Objective To investigate the clinical effect of Salvia Ligustrazine Injection in the treatment of hypertensive intracerebral hemorrhage.MethodsFrom February 2013 to May 2016, 118 patients with hypertensive intracerebral hemorrhage admitted in our hospital were randomly divided into Danshen ligustrazine group, conventional group and 59 cases.2 groups of patients were treated with the same basic treatment after surgery, Salvia Ligustrazine group also received Danshen ligustrazine injection, compared the two groups after treatment of clinical effects, nerve defect function recovery.ResultsAfter 14 days of treatment, the Glasgow Coma Scale (GCS) GCS score, neurological deficit score, C reactive protein (CRP) and ET-endothelin-1 were lower in the Salvia Ligustrazine group (13.56%), brain edema in grade 1 (83.05%), 2 cases of brain edema in grade 2 (3.39%), and 9 cases of cerebral edema in grade 2 (79.66%), 9 cases (15.25%) in grade 2 brain edema, the difference was statistically significant (P<0.05), and the treatment group was significantly higher than that in the control group After 3 months, the prognosis of patients with Salvia miltiorrhiza was good (49.05%), and the prognosis was good in 39 cases (66.10%), the difference was statistically significant (P<0.05).ConclusionSalvia Ligustrazine Injection is an effective method for the treatment of hypertensive intracerebral hemorrhage in patients with cerebral edema as soon as possible.It can promote the recovery of neurological function and benefit the patients' long-term recovery.

Cardiomyopathies ; classification ; diagnosis ; etiology ; Cardiomyopathy, Dilated ; diagnosis ; etiology ; Cardiomyopathy, Hypertrophic ; diagnosis ; etiology ; Child ; Humans ; Pediatrics ; Suggestion

Objective To investigate the pharmacokinetics of epidural ropivacaine in patients with liver dysfunction.Methods Twenty patients aged 20-58 yrs weighing 45-73 kg scheduled for upper abdominal surgery under general combined with epidural anesthesia were divided into 2 groups (n = 10 each) : group Ⅰ patients with obstructive jaundice and group Ⅱ patients with normal liver function. Epidural block was performed at T8.9 interspace. 0.75% ropivacaine 2 mg?kg-1 (containing adrenaline 5 ?g?ml-1) was injected into epidural space over 2 min. General anesthesia was induced at 30 min after epidural ropivacaine with ?-hydroxybutyrate 60-80 mg?kg-1, remifentanil 2 ?g?kg-1 and atracurium 0.5 mg?kg-1. The patients were intubated and mechanically ventilated. Anesthesia was maintained with inhalation of N2O:O2 (1:1) and intermittent i. v. boluses of atracurium and remifentanil infusion when needed. Pinprick sensory level, onset of analgesia and degree of motor block (Bromage scale) were assessed. Blood samples were taken from central vein at 0, 10, 20, 30, 45, 60, 90, 120, 150, 180, 240, 360, 480, 720 min after epidural ropivacaine for determination of plasma concentration of ropivacaine (HPLC). Results The two groups were comparable with respect to F/M ratio, age, body weight, duration of operation, intraoperative blood loss and amount of fluid infused. There was no significant difference in onset time and height of sensory block and degree of motor block. The plasma ropivacaine concentration was significantly higher in group Ⅰ (patients with liver dysfunction) than in group Ⅱ during 180-720 min after epidural ropivacaine. The concentration-time curves in the two groups were fitted to two compartment open pharmacokinetic model. The t1/2? was significantly prolonged, AUC0-t was significantly increased, CL and K10 were significantly decreased in group Ⅰ as compared with group Ⅱ. Conclusions Liver dysfunction does not affect the sensory and motor block produced by ropivacaine within 30 min after epidural injection. The metabolism of ropivacaine is significantly slower in patients with liver dysfunction.

Fusion Proteins, bcr-abl ; genetics ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; genetics ; Male ; Middle Aged ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; etiology

Objective To evaluate the effect of CYP3A4*1G genetic polymorphism on the pharmacokinetics of levobupivacaine after epidural administration.Methods One hundred and eleven American Society of Anesthesiologists physical status Ⅰ or Ⅱ patients,aged 30-60 yr,weighing 56-79 kg,scheduled for elective lower extremity surgery under epidural anesthesia,were enrolled in the study.Blood sampies were collected from the central vein before anesthesia for detection of CYP3A4*1G genotypes by polymerase chain reaction-restriction fragment length polymorphism.The patients were divided into 3 groups according to the CYP3A4*1G genotypes:wild hemozygote (CYP3A4*1/*1) group (w/w group),mutation heterozygote (CYP3A4*1/*1G) group (m/w group) and mutation hemozygote (CYP3A4*1G/*1G) group (m/m group).An epidural catheter was placed at the L1,2 interspace,and 0.75％ levobupivacaine 1.8 mg/kg was injected.Thirty-four patients were randomly selected,and blood samples from the central vein were collected at0,10,20,30,45,60,90,120,180,240,360,480,840 and 1 440 min after administration for determination of plasma concentrations of levobupivacaine by high-performance liquid chromatography.The pharmacokinetic parameters were calculated.Results There were 42 cases in group w/w,59 cases in group m/w,and 10 cases in group m/m.The frequency of CYP3A4*1G variant allele was 35.6％ in the 111 patients underwent lower extremity surgery.There were no significant differences between the three groups in the plasma concentrations of levobupivacaine at different time points,elimination halflife,clearance,distribution volume,elimination rate constant,peak plasma concentration,time to peak plasma concentration or area under the concentration-time curve (P＞0.05).Conclusion CYP3A4*1G genetic polymorphism is not one of the genetic factors contributing to the individual variation in the pharmacokinetics of levobupivacaine after epidural administration.

