Objective To explore the progess and toxicity of concocting of Xinjiang datura stramonium L seeds. Methods The concocting of Xinjiang datura stramonium L seeds was preparaed by uighur medicine concocted The mice were selected to carry out acute and subacute toxicity test for the concocting of Xinjiang datura stramonium.L seeds. Results The dose of the concocting of Xinjiang datura stramonium L seeds which mice tolerated was 32 g ( crude drug)/kg. Compared with the control group,the serum AST level of the concocting group increased at 2 d ( P < 0.05 ); At 7 d, 14d the serum ALT level in male mice increased ( P < 0.05, P < 0.01 ); At 2 d,7 d, 14 d the serum BUN level in female mice decreased (P <0.05 ～ P <0.01 ) ;At 2 d,7 d the serum CRE level in female mice decreased ( P < 0.05 ～ P < 0.01 ). Conclusion Uighur medicine concocting could decrease the toxicity of Xinjiang datura stramonium L seeds.