Impaired recognition of foreign nucleic acid initiates abnormal mucosal immune responses in intestine.Very little is known on how microbial DNA activates intracellular DNA sensors and controls the function of antigen-presenting cells to shape mucosal immune responses in inflammatory bowel disease (IBD).In current review,we will introduce mucosal dendritic cell population,describe newly identified cGAS-cGAMP-STING complex,and identify certain IBD susceptibility genes that associate with impaired microbial DNA recognition in human.

During medical activity,there are theory and realism spring of that the right of emergency treatment is in conflict with the right of informed consent.Simply investigating responsibilities is of no avail to solve the conflicts.To Interpret the real reasons of the conflict through legal and ethical aspects.To avoid and reduce conflict,the effective measures are that perfecting the rules of relevant law,elucidating the rights and obligations of hospital and medical staff;regulating the contents of informed consent form and strengthening medical ethics education.

OBJECTIVE: To investigate the statistical method of tri-allelic patterns of STR loci. METHODS: The DNAs of venous blood and blood stain samples from 8,846 unrelated individuals were extracted using magnetic bead method, and STR genotypes were determined by multiplex fluorescent amplification and capillary electrophoresis and analyzed using GeneMapper ID v3.2 software. The genotype frequency and allele frequency of trizonal were determined by direct counting and formula, respectively, in order to deduce the formula of trizonal in paternity test and individual recognition. RESULTS: Of the 8,846 individuals, four tri-alleles and three tri-genotypes were detected. The multiplying of allele frequency and actual rate showed significantly statistical difference. The formula of trizonal in paternity test and individual recognition was successfully deduced. CONCLUSION The frequency of the two alleles inherited as a whole in the population could be calculated by multiplying the frequencies of each allele in the tri-allelic patterns.
Alleles ; Gene Frequency ; Genetic Loci ; Genetics, Population ; Genotype ; Humans ; Microsatellite Repeats ; Polymorphism, Genetic

Objective To probe the neuroprotective effect of memantine in hippocampal CA1 region of rats with vascular dementia(VD). Methods 36 male Wistar rats were randomly divided into sham-operated group,VD group and memantine group,and each group have 12 rats. Rats of VD were treated with a permanent bilateral common carotid arteries occlusion(2-VO). Then memantine was given by intragastric administration.The behavioral abnormalities were investigated by Y-type maze test.The changes of cell morphology and the expressions of neuronal nitric oxide synthase(nNOS) and cysteine proteinase-3 (Caspase-3) of neuron in hippocampal CA1 region were detected by HE stain and immunohistochemistry respectively.The percentage of neuronal apoptosis by TUNEL. Results Compared with VD group and sham-operated group,the performance records of learning and memory(learning records:63.16±1.75,79.25±2.01,50.83±1.80,F =707.91,P <0.01;memory records:53.17±1.85,69.17±1.70,40.67±1.83,F =762.43,P <0.01),the expressions of nNOS (average gray scale:140.23±2.30,175.87±3.34,105.63±3.52,F =1539.67,P <0.01)and Caspase-3 (average gray scale:132.78±5.36,162.78±5.25,99.54±5.06,F =439.37,P <0.01)and the percentage of neuronal apoptosis (33.75±2.70,56.17±3.56,6.33±3.65,F =672.91,P <0.01)of memantine group were in the middle. Conclusion Memantine can improve spatial memory and attenuate neuronal apoptosis by down-regulation excessive expression of nNOS and Caspase-3 in rats with vascular dementia.

Transient ischemic attack (TIA) is the most important early warning signal of cerebral infarction, and it is also the critical period for the prevention of cerebral infarction. If we effectively control the risk factors of TIA, the onset and the progress of TIA can be prevented and controlled, This article reviews the correlation between the risk factors of TIA and prognosis.

Objective To understand the operation room nurses' job stress and mental health status and discuss the countermeasures to ease pressure. Methods 82 patients were selected from five hospitals from April to August 2010. We used symptom checklist, self-rating anxiety scale, self-rating depression scale and nurse' job stress scale to carry out the questionnaire survey. Results The pressure of operation room nurses were related with their mental health status. Conclusions Some problems existed in mental health and work stress in operation room nurses, which should be highly valued by operation room nursing managers.

Excitatory amino acid toxicity is one of the mechanisms of vascular dementia. Gluta-mate is the major excitatory neurotransmitter in brain. Memantine, a moderate affinity, uncom-petitive and voltage-dependent N-methyl-D-aspartate (NMDA) receptor blocker, can block NMDA receptor-induced intracellular calcium overload and prevent excitotoxicity. It can be used in the treatment of vascular dementia.

Objective To establish a thin layer chromatography scanning method for the determination of cinnamaldehyde content in Qianzheng Plaster.Method Cinnamaldehyde was extracted from Qianzheng Plaster by distillation method.Silica G was used as the thin-layer plate;petroleum ether (60～90℃) and acetic ethyl acetate (17 ∶3)served as the mobile phase and the scanning wavelength was 269 nm.Results A good linearity was obtained in the range of 0.81?g～4.05 ?g (r=0.9993).The mean recovery rate was 95.23 %and RSD=1.39 %.Conclusion This method is accurate and sensitive with good reproducibility.

Thirty-six subclinical hypothyroidism patients with different pathogenic background were treated by levothyroxine. After treatment the levels of serum total cholesterol, low density lipoprotein and triglyceride were significantly lower than those before treatment (P