Climacteric ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hyperthyroidism ; drug therapy ; Methimazole ; therapeutic use ; Middle Aged ; Phytotherapy

The family of bone morphogenetic proteins (BMPs) belongs to the superfamily of transforming growth factor-β (TGF-β). It has various functions in the body. In recent years the BMPs family(especially the BMP7) has been discovered to play an important role on the genesis and development of cancer. In this article the characteristic, the mechanism and the function of BMP7 in the genesis and development of cancer are summarized.

China ; Environmental Exposure ; prevention & control ; standards ; Hot Temperature ; Humans ; Occupational Exposure ; standards ; Seasons ; Temperature ; Workplace ; legislation & jurisprudence ; standards

Objective: To discuss the management principles and skills for treatment of intractable ureterostenosis under ureteroscope. Methods: Our management experience on 19 patients with intractable ureteral stenosis was retrospectively analyzed. The 19 cases included urological TB-caused multiple ureteral stenosis, oncothlipsis to ureters from intestinal tract or gynecology, restenosis 3 months to 12 years after pelviureteric junction plasty, operative site stenosis after ureterolithotomy, double ureter back flow accompanied by stenosis, ureter imperforation after renal parenchyma lithotomy without placing double "J", ureter imperforation 3 months after extracorporeal shock-wave lithotripsy due to ureterolith, tubal bladder stoma stenosis after renal transplantation, restenosis after tubal bladder stoma due to distal ureterostenosis, and so on. All the patients were treated under ureteroscope. The management methods included: the Wolf 8/9. 8 CH12° and Wolf 6/7. 6 CH5° ureteroscope was used as a dilator to dilate the stenoses; balloon expanding under ureteroscope was used to dilate the stenoses; the ureter pliers was used to expand the stenoses to different directions; the cold knife was used to open the stenoses; if the diameter of stenoses were smaller than the that of the ureteroscopes, F4. 5 or F3 double "J" tubes were inserted guided by a wire under ureteroscope; and 2 or 3 weeks later, a larger tube or two tubes were introduced into the stenoses already dilated partly by the former tube. Results: Ureteroscopic method failed in treating 2 patients in our group and succeeded in treating all the other patients. The outcomes of patient were fine during 2 months to 3 years' follow-up. Conclusion: It is difficult to treat patients with intractable ureterostenoses. With good experience in manipulation of ureteroscope, the flexible application of several techniques according to the different conditions of different patients can guarantee successful treatment in most patients.

Objective: A rapid and sensitive method using liquid chromatography-tandem mass spectroscopy (LC-MS/MS) was developed and validated for the simultaneous quantitative determination of salvianolic acid B (Sal B) and its main active metabolite danshensu(DSS) in rat plasma. Methods: The analytes were extracted by liquid-liquid extraction with ethyl acetate after IS (IS, chloramphenicol) spiked. The separation was performed on a Symmetry C18 column with methanol-acetonitrile-0.5% formic acid (55:5:40) as mobile phase at a flowrate of 0.4 mL/min. The triple quadrupole LC-MS system was operated under selected reaction monitoring (SRM) mode using the electrospray ionization technique in negative mode. Quantification was performed using SRM of the transitions m/z 717→519 for Sal B, 197→135 for DSS, and 321→152 for the IS, respectively. Results: The nominal retention times for Sal B, DSS , and IS were 3.12, 2.60, and 3.98 min, respectively. The standard calibration curve for spiked rat plasma containing Sal B was linear over the range 10-5 000 ng/mL with a correlation coefficient (r >0.995). And DSS was linear over the range 5-5000 ng/mL with a correlation coefficient (r>0.995). The lower limits of quantification (LLOQ) of Sal B and DSS of the method were 10 and 5 ng/mL, respectively. The intra- and inter-day accuracy and precision of the assay were less than 12.6%. After Sal B was ig administered to rats, absorption of Sal B was rapidly metabolized to DSS. Pharmacokinetic parameters of Sal B and DSS after ig administration Sal B to Wistar rats were: Cmax (1.21±0.31) and (0.27±0.05) μg/mL, tmax (0.50±0.00) and (0.56±0.18) h, t1/2 (1.20±0.11) and (1.57±0.16) h, AUC0-1 (1.31±0.30) and (0.39±0.05) μg·mL-1·h. Conclusion: This method has been applied successfully to a pharmacokinetic study of Sal B and its metabolite DSS involving the ig administration of Sal B to rats.

