Objective To investigate the effects of electroacupuncture at meridian point on the mRNA expressions of corticotropin-releasing factor (CRF) and corticosteroid (CORT) in the hippocampus after cerebral ischemia reperfusion in rats.MethodsA total of 150 SD rats were randomly divided into 5 groups by random number table method: normal control group, sham operation group, model group, meridian point electroacupuncture group and non-meridian point electroacupuncture group, with 30 rats in each group. A model of focal cerebral ischemic reperfusion was induced using the modified intraluminal thread method. In the meridian point electroacupuncture group, at 6 h after cerebral ischemia reperfusion, acupuncture “LI11” on both sides, bilateral “ST36”, “GV20”, ”GV16” daily for 7 d. The neurological deficits were evaluated 1 d, 3 d, and 7 d after cerebral ischemia reperfusion, and then all rats were sacrificed and the hippocampi were harvested. The mRNA expressions of CRF and CORT in the hippocampus were determined by quantitative real-time PCR.ResultsThe mRNA expressions of CRF (1.122 ± 0.249, 1.190 ± 0.666, 0.454 ± 0.612 in the model group; 0.021 ± 0.049, 0.021 ± 0.027, 0.035 ± 0.005 in the sham operation group) and CORT (0.917 ± 0.113, 1.024 ± 0.290, 0.709 ± 0.055 in the model group; 0.016 ± 0.013, 0.016 ± 0.006, 0.043 ± 0.006 in the sham operation group) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly increased in the model group compared with the sham operation group (all P<0.01). The mRNA expressions of CRF (0.424 ± 0.104, 0.339 ± 0.476, 0.095 ± 0.021) and CORT (0.377 ± 0.073, 0.138 ± 0.025, 0.158 ± 0.010) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (allP<0.01). The neurological deficits scale 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (1.83 ± 0.75, 1.50 ± 0.55 and 1.17±0.41 in the meridian point electroacupuncture group; 2.50 ± 0.84, 2.33 ± 0.52 and 1.67 ± 0.52 in the model graoup; allP<0.01). Conclusion Electricacupuncture at meridian point can reduce the mRNA expressions of CRF and CORT in the hippocampus, and improve neurological deficits after cerebral ischemia reperfusion in rats.

BACKGROUND:White blood cells involved in the inflammatory response play an important role in deep vein thrombosis.E-selectin mediates the initial process of white blood cell and vascular endothelial cell attachment during inflammation.OBJECTIVE:To investigate the relationship between deep venous thrombosis(DVT) and plasma E-selectin.DESIGN,TIME AND SETTING:Completely random design and linear correlation analysis were performed at Department of Vascular Surgery,First Affiliated Hospital of Chongqing Medical University and Clinical Diagnostic Laboratory of Chongqing Medical University from September to December 2007.PARTICIPANTS:Twenty-seven cases of deep vein thrombosis,16 males and 11 females with an average age of(57 ? 15) years,were selected,who attacked within 3 days or with aggregated condition.METHODS:Blood samples were obtained at the admission of the patients.After 72 hours,a second specimen was obtained,defined by thrombolysis and anticoagulant therapy.Determination of plasma E-selectin by ELISA and the blood coagulation abilities and platelet count of the 1st blood samples were performed,including part-time activation thromboplastin,prothrombin time,and plasma fibrinogen.MAIN OUTCOME MEASURES:E-selectin levels before and after treatment,blood coagulation abilities before treatment,platelet determination and E-selectin related analysis.RESULTS:No acute pulmonary embolism occurred in 27 cases of deep vein thrombosis during the treatment.One case underwent routine Forgart of thrombectomy catheter,and of the other 26 cases,15 were significantly improved,and 11 were not improved after 72 hours.Plasma E-selectin levels significantly decreased after thrombolysis,anticoagulant therapy in deep vein thrombosis patients with clinical remission(P = 0.001),but the plasma E-selectin levels were increased in those with no remission(P = 0.003).E-selectin levels and platelet count of deep vein thrombosis in patients before treatment showed no correlation(r =-0.113,P = 0.576),and were not related to fibrinogen content(r =-0.050,P = 0.802),or part-time activation thromboplastin(r=-0.046,P=0.822),and prothrombin time(r=-0.080,P=0.690).CONCLUSION:Plasma E-selectin levels are positively related to deep vein thrombosis severity.

