Objective:To study the the mental health and relevant factors of patients with hepatitis B. Methods: 58 cases of patients with hepatitis B were assessed with the Social Support Scale, Symptom Checklist-90 (SCL-90), Trait Coping Questionnaire and compared with healthy person. Results: All patients had obvious psychosomatic symptoms. The mental health level was negatively correlated with postive coping style and social support. Conclusion: Several psychosocial factors played an important role in the onset and trealment of Hepatitis B disease.

Objective To compare the neurotoxic effects of intratheeal ropivacaine and bupivacaine on the spinal cord in rats.Methods Female SD rats weighing 240-330 g were used in this study.Catheters(PE 10 tubing)were inserted through an incision in the cisternal membrane and advanced caudad until the tip of the catheter reached the lumbar spinal level according to the technique described by Yaksh and Rudy.Fifty-four successfully catheterized rats were randomly assigned tO one of 3 groups(n=18 each):group Ⅰ received normal saline 30μlIT(NS);groupⅡ received 2%bupivacaine 20μl IT(BU)and group Ⅲ 2.7%ropivacaine 20μl IT (RO).Degree of motor block was assessed and scored(0=no block,2=inability to flex the hind limb)before (T1,baseline)and at 10,20,30,60 and 120 min(T2-6)after IT injection.On the 4th day after IT injection specimens were obtained from lumbar spinal cord for microscopic examination.Results The motor block score was significantly higher in group BU and RO than in group NS(P＜0.05),and the tissue damage was severe in group BU and RO.The motor block scores were significantly higher at T2 than at T3-6 in beth group BU and RO(P＜0.05).There Was no significant difference in motor block score at T2 between groups BU and RO but the motor block scores were significantly lower at T3-5 in group RO than in group BU(P＜0.05).The tissue damage was Severer in group RO than in group BU(P＜0.05).Conclusion Intrathecal 2.7% ropivacaine is more toxic to the spinal cord than intrathecal 2%bupivacaine in rats.

With the increasing perfection and improvement of microsurgery technique in modern ophthalmology, especially the popularization of micro-incision cataract extraction and the rise of femtosecond-laser-assisted cataract extraction, the cataract extraction and intraocular lens implantation is gradually transformed from vision rehabilitation surgery into refractive surgery and people also pursue better postoperative visual experiences. However, the preoperative and postoperative corneal astigmatism is always the main factor affecting the postoperative visual effect. ln recent years, the development of design and materials of the intraocular lens ( lOL) provided necessary basic conditions for clinical application of the Toric lOL. The Toric lOL is primarily characterized by effective correction of cataract patients′postoperative corneal astigmatism, which results in the enhancement of patients′postoperative visual function. ln this paper, the design, classification and clinical application of Toric lOL were analyzed, and current problems and prospect were discussed.

Objective To study changes in expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the kidney and spleen of dead rats and its application in deducing the postmortem intervals (PMI), so as to look for a new method for PMI deduction. Method 28 rats were killed by cervical dislocation, placed in a chamber with temperature-controlling system at 20℃ for 0 to 48h postmortem, and autopsied at 0, 8, 16, 24, 32, 40 and 48h respectively (n=4 for each time point). The expression of GAPDH mRNA in kidney and spleen was assessed with fluorescence semi-quantitative RT-PCR. Results The amplified products of GAPDH mRNA could be detected in kidney within 40h and in spleen within 32h postmortem respectively, and they showed a decreasing tendency as its fluorescence intensity declined gradually and lost at last. Conclusion Detection of GAPDH mRNA expression in kidney and spleen of rats at different periods after death by fluorescence semi-quantitative RT-PCR seems to be of some value for estimation of PMI.

30ml) undergoing minimally invasive evacuation of intracranial hematoma in our department were analyzed retrospectively.The chants of IL-1,IL-6 and IL-8 in hematoma fluid were observed continuously.The content of IL-1,IL-6 and IL-8 was determined by radioimmunassay.Results IL-1,IL-6 and IL-8 were observed at 6～12h after hypertensive cerebral hemorrhage,and showed different changes in acute stage.Conclusion Inflammatory cytokines are involved in the pathological process of cerebral hemorrhage.

