OBJECTIVETo explore the expressions and functions of the kisspeptin/kiss1r system and GnRH in the hypothalamic arcuate nucleus (HAN) and the influence of the kisspeptin/kiss1r system on the hypothalamic-pituitary-testis (HPT) axis in the rat models of diet-induced obesity.

METHODSNinety newborn SD male rats were randomly assigned to receive normal diet (n = 30) and high-fat diet (n = 60) for the establishment of obesity models. The model rats were again equally divided into a control group and an experimental group, the latter injected with kisspeptin via the lateral ventricle. Then the body mass index (BMI) and endocrine hormone levels of the rats were recorded, the protein expressions of LepR, kisspeptin, kiss1r, and GnRH in the HAN determined by immunohistochemistry and Western blot, and the levels of GnRH mRNA in the HAN measured by qRT-PCR.

RESULTSSignificantly increased BMI and hormone levels indicated the successful establishment of diet-induced obesity models. Compared with the normal rats, the protein expressions of LepR, kisspeptin, and GnRH in the HAN were markedly decreased in the controls, and that of GnRH and the levels of LH and T significantly increased, but the expressions of LepR and kiss1r showed no remarkable changes in the experimental rats.

CONCLUSIONLateral ventricular injection of kisspeptin can upregulate obesity-induced low expression of GnRH, correct the dysfunction of the HPT axis, and thus improve reproductive function in rats.

Animals ; Arcuate Nucleus of Hypothalamus ; metabolism ; Diet, High-Fat ; Disease Models, Animal ; Female ; Gonadotropin-Releasing Hormone ; metabolism ; Hypothalamo-Hypophyseal System ; Kisspeptins ; metabolism ; Male ; Obesity ; metabolism ; Pregnancy ; Rats ; Rats, Sprague-Dawley ; Receptors, G-Protein-Coupled ; metabolism ; Receptors, Kisspeptin-1 ; Receptors, Leptin ; metabolism

This paper briefly introduced the characteristics, origin and development of virtual reality, and the value for rehabilitation; reviewed the application of virtual reality in the rehabilitation of balance function after stroke. Some assessment commonly used for balance function were summarized.

TED-Med Viewing, Listening&Speaking is compiled to serve academic audio-visual-oral English teaching in medical colleges. This paper elaborates some compilation ideas concerning this text-book. First, both the urgent needs of academic English teaching and the unique advantages of TED speech are the major stimulus to compiling this textbook. Second, its compiling philosophy adheres to learning centered, meaningful input. Third, compiling process is divided into three stages-preparation, writing and evaluation. Third, compiling principles includes four aspects: language and medical knowledge integrate
organically, the text and the task are authentic; proficiency training approaches are scientific; and the text-book is suitable for autonomous learning. Forth, the main content covers four medicine-related themes-humanity, health, disease, science and technology; each theme consists of three sub topics with 12 units (topics) in total; the content is interesting, informative and reflective. These compilation ideas prove to be scientific and feasible demonstrated by English teachers and medical experts. TED-Med Viewing, Listening&Speaking is expected to be completed and published earlier in order to promote academic English teach-ing and research in medical colleges.

Objective To investigate the risk factors of wound complications following the abdominal aorta balloon occlusion in order to provide the basis for the preoperative management and reduce postoperative complications.Methods Two hundred and sixty-one patients with the sacral tumor aged over 18 were selected as subjects who hospitalized at Peking University People's Hospital from January 2006 to December 2012.All subjects were underwent sacral tumor posterior resection operation after occlusion of the distal abdominal aorta.Patients were divided into two groups according to the existence of postoperative wound disorder.The general information of the patients was collected including intra-operative data and post-operative data.SPSS19.0 software was applied for statistic analysis Results Among 261 cases,89 cases were with incision complications,and the incidence of complications was 34.1％ (89/261).There was no significant difference regarding of age,weight,preoperative complications (P ＜ 0.05).Compared with that of no complications group,operation time of wound complications group was longer ((218.27 ± 72.08) min vs.(197.40 ± 53.92) min,P =0.046),and intra-operative bleeding was more ((2368.0 ± 1984.7) ml vs.(1701.6 ±958.8) ml,P =0.000),as well as a longer balloon occlusion time ((78.0 ± 23.8) rain vs.(65.2 ± 17.8) min,P =0.005).Moreover long hospitalized periods was in wound complications group ((47.6 ± 26.3) d,vs.(26.7 ± 9.0) d,P ＜ 0.05).Single factor analysis showed that the factors including operation time,intra-operative bleeding volume,time and times of balloon occlusion balloon occlusion were related to postoperative wound complications.Non conditional logistic regression analysis shows that factors of the balloon occlusion time(OR 1.028,95％ CI 1.012-1.045,P =0.001) and block number(OR 6.266,95％ CI 1.186-33.119,P =0.031) were related to the wound healing following balloon occlusion the abdominal aorta.Conclusion The risk factors of wound complications following the abdominal aorta balloon occlusion included occurrence balloon occlusion time and blocking times.That single balloon occlusion with 60 min reduced wound complication rate.

