Objective To discuss influence of different disinfection solution on infection and pain of puncture hole in patients with open fracture of both tibia and fibula treated with external fixation.Methods 41 patients were divided into group A(21 cases)and group B(20 cases).Group A adopted 75% ethanol to sterilize the puncture holes.While the group B used 5%iodide instead.The incidence of infection and pain of puncture holes was observed separately at the 1st week,6th week after operation and removing of fixation pin.Results The incidence of infection of puncture holes was not apparently different from each other(P>0.05)at any other time points.The incidence of paint in group B was lower than group A at the 1st week and 6th week after operation(P<0.05).The incidence of pain was higher in patients with puncture hole infection than those without puncture hole infection(P<0.01).Conclusion No difference was seen in puncture hole infection with either 75% ethanol or 5% iodide.Infection of puncture hole was correlated with pain only at the 1st week after operation.Pain could be alleviated with application of 5% iodide.

Sodium Ferulate(SF)is sodium salt of ferulic acid.Experimental studies showed that SF can reduce ischemical reperfusion injury of brain,heart,and liver.Clinically,SF was widely used in cardio-cerebrovascular diseases and immune system disorder.In the article,the experimental studies on SF preventing ischemical reperfusion injury of tissues were reviewed.

Objective To explore the management mode for mother and child care service among floating population. Methods Nursing process was applied for assessment, diagnosis, plan, implementation and evaluation of management mode for mother and child care service among floating population in Shiqiao street, Xiacheng district, Hangzhou city. Results Compared with those three years before implementing the nursing process, themanagement of mother and child care was enhanced, for example, the record rate of pregnant woman increased by 208.82% and the systematic management rate increased by 32.70%, the record rate of child care increased by 931.73%, the systematic management rate of child care increased by 31.85%. Conclusions If used properly, nursing process can help to promote the management of mother and child care among floating population greatly.

Claudins ; Humans ; Inflammatory Bowel Diseases

Objective To investigate the cellular localization and developmental patterns of NMDA receptor subunits(NMDAR1 and NMDAR2A/B) in the ventral horn of rat spinal cord during postnatal stages. Method Immunohistochemical staining method was used in the present study. Results NMDAR1-or NMDAR2A/B-immunorectivity is abundantly observed in the lamina Ⅷ and Ⅸ of ventral horn.The immunopositive product is localized mainly in the neuronal somata,dendrites and axon terminals.Dynamic changes of NMDA receptor expression occur in early postnatal stages.NMDA receptor subunits express weakly at postnatal 7 days (P7),goes up at P14,reaches maximum level at P21,then remains this level until adult. Conclusion Neuronal functional NMDA receptors are composed of NMDAR1 and NMDAR2A in heteromeric configuration in the ventral horn of spinal cord;NMDA receptors may be involved in motor neuron maturation and plasticity during postnatal development.;

Objective To study the expression, purification and bioactivity of a recombinant fusion protein consisting of anti-HBs single chain Fv and interleukin-2. Methods The engineering bacterium M15[pQE-ScFv-IL-2] which can express the fusion protein consisting of anti-HBsAg single chain Fv and interleukin-2, was induced by IPTG, then a 43kD recombinant protein was identified by SDS-PAGE and Western-blot analysis. Results The ratio of target protein to total protein of host reached 18%. Further analysis confirmed that the recombinant protein formed inclusion body in the cytoplasm of bacteria. 95% purity could be achieved after two-step purification of ScFv-IL-2, including Ni metal chelating chromatography (the first) and ion-exchange chromatogram (the second). The bioactivity assay of the purified product showed that the antibody-cytokine fusion protein could bind to HBsAg specifically and stimulate the proliferation of CTLL-2. Conclusion These results suggest that the fusion protein retains the bioactivity of its parental molecules, and may be a potential gene-engineering targeting drug for the treatment of chronic hepatitis B and other relevant diseases.

Objective To study the expression in high density fermentation of anti HBsAg Fab fragment in Pichia pastoris , and the purification and activity detection of expressed target protein. Method The high density fermentation of genetically engineered Pichia pastoris was proceeded in a 5L bioreactor using fed batch fermentation. The fermentation temperature was set at 28-30℃,the pH was 5 0-5 5, and the DO was kept over 20%. When the absorbance (OD 600 ) of the broth reached 400-450 (the first time of fermentation), 200-250 (the second time), and 300-350 (the third time), the induced phase was initiated, and the methanol concentration was 0 5%-1%. The fermentation ended after 96h's induction, the target protein was purified by affinity chromatography, and its activity was assessed by ELISA. Results It showed that the optimum initial cell density during methanol induced phase should be 300-350, which was good for control of the fermentation process and the expression of recombinant Fab. At the end of the fed batch phase, a yield of about 245mg/l of Fab was reached, and 98% purity could be reached as demonstrated by affinity chromatography. The results of ELISA showed that the supernatant of fermentation and the purified recombinant Fab could bind to HBsAg specifically. Conclusion The success of high density fermentation lays a sound foundation of mass production and clinical applications of recombinant humanized anti HBsAg Fab fragment

Objective The aim of the study was to develop a purification procedure on Pichia pastoris GS115/Fab expressing human anti-HBsAg Fab fragment. Methods Purity and yield ratio and conjugated activity of purified Fab fragment were analyzed with three purified ways of goat anti human Fab affinity chromatography and 14F7 monoclonal antibody affinity column, as well as Ion exchange Size exclusion column. Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. ForIon exchange Size exclusion column, purity and yield ratio of Fab fragment were very good, being 93.8% and 80% or more, respectively. Results of ELISA analysis showed that purified Fab fragment through three columns could bind to HBsAg specifically. Conclusion The purification process of recombinant anti-HBsAg Fab fragment was established. It lays a foundation for industrialization and clinical research of human anti-HBsAg Fab fragment.

The activities of interleukin 6 (IL 6) and tumor necrosis factor alpha (TNF?) produced by peripheral blood mononuclear cells (PBMCs) in vitro and their relevant levels in sera were measured in 49 patients with psoriasis vulgaris (PV) and in 20 normal controls using bioassay. The relationship between them was also analysed. The results showed that in PV patients the activites of IL 6 and TNF? produced by PBMCs and their serum levels were higher than those in healthy controls. There was a positive linear correlation between the activites in vitro and relevant levels in sera, and a positive linear correlation between serum levels of IL 6 and TNF?. These results indicate that there is dysfunction of cellular immune of PBMCs in PV patients. The increase of serum levels of IL 6 and TNF? may be related to the increased activity of PBMCs, and there may be a synergic action between IL 6 and TNF? in the pathogenesis of psoriasis.