Objective To study the effect of environmental estrogen, bisphenol A (BPA), on proliferation of human uterine leiomyoma cells in culture. Methods The primary culture and subculture of the human uterine leiomyoma cells which were identified at each passage by means immunocytochemical staining with a monoclonal anti-?-smooth muscle actin antibody, were performed. The cells (passage 3-5) were incubated with BPA (including three dosimetric systems, 1?10-7 mol/L, 50 ?10-7 mol/L and 100?10-7 mol/L) for 24, 48 and 72 h, and the control cells received only the vehicle (ethanol, 0.1 %, V/V). The proliferation of cultured human uterine leiomyoma cells was analyzed by MTT assay and flow cytometry. Results The limited human uterine leiomyoma cell lines were successfully established. After 24 h, 48 h and 72 h of treatment in 100?10-7 mol/L and 48 h, 72 h, at 50?10-7 mol/L , BPA could enhance the cells proliferation. After 72 h of treatment in 50?10-7 mol/L and 100?10-7 mol/L, BPA promoted cells into S cell cycle phase and elevated mitotic index (P

Objective To investigate the effects of low tidal volume ventilation on pulmonary and cardiac function before and after mitral valve replacement. Methods Thirty patients with mitral valve diseases were randomly divided into traditional tidal volume ventilation (groupⅠ), low tidal volume ventilation with conventional respiratory rate (group Ⅱ), and low tidal volume ventilation with high respiratory rate (group Ⅲ). Mean arterial pressure (MAP), cardiac output(CO), cardiac index(CI), mean pulmonary arterial pressure (MPAP), pulmonary capillary wedge pressure (PCWP), central venous pressure (CVP), systemic vascular resistance index (SVRI), and pulmonary vascular resistance index (PVRI) were monitored. Arterial oxygen tension (PaO_2), carbon dioxide tension (PaCO_2), oxygen saturation index(PaO_2/FiO_2), alveolar-arterial PO_2 gradient (PA-aO_2), and Q_S/Q_T were measured. Results Before CPB, CO in group Ⅰ was significantly lower than that in group Ⅲ (P

Objective　 To investigate the changing spectrum and antibiotic resistance of the pathogenic bacteria isolated from general surgical infections.　Methods　 The relevant bacterial materials from 557 patients with general surgical infection from June 1993 to July 1997 were investigated.　Results　 1 045 strains in 38 species of gram-negative bacteria and 418 strains (8 species) of gram-positive bacteria were isolated with a negative over positive rate of 71.4% to 28.6%. For gram-negatives, E coli was the most common, and enterobactor cloacae ranking the third was on a dramatic increase from isolations. Staphylococcus aureus and staphylococcus epidemidis were the most common gram-positive species followed by enterococcus faecalis. Enterobactor cloacae and enterococcus faecalis are multi-antibiotic resistant bacteria. The result of drug susceptibility test showed that gram-negative bacteria were sensitive to fluoroqinolones or third-generation cephalosporins while gram-positive bacteria were sensitive to vancomycin and rifampicin.　 Conclusion　 This study showed that the rational application of antibiotics is very important to suppress the spectrum changing and antibiotic resistance of the bacteria.

Objective　To assess fetal cardiac hypertrophy and ventricular function in fetuses of diabetic mothers. Methods　Study group was consist of 23 fetuses of diabetic pregnancies. Control group was consist of 108 fetuses of normal pregnancies. Cardiac size was measured by ultrasound as inter-ventricular septal (IVS) thickness and ventricular diastolic and systolic (VD and VS) dimensions and perimeter. Cardiac function was expressed as ventricular shortening fraction 1 (VSF1) (ventricular diastolic perimeter -ventricular systolic perimeter/ventricular diastolic perimeter)and ventricular shortening fraction 2 (VSF2) (ventricular diastolic dimension-Ventricular systolic dimension/ventricular diastolic dimension).Fetal birth weight was recorded. Results　 IVS of study group （6.1±0.7） mm, which was significantly thicker than IVS of control group （4.4±0.7） mm. Left ventricular diastolic dimension (LVDD) and Left ventricular systolic dimension (LVSD) of study group were （3.3±0.8）cm2 and （2.2±0.6） cm2, which were greater than those of control group （2.6±0.6） cm2 and （2.0±0.5） cm2. Right ventricular diastolic dimension (RVDD) and Right ventricular systolic dimension (RVSD) of study group were （3.9±0.7） cm2　and （2.6±0.7） cm2, which were also greater than those of control group （3.5±0.5） cm2 and （2.4±0.7） cm2. Left ventricular shortening fraction 1 of left ventricular (LVSF1) of study group was 0.20±0.07, which was greater than that of control group （0.12±0.04）. LVSF2 of study group was 0.35±0.11，which was greater than that of control group too （0.23±0.10）. Right ventricular shortening fraction 1 of left ventricular (RVSF1) of study group was 0.19±0.05，which was greater than that of control group （0.13±0.11）. RVSF2 of study group was 0.33±0.09，which was greater than that of control group （0.23±0.14）. Estimated fetal birth weight of study group was （4 076±608） g, which was greater than that of control group （3 440±377） g. Conclusion　These findings showed that fetal hyperinsulinemia result in fetal weight increasing, global cardiac enlarger inter-ventricular septal thickness larger and revealed hypercontractillty of ventricle in fetuses of diabetas mothers.