Objective:To evaluate the single dose and dosage frequency of analgesic drugs in 9 Chinese cancer hospitals in 2012. Methods:Using a retrospective method, the utilization of analgesic drugs in 9 cancer hospitals in the year of 2012 was analyzed statis-tically. Results:Most of single dosage of 15 analgesic drugs was consistent with the package inserts except for flunarizine and pethidine oral preparations with single overdose up to 50%. Most of dosage frequency of 21 analgesic drugs was consistent with the package in-serts except for oxycodone/acetaminophen, dihydrocodeine/paracetamol and dezocine with inconsistent dosage frequency ratio up to 30%. Conclusion:Most of single dose and dosage frequency of analgesic drugs is consistent with the package inserts. There is some unreasonable usage that should be paid attention to. Clinicians should be more cautious in analgesic drug use to make correct and ra-tional decision on dose and frequency, meanwhile, drug use in patients should be monitored to ensure the medication safety.

Objective To summarize experience of periopertively nursing children with floating thumb treated with composite tissue flap transplantation. Method Health education was done to 5 children with floating thumb treated with composite tissue flap grafting as well as their families including preparing preoperational checks, preventing crisis of blood circulation, medication and pain nursing and paying attention to rehabilitation instruction. Result All thumbs survived successfully and one patient developed with crisis of blood circulation, which disappeared in 15 minutes. Conclusion Good preoperative nursing and effective health instruction are critical for nursing of the children with floating thumb treated with composite tissue flap transplantation.

Objective To study the effectiveness of comprehensive rehabilitative therapy with paraplegic spinal cord injury (SCI) patients. Methods Forty patients with SCI-caused paraplegia were divided randomly into a rehabilitation group and a control group. The routine treatment was administered to both groups, and comprehensive rehabilitative therapy was also administered to patients in the rehabilitation group as an addition. Functional assessments and somatosensory evoked potential (SEP) tests were performed with the two groups pre-treatment and 30 days post-treatment. Results The rehabilitation group achieved, on average, greater improvement in their physical functions , as demonstrated by their much higher scores in terms of the Barthel index than those of the control group ( P

OBJECTIVE: To investigate the protective effect of Zingiber officinale extract on retinal ischemia-reperfusion (RIR) injury in rats. METHODS: 45 SD rats were randomly divided into normal control group, model group and Z.officinale extract group (400 mg?kg-1).RIR models were induced by infusing normal saline into anterior chambers for l hour. During five days before modeling,Z.officinale extract group were oral administration of Z.officinale extract two times per day by i.g. and model group distilled water. The medication regime remained after modeling. The activity of SOD and the content of malonyldialdehyde(MDA) and NO in retina were measured at 12 h,24 h,48 h and 72 h after modeling. The retinal ultrastructural changes were detected under the electron microscope. RESULTS: Compared with model group, in high-dose of Z.officinale extract group Z.officinale extract could strengthen the activity of SOD, reduce the levels of MDA and NO(P

Objective To investigate the clinical effect of Salvia Ligustrazine Injection in the treatment of hypertensive intracerebral hemorrhage.MethodsFrom February 2013 to May 2016, 118 patients with hypertensive intracerebral hemorrhage admitted in our hospital were randomly divided into Danshen ligustrazine group, conventional group and 59 cases.2 groups of patients were treated with the same basic treatment after surgery, Salvia Ligustrazine group also received Danshen ligustrazine injection, compared the two groups after treatment of clinical effects, nerve defect function recovery.ResultsAfter 14 days of treatment, the Glasgow Coma Scale (GCS) GCS score, neurological deficit score, C reactive protein (CRP) and ET-endothelin-1 were lower in the Salvia Ligustrazine group (13.56%), brain edema in grade 1 (83.05%), 2 cases of brain edema in grade 2 (3.39%), and 9 cases of cerebral edema in grade 2 (79.66%), 9 cases (15.25%) in grade 2 brain edema, the difference was statistically significant (P<0.05), and the treatment group was significantly higher than that in the control group After 3 months, the prognosis of patients with Salvia miltiorrhiza was good (49.05%), and the prognosis was good in 39 cases (66.10%), the difference was statistically significant (P<0.05).ConclusionSalvia Ligustrazine Injection is an effective method for the treatment of hypertensive intracerebral hemorrhage in patients with cerebral edema as soon as possible.It can promote the recovery of neurological function and benefit the patients' long-term recovery.

Adrenergic beta-Agonists ; therapeutic use ; Aged ; Androstadienes ; therapeutic use ; Cholinergic Antagonists ; therapeutic use ; Fluticasone ; Humans ; Male ; Middle Aged ; Scopolamine Derivatives ; therapeutic use ; Silicosis ; drug therapy ; Tiotropium Bromide