Objective To finally diagnose the outbreak of acute respiratory infection caused by Adenovirus 3 in students of the north of Jiangsu province by serologic study. Methods An enzyme-linked immunosorbent assay (ELISA) has been used to detect the adenovirus IgM in the serum of acute phase and the adenovirus IgG in the serum of recovery phase. A neutralization test has been used to detect the neutralization antibody in the serum of acute and recovery phase from cases and in the serum from the control. The SPSS11.0 has been applied to the statistical analysis of the data. Results The positive rate of IgM, IgG and recovery phase neutralization antibody of cases are 3.7%, 44.4% and 59.5% respectively, while those of control are 0%, 8.3% and 33.3% respectively, and the P values of Chi-Square are 0.510, 0.018 and 0.226 respectively. The neutralization test of paired serum of 6 in 9 cases showed that the antibody titers was obviously increased. The concordance between IgG detected by ELISA and neutralization antibody detected by neutralization test is 61.4% and the P value of Kappa is 0.070. Conclusion By the serologic study, we can finally diagnose that the outbreak of acute respiratory infection was caused by Adenovirus 3.

Objective: To study the pharmacokinetic progress of diclofenac sodium microemulsions in rabbits. Methods: Diclofenac sodium microemulsion and diclofenac sodium suspension were single orally given to rabbits. Diclofenac sodium concentrations in plasma were measured by HPLC method. Results: AUC 0 ∞ , c max1 and t max1 were 13.456 ?g?h?ml -1 , 2.852 ?g/ml and 1.438 h after po diclofenac sodium microemulsion and 10.584 ?g?h?ml -1 , 3.145 ?g/ml and 0.750 h after po diclofenac sodium suspension. Conclusion: The absorption of diclofenac sodium microemulsions in rabbits is slower and can keep a higher concentration in plasma for a long time compared with those of the suspension. [

Objective to investigate the current status of preoperative anxiety level and explore the influencing factors in elderly patients with pros-tatic hyperplasia. Methods A total of 124 patients with prostatic hyperplasia were selected for the study,and the State-trait Anxiety Inventory (StAI)was analyzed using the convenience sampling method. Results the score were 55.54±4.81 for SAI,34.60±2.09 for tAI,and 90.14±5.91 for StAI. the preoperative anxiety level of patients with prostatic hyperplasia varied in different demography(P < 0.05). According to multiple lin-ear stepwise regression equation,age,cultural level,place of residence,and the medical burden are the influence factors. Conclusion the preoper-ative anxiety level of elderly patients with prostatic hyperplasia is high,and more health education is needed. It is necessary to use different psycholog-ical persuasion according to their specific situation,so as to promote their physical and mental health,and to promote the effect of postoperative recov-ery.

Objective To evaluate the safety and immunogenicity of Shigella flexneri 2a conjugate vaccine.Methods A random and double blind study were carried out to compare the safety sero conversion rates and geometric mean titer(GMT)increase of Shigella flexneri 2a conjugate vaccine with phosphate-buffered saline used as control.Results The results showed that no severe systemic and local reaction rates occurred in trial group,which were not statistically significant compared with the control group.The seroconversion rate of both two weeks and twelve weeks after two doses(increased by 4 folds) were 86.27% and 79.74%;for GMT were 1∶361.83 and 1∶326.21 and increased averagely 1.08 and 0.98 times,which showed significant difference with those of the control group.Conclusion Shigella flexneri 2a conjugate vaccine was safe and its immunogenicity was good in group over 2 years old.Trialregistration National food drugs surveillance administrative bureau,"Medicine Clinical Experiment Written directive from a superior"2003L03808 number.

OBJECTIVE:To study the effects of Shuanghuanglian on pharmacokinetics of theophylline in healthy sub?jects.METHODS:The serum concentration of theophylline was determined by HPLC and analyzed by3p97pharmacokinetic program.RESULTS:The main pharmacokinetic parameters of single theophylline and that in combined use with Shuanghuan?glian were as follows:T max were(1.66?0.56)and(1.59?0.78)h,C max were(6.23?1.31)and(6.10?0.94)?g/ml,T 1/2 were(5.76?1.11)and(6.09?1.63)h,CL were(47.72?5.12)and(50.98?10.85)ml/(kg?h),Vd were(369.18?40.15)and(430.37?48.33)ml/kg,AUC 0～∞ were(84.56?14.43)and(89.27?26.35)?g/(h?ml),respectively.CONCLUSION:The Vd of theophylline were increased(P

OBJECTIVE:To improve the pharmacotherapeutic efficacy for kidney transplant patients and decrease the adverse drug reactions METHODS:Clinical pharmacists intensified the monitoring of blood drug concentrations,adjusted the doses and kinds of drug used,established the pharmaceutical records for the recipients and guided the use of drugs RESULTS & CONCLUSION:The incidence of adverse drug reactions could be effectively decreased in kidney transplant patients by the way of pharmaceutical care

OBJECTIVE:To evaluation of the efficacy and safety of azithromycin in the treatment of acute infections METHODS:A randomized controlled multicenter clinical study was used to compare the clinical efficacy of azithromycin(200mg q d for 3～5 days) with erythromycin(500mg b i d for 5 days) RESULTS:The cure rate,effective rate,and bacterial clearance rate were 76 7%,95 0%,and 94 3% respectively for azithromycin group and were 48 3%,76 7%,and 77 4% respectively for erythromycin group with a side-effect incidence of 10 0% for azithromycin group and 39 3% for erythromycin group CONCLUSION:Azithromycin is superior to erythromycin in clinical efficacy,safety and side-effect incidence

OBJECTIVE:To prepare ofloxacin hollow suppository(OHS) and to observe its in vitro dissolubility.METHODS:OHS was prepared with PEG 6000,PEG 400 and Carbopol-940 as wase material and wax as retardant.The content of ofloxacin in OHS was determined with the first order derivative UV-spectrophotometry.RESULTS:The wax,as a retardant,could retard the release of drug.The in vitro dissolution of OHS revealed the first order dissolution pattern:K0～1=27.81/h,indicating a speedy effect,K2～6=5.94/h,indicating sustained-release effect.CONCLUSION:This preparation is feasible in technology and controllabe in quality.The preparation of OHS extends the kinds of dose-form of ofloxacin

OBJECTIVE:To provide guidance for pharmacists to adopt a right method in participating in clinical drug treatment work.METHODS:In participating in clinical drug treatment work,pharmacists identified physicians’ drug treatment schemes by sticking to Guiding Principle of Clinical Use of Antibacterials and made remarks on the evaluation results.RES-ULTS & CONCLUSION:Through participating in clinical medical quality ward round,pharmacists can bring their professional advantages into full play,timely find out problems existed in the clinic drug treatment,guide clinical rational drug use,and play a key role in promoting drug treatment.

Objective: To study the pharmacokinetics of diclofenac sodium microemulsions in human. Methods: According to the crossover design, each volunteer was orally given diclofenac sodium microemulsion and diclofenac sodium tablet. The serum concentrations were determined by RP-HPLC with UV-detector. The concentration-time data were analyzed using 3P87 Pharmacokinetic Program and the pharmacokinetics parameters were compared by paired t-test. Results: It was found that diclofenac sodium in serum was linear within the range of 50-8 000 μg/L. The minimum detection concentration was 30 μg/L. The mean rate of recovery was (100.55±1.56)%. After a single oral dose, AUC0～∞ were 5.563，7.891 μg*h/ml, MRT 5.489， 5.387 h for dispersible diclofenac sodium microemulsion and tablet respectively. Conclusion: Absorption progress of diclofenac sodium microemulsion in human may be special.