ObjectiveTo investigate the gene expression of Chk1 gene in cerebrum after brain ischemia-reperfusion,trying to provide evidence to elucidatethe molecular mechanism of brain injury. MethodsEighty-five male Wistar rats were divided into 3 groups. Group Ⅰ served as normal control. In group Ⅱ (non-ischemia group) animals underwent the whole experimental procedures except the occlusion of the bilateral vertebro-arteries and common carotid arteries. In group Ⅲ(ischemiareperfusion group) animals were further divided into 3 subgroups according to the duration of ischemia: 10min, 30min and 60min. Each subgroup was again further divided based on the duration of reperfusion: 30min, 2h and 6h. The cerebrum was immediately removed from rats after complete brain ischemiareperfusion. The RNA was isolated and the reverse-transcription-polymerase chain reaction (RT-PCR) was carried out . The cDNA was analyzed by automatic system and the parameters were assessed to define the status of Chk1 mRNA expression in different ischemia and reperfusion groups. ResultsThe quantity of Chk1 mRNA expression in the cerebral cortex of normal adult rat was about half of the glyceraldehyde-3-phosphatedehydrogenase. When reperfused for 30min, 2h or 6h following 10min, 30 min cerebral ischemia and reperfused for 30min or 2h following 60 min cerebral ischemia, the expression of Chk1 mRNA was not significantly different from that in non-ischemia group. Only reperfused for 6h following 60 min cerebral ischemia, Chk1 mRNA expression decreased significantly. ConclusionsThe results indicate that Chk1 gene might be involved in molecular mechanism of cerebrum damage during complete global brain ischemia- reperfusion.

Objective The object of this study was to characterize the theraputic method of Shangtian and Bobath in treating cerebral palsy.Methods Sixty four patients with cerebral palsy,age from ,were subjected to this study.They were divided into two groups.treated with Shangtian method and Bobath method.Quantity evaluation on motion function was performed before and after the treatment.Results The overall score on motion function development was not significantly different between two groups(P >0.05).However,These were significantly different in seating function development and induces protective extensor thrust parachute reaction,between the two groups.Positive suppoting reaction improvement of 73％ in shangtian treated group,62％ in Bobath's treated group. Conclusion Both of the two therapeutic methods are effective in treating cerebral palsy.However.Shangtian is more effectual in improving seating function,postive suppoting reaction and induced protective extensor thrust parachute reaction.

Objective To evaluate the efficacy of laparoscopic ovarian electrocautery in refractory polycystic ovarian syndrome (PCOS). Methods A total of 16 PCOS patients who had no response to ovulation-inductive therapy were treated by ovarian electrocautery via laparoscope. After surgery, the ovulation as well as response to ovulation-inductive drugs was observed. The serum concentrations of FSH, LH, T, E 2 before and after operations were respectively compared. Results All 16 cases were pathologically diagnosed as polycystic ovary. There were significant decreases in luteinizing hormone (LH) and testosterone (T) after operations (P

Objective:To investigate the influence of Bifidobacterium adolescence on the expression of HIF-1?and VEGF in rabbit VX2 tumor.Methods:Rabbit VX2 tumor models were established and were divided into two groups(n= 10).Animals in the experimental group were injected with Bifidobacterium adolescence and those in the control group received normal saline via ear vein.Animals were sacrificed and the liver,kidney,spleen,heart,lung and tumor tissues were removed,cultured and Gram stained.The changes of tumor tissue were observed by H-E staining;immunohistochemistry was used to detect the expression of HIF-1?and VEGF in tumor tissues.Results:Bifidobacterium adolescence could target the tumor tissue.H-E staining showed the necrotic area was obviously enlarged after treatment with Bifidobacterium. Immunohistochemistry demonstrated that the positive rates of HIF-1?and VEGF in the experimental group were significantly lower than those of the control group(P