Objective To investigate the safety and clinical response of dendritic cells (DC)vaccine loaded with HLA-A2-restricted peptides MAGE-3、 Tyr、 MART-1 and GP-100 against malignant melanoma. Methods Twenty three HLA-A2 positive patients with malignant melanoma were enrolled.Peripheral blood mononuclear cells were separated into adherent cells for induction of DC loaded with four peptides. DC vaccine was administered subcutaneously once a week in inguinal region. The immunological responses and clinical responses were evaluated. Results Dendritic cell vaccination were well tolerated in all patients and there was no toxicity observed. Serum levels of IL-2, IL-12 and IFN-γincreased significantly after first vaccination and fourth vaccination. The positive rate of DTH test was 50% (5/10), and 2 fold increase of CD8+ IFN-γ+ cells were observed in 6 of 10 patients. Stable disease was observed in 11 of 23 patients, one patient had a complete metastasis regression, four patients had 50% regression of metastasis,four patients had a minor response, and disease progressed in three patients. Conclusion DC vaccines loaded with peptides MAGE-3、 Tyr、 MART-1 and GP-100 can elicit non-specific and specific immune responses, leading disease control. DC vaccine is considered one of safe and effective treatments for malignant melanoma.

Objective: To explore the influence of sports rehabilitation training on mental health, sports consciousness and sports behavior of disabled college students. Methods: A total of 108 students with mild to moderate physical disability from 6 universities in Tianjin City were selected as the research objects. They were divided into two groups ( n =54) by random number table method. One group was used as a blank control without any intervention (control group). The other group was used for sports rehabilitation training through sports prescription (experimental group). The changes of physical condition, mental health, exercise consciousness and exercise behavior of the two groups before and after intervention were compared. Results: The positive rates of anxiety, depression, interpersonal sensitivity (25.93%, 12.96%, 22.22%) in the experimental group were significantly lower than those in the control group( χ 2=6.64, 4.48, 16.80, P <0.05). Compared with that before intervention, the proportion of college students in the experimental group whose goal was to exercise willpower, assist in treating diseases, satisfy interests and promote health (29.63%, 24.07%, 35.19%) after intervention was significantly increased, and the proportion of college students whose goal was to deal with physical examination (14.81%) was significantly reduced ( χ 2=4.48, 5.65, 5.98, 32.04, P <0.05). Compared with that before intervention, the frequency and time of exercise per week in the experimental group increased significantly after intervention, and the proportion of the main forms of exercise in the experimental group increased, the proportion of low intensity exercise decreased, the proportion of high intensity and medium intensity exercise increased, the proportion of irregular exercise decreased, the proportion of regular exercise increased, the proportion of those who felt good after exercise increased. The differences of each group were statistically significant ( χ 2=17.84, 14.72, 16.34,15.15, 16.12, 9.44, P <0.05). Conclusion The exercise rehabilitation training for the disabled college students is helpful to strengthen their sports consciousness and improve their sports behavior, so as to effectively improve their physical health, reduce their negative emotions such as anxiety, self blame, allergy and depression, and promote their mental health.