and stability. The intra-and inter-batch precision of the 12 ceramides ranged from 1.3% to 14.3%, the correctness was verified by spiked recovery experiments, and the recoveries ranged from 91.9% to 111.0%. The lower limit of quantification ranged from 0.001 to 0.100 μmol/L. Standard curve showed good linearity (correlation coefficient r>0.990). Stability tests showed that the 12 ceramides were stable in the biological matrix and after processing under different conditions for a specified period of time. The corresponding biological reference intervals were established for each of the 12 ceramides: 0.103-0.326 μmol/L for Cer(d18∶1/16∶0), 0.018-0.098 μmol/L for Cer(d18∶1/18∶0), 0.933-3.919 μmol/L for Cer(d18∶1/24∶0), 0.243-1.072 μmol/L for Cer(d18∶1/24∶1), 0.001-0.007 μmol/L for Cer(d18∶1/14∶0), 0.022-0.095 μmol/L for Cer(d18∶1/20∶0), 0.185-0.835 μmol/L for Cer(d18∶1/22∶0), 0.003-0.022 μmol/L for Cer(d18∶0/16∶0), 0.001-0.016 μmol/L for Cer(d18∶0/18∶0), 0.017-0.156 μmol/L for Cer(d18∶0/24∶0), 0.008-0.074 μmol/L for Cer(d18∶0/24∶1), and 0.106-0.721 μmol/L for LacCer(d18∶1/24∶1). Conclusion:Our study shows that the newly established LC-MS/MS method for the determination of 12 ceramides in human plasma is reliable, and suitable for clinical application.

Objective:To establish a practical and systematic pre-deployment base-based training course for medical units in peacekeeping level-II hospitals.Methods:We surveyed the current status of pre-deployment training of medical teams in peacekeeping level-II hospitals, and organized expert discussions to preliminarily construct centralized training course contents. At the same time, the Delphi method was used to conduct two rounds of questionnaire-based consultation to evaluate the overall framework design of the course and the rationality and importance scores of each module. The contents of the pre-deployment training course were further selected and optimized based on the positive coefficient (%), authority coefficient (Cr), coefficient of concordance ( W), and coefficient of variation (Cv). Results:The contents of the pre-deployment training course for the medical units of peacekeeping level-II hospitals were preliminarily established. The experts scored high for the combat onsite first-aid skills module [rationality score, (9.32±0.75) points; importance score, (9.45±0.65) points] and the combat wound treatment module [rationality score, (9.33±0.75) points; importance score, (9.28±0.74) points], and scored lowest for the field survival module [rationality score, (7.95±2.28) points; importance score, (7.87±2.16) points].Conclusions:The established contents of the pre-deployment base-based training course for the medical units of peacekeeping level-II hospitals are reasonable and practical, which lays the foundation for perfecting the pre-deployment training system of peacekeeping medical teams.

OBJECTIVETo analyze the polio immunity level of persistent population in Beijing, 2012.

METHODSA total of 1 676 subjects residing more than 6 months in Beijing were selected by stratified random cluster sampling design in 2012. Demographic characteristics, history of oral poliovirus vaccine (OPV) immunization were investigated by questionnaire. All 5 ml blood sample were collected for testing of polio neutralizing antibody using the method of microcell neutralization. The positive rate and the geometric mean titer (GMT) of polio neutralizing antibody type I, II and III were analyzed in different groups.

RESULTSThe positive rate of type I, II and III were 98.2% (1 645/1 676), 98.1% (1 644/1 676), 97.6% (1 635/1 676); The GMT were 1:130.2, 1: 113.4 and 1: 79.7. Three types of positive rates in<15 years group (99.7% (664/666), 99.8% (665/666), 99.5% (663/666)) were higher than those of ≥ 15 years group (97.1% (981/1 010), 96.9% (979/1 010), 96.2% (972/1 010)), the differences were significant (all the values of P < 0.01); The GMT in<15 years group (1:325.9, 1:250.5, 1:190.7) were higher than that of ≥ 15 years group (1: 71.1, 1: 67.2, 1: 44.8), the difference was significant (all the values of P < 0.01). The positive rate (99.0%-100%) and GMT (1: 128.8-1: 300.7) in vaccination information confirmed population were higher. The highest positive rate (all were 100%) and GMT(1: 409.7-1: 636.7) were observed in children who vaccinated three times.

CONCLUSIONThe polio antibody of healthy population was at a high level in Beijing in 2012; Especially the age groups of < 15 years which were covered by vaccines.Immunization barrier had been formed firmly to interrupt the transmission of wild poliovirus and vaccine-derived poliovirus.