Leukotriene B4 is a small metabolite of phospholipid in membrane through PLA2,5-LO, LTA4 hydrolase and other related enzymes. It is been synthesized in almost all kinds of tissue cells, then transported to outside of the cells, which may be separated from its synthesis. LTB4 shows complex bioactivities, especially in inflam-mation, immune system and plays an important role. Its functions are mediated by its different receptors on the membrane. Also, LTB4 interacts with other cytokines, and they form a network regulating functions of the body.

Legal clinic education originating in the United States, aims at educating students on legal practical skills, and plays an positive role in cultivating students' ability of integrating theory with practice and training their various practical vocational skills. The present Chinese training mode of health law specialty does not perform very well in training students' practical ability, while legal clinic education can better make up for some deficiencies. Therefore, this paper learns from the experience of American universities in their legal clinic education, such as the particular division of specialties, the development of outside-school clinics, taking pre-training seriously, and the unique evaluation mechanism. Correspondingly, we explore to construct a set of reasonable legal clinic education system in health law specialty, including the orientation of legal clinics, the double training system inside and outside school, the construction of teaching staff and the establishment of evaluation mechanism.

Objective To investigate the pharmacokinetics of different concentrations of levobupivacaine for lumbar epidural anesthesia.Methods Twenty ASA Ⅰ or Ⅱ patients of both sexes, aged 35-59 years and scheduled for elective radical resection of rectal or colon carcinoma under general anesthesia combined with epidural block, were randomly divided into 2 groups (n=10 each):group Ⅰ (receiving 0.75% levobupivacaine) and group Ⅱ (receiving 0.5% levobupivacaine). Epidural block was performed at L1-2 interspace. Group Ⅰ and Ⅱ received epidural 0.75% and 0.5% levobupivacaine 2 mg/kg (containing adrenaline 5 μg/kg)injected slowly over 2 min, respectively. And 30 min later, general anesthesia was induced with y-hydroxybutyrate 60-80 mg/kg and remifentanil 1-2μg/kg. Tracheal intubation was facilitated with succinylcholine 1-1.5 mg/kg and the patients were mechanically ventilated. Anesthesia was maintained with inhalation of nitrous oxide (N2 O) and O2 (1:1) and continuous infusion of remifentanil 0.01-0.1μg·kg-1·min-1 and intermittent intravenous boluses of atracurium. Sensory and motor blocks were assessed after epidural levobupivacaine. Blood samples were taken from the central vein at 0, 10, 20, 30, 45, 60, 90, 120, 210, 300, 420,540, 660 and 840 min, respectively, after epidural administration for determination of plasma concentrations of levobupivacaine by high performance liquid chromatography.Results The plasma concentration-time curves of levobupivacaine were fitted to a two-compartment open model in the two groups and there were no significant differences in the pharmacokinetic profiles between the two groups. The onset time of sensory and motor blocks was shorter and the duration of the two blocks was longer with 0.75% levobupivacaine as compared with 0.5%levobupivacaine. The incidences of nausea and vomiting and hypotension were low and no severe cardiovascular and neurological side-effects developed.Conclusion The pharmacokinetic parameters do not differ significantly between epidural 0.75% and 0.5% levobupivacaine when the total doses are the same. And epidural anesthesia with either 0.75% or 0.5% levobupivacaine is safe.

[ABSTRACT]AIM:Toinvestigatetheroleofcysteine-rich61(Cyr61/CNN1)inproliferationandmigrationof bone marrow mesenchymal stem cells ( BMSCs ) .METHODS: The lentiviral vector carrying CCN 1 ( Lenti-GFP-CCN1 ) was constructed and then transfected into the rat BMSCs .The cells were divided into non-transfection group , transfection group ( transfected with Lenti-GFP-CCN1 ) and negative control group ( Lenti-GFP ) .The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope .The effects of CCN1 on the proliferation and mi-gration of BMSCs were detected by MTT assay and scratch wound healing assay .RESULTS:The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group .The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05).CONCLUSION:Exogenous CCN1 promotes the migration of BMSCs.