Objective To compare the degrees of stress reaction among laparoscopic cholecystectomy(LC),mini-incision open cholecystectmy(MIOC) and conventional open cholecystectomy(OC).Methods Ninety patients with gallstone were randomly divided into three groups:(LC),(MIOC) and (OC).Blood Thyroxin(T 3),throid-stimulating hormone(TSH),cortisol,insulin,glucose,white blood cell and temperature were measured and/or recorded in the perioperative period.Results The stress rection in LC was significantly lighter than that in OC,but the stress reaction degree in LC and MIOC were basic equality.Conclusion The stress reaction in LC is small than those of OC.