Adaptive Immunity ; Adolescent ; Antibodies, Neutralizing ; Antibodies, Viral ; Child ; Humans ; Poliomyelitis ; Poliovirus ; Poliovirus Vaccine, Oral ; Vaccination ; statistics & numerical data

Objective To analyze the polio immunity level of persistent population in Beijing , 2012.Methods A total of 1 676 subjects residing more than 6 months in Beijing were selected by stratified random cluster sampling design in 2012.Demographic characteristics , history of oral poliovirus vaccine ( OPV) immunization were investigated by questionnaire.All 5 ml blood sample were collected for testing of polio neutralizing antibody using the method of microcell neutralization.The positive rate and the geometric mean titer ( GMT ) of polio neutralizing antibody type Ⅰ,Ⅱ and Ⅲ were analyzed in different groups.Results The positive rate of type Ⅰ,Ⅱ and Ⅲ were 98.2%( 1 645/1 676 ) , 98.1%( 1 644/1 676),97.6%(1 635/1 676);The GMT were 1∶130.2,1∶113.4 and 1∶79.7.Three types of positive rates in<15 years group(99.7%(664/666),99.8%(665/666),99.5%(663/666)) were higher than those of≥15 years group (97.1%(981/1 010),96.9%(979/1 010),96.2%(972/1 010)),the differences were significant(all the values of P<0.01); The GMT in<15 years group(1∶325.9,1∶250.5,1∶190.7)were higher than that of ≥15 years group (1∶71.1,1∶67.2,1∶44.8),the difference was significant (all the values of P<0.01).The positive rate(99.0%-100%) and GMT(1∶128.8-1∶300.7) in vaccination information confirmed population were higher.The highest positive rate (all were 100%) and GMT(1∶409.7-1∶636.7) were observed in children who vaccinated three times.Conclusion The polio antibody of healthy population was at a high level in Beijing in 2012; Especially the age groups of <15 years which were covered by vaccines.Immunization barrier had been formed firmly to interrupt the transmission of wild poliovirus and vaccine-derived poliovirus.

Objective To analyze the polio immunity level of persistent population in Beijing , 2012.Methods A total of 1 676 subjects residing more than 6 months in Beijing were selected by stratified random cluster sampling design in 2012.Demographic characteristics , history of oral poliovirus vaccine ( OPV) immunization were investigated by questionnaire.All 5 ml blood sample were collected for testing of polio neutralizing antibody using the method of microcell neutralization.The positive rate and the geometric mean titer ( GMT ) of polio neutralizing antibody type Ⅰ,Ⅱ and Ⅲ were analyzed in different groups.Results The positive rate of type Ⅰ,Ⅱ and Ⅲ were 98.2%( 1 645/1 676 ) , 98.1%( 1 644/1 676),97.6%(1 635/1 676);The GMT were 1∶130.2,1∶113.4 and 1∶79.7.Three types of positive rates in<15 years group(99.7%(664/666),99.8%(665/666),99.5%(663/666)) were higher than those of≥15 years group (97.1%(981/1 010),96.9%(979/1 010),96.2%(972/1 010)),the differences were significant(all the values of P<0.01); The GMT in<15 years group(1∶325.9,1∶250.5,1∶190.7)were higher than that of ≥15 years group (1∶71.1,1∶67.2,1∶44.8),the difference was significant (all the values of P<0.01).The positive rate(99.0%-100%) and GMT(1∶128.8-1∶300.7) in vaccination information confirmed population were higher.The highest positive rate (all were 100%) and GMT(1∶409.7-1∶636.7) were observed in children who vaccinated three times.Conclusion The polio antibody of healthy population was at a high level in Beijing in 2012; Especially the age groups of <15 years which were covered by vaccines.Immunization barrier had been formed firmly to interrupt the transmission of wild poliovirus and vaccine-derived poliovirus.

Objective:To explore the value of 5G robotic remote ultrasound in the diagnosis of plateau pulmonary edema(HAPE).Methods:A total of 27 patients who quickly entered Nagqu, Tibet at an altitude of 4 600 m-5 600 m from March to December 2021 and developed one of the clinical symptoms of HAPE were collected. All patients were examined by 5G remote robotic ultrasound and lung CT respectively. Kappa test was used to analyze the consistency of the two diagnostic results, and McNemar test was used to compare the difference in diagnostic results. The ROC curve was used to analyze the sensitivity and specificity of remote lung ultrasound scores in the diagnosis of HAPE.Results:Among the 27 patients, 16 showed thickening of pleural line, increasing of B line, lung consolidation, pleural effusion, etc. Meanwhile, 11 showed no abnormality. Additionally, 8 cases had diffuse pulmonary fluid in both lungs, and 8 cases had localized pulmonary fluid. ROC curve showed that the area under the curve of lung ultrasound score for the diagnosis of HAPE was 0.947 (95% CI=0.78-0.99, P<0.001). The sensitivity and specificity were 0.933 and 0.917, respectively. Lung CT diagnosis was positive in 15 cases. Lung CT showed thickening of lung texture, ground glass, small nodular shadow, fine reticulate shadow, etc. The diagnostic results of the two techniques were in good agreement (Kappa=0.924, P<0.001), and there was no significant difference between the two methods ( P>0.05). Conclusions:5G remote robotic ultrasound has high consistency with CT in the diagnosis of HAPE and is an alternative early diagnosis method for HAPE. It may have clinical application value in scattered medical resources and remote plateau areas.