Objective:To investigate the effects of enteral nutrition assisted therapy in preventing postoperative anastomotic fistula in patients with esophageal cancer and its influence on immunity,healing process and nutritional recovery.Methods: The clinical data of 90 cases of patients with esophageal cancer who underwent surgical treatment were retrospectively analyzed.According to the mode of postoperative nutritional support,the patients were divided into group A(n=34),B(n=30)and C(n=26)three groups.Group A was treated by immune-enhancing enteral nutrition(Supportan)assisted therapy.Group B was treated with routine enternal nutrition(Nutrison Fibre)assisted therapy.Both of the two groups were treated with 25%,50% and 100% of the full dose on the 1st,2nd and 3rd-7th day after surgery.Later,the dose was reduced day by day till normal diet.Group C was treated by parenteral nutrition assisted therapy.Since the 1st day after surgery,patients in group C were intravenously injected with glucose,vita min and a mino acid mixed liquid calculated by 125.52 kJ/kg.8-10 d later,patients gradually transited to the normal diet.The incidence rates of anastomotic fistula,pulmonary infection and incision infection,wound healing time,total hospitalization time and initial exhaust time were observed and compared between the three groups.The changes of immune index [T lymphocytes and its subsets(CD3+,CD4+,CD8+)],inflammatory factors[C reactive protein(CRP),IL-6]and nutrition indexes[serum total protein(TP),albumin(ALB)]before surgery,on the 1st and 8th day after surgery were recorded.Results: ①There were significant differences in the incidence rates of postoperative anastomotic fistula and pulmonary infection between the three groups(P<0.05),and the incidence rates in group C were significantly higher than those in the other two groups(P<0.05).②There were significant differences in wound healing time,total hospitalization time and initial exhaust time between the three groups(P<0.05)and that of group C group was longer than that of the other two groups(P<0.05).③On the 1st day after surgery,the levels of immune indexes such as CD3+,CD4+,CD4+/CD8+ and nutrition indexes such as TP and ALB in the three groups were significantly lower than those before surgery.CD8+ levels and levels of inflammatory indexes such as CRP and IL-6 were significantly higher than those before surgery(P<0.05).However,there was no significant difference between the groups(P>0.05).On the 8th day after surgery,the nutrition indexes in the three groups were still significantly lower than those before surgery.However,those in group A and B were significantly higher than those in group C(P<0.05);the inflammatory factors were still significantly higher than those before surgery(P<0.05)but those in group A and B were significantly lower than those in group C(P<0.05).Among the three groups,there was no statistical significance in comparing the immune indexes with those in group A(P>0.05).The levels of CD3+,CD4+,CD4+/CD8+ in the other two groups were lower than those before surgery while CD8+ level was higher than that before surgery(P<0.05).Conclusion: To apply enteral nutrition assisted therapy in patients with esophageal cancer after surgery can effectively improve the prognosis.It has positive effects on shortening the healing process,improving the immune function and improving the nutritional status.

BACKGROUND:Anterior cruciate ligament (ACL) has been reported to hold a self-recovery potential, which may be related to certain cytokines and biological factors, such as stem cells or progenitor cells.OBJECTIVE: To review the literatures about the relationship between mesenchymal stem cells and ACL injuries, and understand the potential of stem cells or progenitor cells differentiating into ACL, thus providing a basis for the clinical use of mesenchymal stem cells in the treatment of ACL injury. METHODS:A computer-based online research of PubMed databases was performed to collect articles including reviews, clinical trials and basic studies using the English keywords of ACL regeneration, stem/progenitor cells, mesenchymal stem cells. Forty-five eligible articles were included finally. RESULTS AND CONCLUSION:In recent years, there are a lot of studies addressing the treatment of ACL injuries and muscle/bone regeneration, but the use of stem cells is still far from the clinical requirements. The source of stem cells suitable for the ACL regeneration and their most suitable injection points are under discussion. Although the mesenchymal stem cells have been reported to successfully repair ACL injury in animal models, either trophic factors of stem cells or these cells themselves improving the regeneration remains unclear.

AIM: To elucidate the effects of platelet-activating factor (PAF) on eosinophil activation and the action of dexamethasone or theophylline during this process. METHODS: Eosinophils (EOS) from the peripheral blood of normal subjects were isolated. The hypodense eosinopil (HE) and normodense eosinophil (NE) were studied with electron microscopy. The effects of PAF on eosinophil activation and the action of dexamethasone or theophylline during the above process were measured. RESULTS: Hypodense eosinophil had significantly smaller individual granules than normodense eosinophil had. PAF induced eosinophil peroxidase release, and generated. Eosinophils incubated with 10~(-8) mmol/L PAF and 10~(-5) mmol/L dexamethasone released (101.17?10.32) mg/L eosinophil peroxidase (P

Objective To study difference of coronary artery lesion between Han nationality and Uygur nationality patients with coronary artery disease .Methods 2008 patients with coronary artery disease were analyzed retrospectively in our hospital .The cor-onary artery lesion was evaluated by the number ,location ,type of coronary lesion and Gensini scores .Results The incidences of three-vessel disease in Uygur patients (40 .6% ) were higher than that of Han patients (35 .3% ) .Along with the age increasing , Gensini scores increased gradually .Gensini scores of uygur patients were significantly higher than those of Han patients in same age groups .Compared with the Han patients ,mean age in the Uygur patients was lower(P<0 .05) .Conclusion Severity of coronary artery disease increased with age ,single-vessel lesion gradually reduced and three-vessel lesion gradually increased .The coronary artery lesions of Uygur patients were more severe than those of Han patients in same age :more three-branch lesion .Compared with Han patients ,morbidity age of similarly degree coronary atherosclerosis in the Uygur patients maybe more early .

[Objective] To investigate the effects of Qianggu capsule plus alendronate on postmenopausal osteoporosis.[Methods]By random number generating method,120 patients were randomly divided into a treatment group with Qianggu capsule plus alendronate,control group A only with Qianggu capsule,and control group B only with alendronate.The pain of the patient before treatment,3 m and 6 m after treatment was assessed.At the same time,the bone mineral density (BMD) before and 6 months after treatment was recorded.[Results] There was significant difference before and after 3 m in the score of pain in three groups,but the difference in treatment group was higher than other two control groups.After 6 m,all the scores of pain in 3 groups had changed significantly compared to the ones before treatment,but the scores of pain in treatment group were lower than 2 control groups.The BMD of both vertebral and ward in treatment group after 6 months treatment was higher than before.[Conclusions]Qianggu capsule plus alendronate has definite therapeutic effects on postmenopausal osteoporosis.