The pharmacokinetics of Luteolin ( Lut ) solution in rabbits and rats was investigated. Lut in plasma, bile and urine as well as protocatechuic acid (which is a product of Lut) in plasma and urine were determined by fluorescence spectrophotometry. A curve with multiple peaks was fitted to the data of plasma concentration versus time, employing a nonlinear program developed with an IBM-PC computer. The results indicated that the data set obtained with 30 mg /kg doses by iv fitted well to a peculiar open 3 compartment model involved in the enterohepatic recirculation of the drug and the undulations of the drug concentration.The essential pharmacokinetics parameters (mean?SD) were as follows; The t1/2 values for the ?-phase and ?-phase were about 0.18?0.01 h and 1.66?0.24 h respectively. The apparent volume of distribution ( Vd) was about 1.43? 0.45 L?kg-1. The reabsorption rate constant ( Ka ) was 0.465? 0.08 h-1 The reabsorption percent ( F' ) was about 6.9? 2.1% . The elimination rate constant was 1.124?0.35 h-1. The clearance was 588?113 ?g?h-1?kg-1. This study indicated that Lut was rapidly removed from the blood by both kidney and liver where Lut was excreted by way of the bile into intestine from which it Was reabsorbed. The 12 h urine excretion of Lut in rabbits was in the order of 37.7% of total iv dose. In rats, the 6 h bile excretion of Lut was about 11.2% of that total iv dose. A comparison of the C-T curve of PCA with of Lut suggested that PCA was the one of the products of Lut.In rats, the 3H-Lut orally administered was widely distributed in the tissues, the concentrations being higher in liver and kidney.

Objective To explore the appropriate traction weight of traction method for the prevention of leakage of urine in elderly patients with indwelling urethral catheterization so as to provide a theoretical basis for clinical work.Methods A total of 120 indwelling urethral catheterization patients with leakage of urine in department of geriatrics of Jiangdu People's Hospital of Yangzhou (affiliated Hospital of School of Medicine, Yangzhou University) were selected by convenient sampling from January 2014 to August 2016. They were randomly divided into four groups and accepted traction method for the prevention of leakage of urine involving traction weight with 50, 100, 150, 200 g. The preventive effect and situation of comfort of patients were compared among four groups.Results The effective rate in prevention of leakage of urine of traction weight with 50, 100, 150, 200 g was 16.7%, 100%, 100%, 100% with a statistically significant difference among four groups with different traction weight (Hc=73.849,P<0.001). The results of intercomparsion showed that the effects of groups with traction weight of 100, 150, 200 g were statistically better than that of the group with traction weight of 50 g (χ2 = 42.857,P<0.001). There were statistically significant differences in degree of comfort among four groups with different traction weight (Hc=57.674,P<0.001). Compared with the group with traction weight of 150 g, the incidence of moderate or severe discomfort was 70% in the group with traction weight of 200 g with a significant difference (χ2=19.817,P<0.001). There was no statistically significant difference in the incidence of moderate or severe discomfort between the group with traction weight of 100 g and 150 g (χ2= 0.162,P=0.688).Conclusions The optimum traction weight for the prevention of leakage of urine in elderly patients with indwelling urethral catheterization is 100-150 g.