Objective To investigate the expressions and clinical significance of Id1 in diffuse large B‐cell Lymphomas (DL‐BCL) tissue and Id1 gene in bone warrow cell .Methods Forty cases of DLBCL(observation group) and 25 cases of reactive lymph‐oid hyperplasia (control group) were included in this study which were admitted by our hospital from October 2011 to October 2014 .The expression of Id1 proteins in DLBCL and reactive lymphoid hyperplasia were detected by imunohistochemical technique . The expression of Id1 genes in all patients′marrow cells was detected by reverse‐transcriptase polymerase chain reaction .The data were collected and analyzed by designed person .Results In 40 DLBCLs ,the positive rate of Id1 were 75 .00% (30/40) ,which was higher than in RHs 32 .00% (8/25) ,with statistic difference(P= 0 .001) .Id1 protein was not correlated with sex and age(P>0 .05) ,but was correlated with clinical stage ,LDH level and extranodal infiltration(P<0 .05) .The expression of Id1 genes in mar‐row cells in DLBCL was higher than in RH (Id1mRNA level 2 .80 ± 0 .87 vs .1 .37 ± 0 .51 ,P<0 .05) ,and also correlated with clini‐cal stage ,LDH level and extranodal infiltration(P<0 .05) .Conclusion The expression of Id1 proteins and genes are much higher in DLBCL tissue and marrow and probably related to the prognosis of DLBCL .This discovery would contribute to predicting prognosis of DLBCL ,w hich also could be a therapeutic target of DLBCL in the future .

Objective　The clinical manifestations and mechanism of pathological synkinesia were studied. Methods　Recurrent pathological synkinesia was measured by clinical and experimental nictitation reflex. The changes of nerve-muscle junctions were observed via scanning electron microscope. Results　All the 32 cases of pathological synkinesia were denervation of degree Ⅲ and severe facial palsy. Reaction undulatory forms of musculus orbicularis oculi and musculus orbicularis oris recorded simultaneously at the stimulated side in trigemial-facial nerve reflex test accounted for 81%(28/32) patients and 89% guinea pigs. Conclusion　Pathological synkinesia was the marker of severe facial palsy and was related with over-error in regeneration of nerve.

Objective Based on the investigation of household drinking water in Lanzhou, the reason and difference of selecting drinking water by different households of educational and earning level were analyzed. Methods 1 000 households in Lanzhou city were chosen to fill the questionnaires, and the statistical analysis were performed. Results The tap water was chosen by 65.3% of Lanzhou households. 29.8% Lanzhou households drank barreled drinking water. The "convenience" and "price" were major concerns when Lanzhou residents chose drinking water. The acceptable price of barreled drinking water was 7.2 yuan(RMB) per barrel. The households of high income and well educated were the principal households using depth treatment water as drinking water. Residents in Lanzhou city had little concerns about quality of tap water. Conclusion The tape water was chosen by a majority of Lanzhou households as drinking water. The price was the major reason for Lanzhou residents to choose drinking water. In order to keep healthy,the residents should choose safe and healthy drinking water,and they had better clean the trough.

Objective To know the effect of hours of use of the drinking machine on drinking water quality. Methods The hours of use of the drinking machine were 1week, 2, 5, 10, 12, 36 and 60 months, the water samples collected at different hours of use was determined by routine test methods of water quality. Results The total bacteria count and nitrite in drinking water exceeded the standard limits and the two indexes increased as the hours of use of the drinking machine lasted. At the first-using of drinking-machine, the total bacteria count and coliform count exceeded the standard limits seriously. Conclusion In order to ensure the drinking water safety,water should be boiled before drinking and the drinking-water-producing factories should improve the production and management. Cleaning and disinfection of the inner components of drinking water machine should be paid more attetion to.

Objective:To explore the effects of Ganoderma Lucidum spore on the immunity of the immunosuppression mice with Glucocorticiods(GC).Methods:Different dosages of Ganoderma Lucidum spore were intragastriced to the immunosuppression mice with Glucocorticiods, and the weight of the immune organs and the phagocytic function of the macrophages and neutrophile,the expression in of CD3+,CD4+,CD8+,CD49b+ and CD19+ in the blood cell were measured;And the total complement activity, the content of the cytokine and the valence of hemolysin in the mice serum were detected.Results:The immune function of the GC immunosuppression mice were remarkable effected with Ganoderma Lucidum spore, it could significantly increase the index of the thymus and spleen, improve the valence of hemolysin and the phagocytic function of the macrophages and neutrophile of the mice.The total complement activity, the content of the cytokine in serum,and the quantity of T,B and NK cell in the mice blood were influenced by Ganoderma Lucidum spore.Conclusion:The results show that Ganoderma Lucidum spore remarkable effect on the immunity of the immunosuppression mice with GC, and it can promote accommodation the immune function of mouse.