Objective:To investigate the protection mechanism of adenosine A1 receptor agonist(2-chloro-N6-cyclopentyladenosine, CCPA)delayed preconditioning for rabbit myocardium during ischemia reperfusion.Methods:Thirty New Zealand male white rabbits were randomly assigned to Sham group,I/R group and CCPA group,with ten in each.Group CCPA was given CCPA 0.1 mg/kg before myocardial ischemia.Twenty-four hours later the I/R and CCPA underwent 40 min of coronary occlusion followed by 2 h of reperfusion.Blood samples were taken from arterial line at 20 min before occlusion(T)1,20 min after occlusion(T2),40 min after occlusion(T)3,1 h after reperfusion(T)4and 2 h after reperfusion(T)5for determination of the plasma levels of IL-10.At the end of the reperfusion,infarct size(IS)and area at risk(AAR)were defined by Evans blue and TTC staining.The heart was harvested and levels of the HSP70 were determined by Western blot analysis,and ultrastructures were observed under the electron microscopy.Results:The IL-10 and HSP70 levels of group CCPA was higher than those of group I/R.The CCPA(P

Objective:To explore the efficiency of comprehensive sedation nursing in MRI examination to the epilepsy children underthe age of 4.Methods:180 children were randomly divided into the test group and the control group.The test group was given comprehensive sedation nursing,while the control group was only given the simple drug sedation by oral medicine called 10%chloral hydrate.The effective rate,the time children fell to sleep after given the drug and one-time success rate were recorded and analyzed between the two groups.Results:The effective rate,the time children fell to sleep after giving the drug and one-time success rate all have siganificant difference between these two groups(P

To create a novel kind of orthosis to correct the dysfunction of antebrachium, wrist, metacarpus and digit and to facilitate the protection and functional training after injury of forearm and hand. The orthosis is constituted of a cardinal shelf, a stem shelf of metacarpophalangeal joint, a front bracket, finger ferrules, elastic strings and steel wires. The cardinal shelf is composed of a backplate for dorsal palm and a bracket for wrist with a U-shaped rack fixed in front of it. Ferrules of index finger, middle finger and fourth finger and little finger are connected to the backplate on the cardinal shelf through elastic strings. The thumb ferrule is set outside the ferrule of index finger on the backplate with steel wires. Another ferrule that is used to enclose the wrist and antebrachium is linked to the crossbar of the U-shaped rack through elastic strings. This orthosis plays an effective role in the protection of nerves, tendons, extremity of vascular anastomosis and the prevention of allotopia after fracture due to different trainings. It can improve the range of motion of wrist joint, metacarpophalangeal joint, and articulationes interphalangeae and strengthen the muscle power of pronator teres, pronator quadratus, supinator, wrist extensor, wrist flexor, extensor digitoium, perforans manus and musculus flexor digitorum sublimes. This orthosis could be applied for patients after the operation of antebrachium,wrist and manus to promote the recovery of their functions.

Objective:To study anti-tumor activity of Xidan Suspensions in vivo.Methods: The mice were transported by armpit or intraperitoneum injection of H22,EC or P388 tumor cells.Results: Xidan Suspensions inhibited markedly the growth of H22,EC or P388 tumor cells(P

AIM: The fingerprints of Acanthopanax Senticosus(Puper.et Maxim) Harms was studied by microemulsion electrokinetic chromatography(MEEKC) in comparison with micellar electrokinetic capillary chromatography(MEKC) and capillary zone electrophoresis(CZE). METHODS: The electrophoretic electrolyte was composed of a 1.0 %(w/w) SDS,6.6 %(w/w) n-butanol,0.7 %(w/w) n-heptane,91.6 %(w/w) 25 mmol/L borate solution(adjusted pH 9.5 with NaOH solution). RESULTS: The MEEKC-FP had good precision,stability and reproducibility with the relative standard deviations(RSD) of the relative migration time less than 3.4 % and the RSD of the relative peak area was about 6.3 %. CONCLUSION: This method provides a standard for the better quality controlling of Acanthopanax Senticosus(Puper.et Maxim) Harms.than MEKC and CZE.

Objective:To explore the enhancement effect of two different bed-moving modes and their relative parameters in children's chest CT checkup.Methods:The enhancement of out-bed group was studied with the in-bed group as control and the mean data of the major parameters were acquired.Then the enhancement effect and the major parameters were analyzed to make clear the differences presented in the two groups.Results:No difference was found between the two groups in enhancement effect and the data of major parameters.Conclusion:For each bed-moving mode,enhancement effect relied on the reasonable determination of the integrative parameters,but not a single one.

Objective:To study the role of AQP9 and its mRNA expression in the formation of ischemic cerebral edema in the rats.Methods:MCAO models were established by occluding unilateral middle cerebral artery(MCA) of the rats with the suture method.The morphological changes were observed with HE stain and transmission electron microscope(TEM).Brain water content(BWC) was measured by using wet-dry weighing method.The expression of AQP9 and its mRNA in the edema brain tissue were detected with in situ hybrilization(ISH) and immunohistochemistry(IHC).Results:The space around the neurons and perivascular space became larger.Some neurons showed necrosis phenotype,and the number of the neurogliocyte increased.The expression of AQP9 and its mRNA were up-regulated in subfornical organ(SFO)、choroid plexes、brain cortex、supraoptic nuclei、hippocampus and other regions,and reached its peak at 48~72h after MCAO.The brain water content(BWC) in ischemic cerebral tissue reached its peak at 72h.Conclusions:the expression of AQP9 and its mRNA were up-regulated following the brain edema.The results imply that AQP9 might play an important role in the formation of ischemic cerebral edema.

Objectives: To find out influencing factors of quality of life(QOL) of staffs in Centers for Disease Prevention and Control(CDC),whose results would be taken as theoretical evidence for government policy-makers to work out strategy of health human resources.Methods: The WHO Quality of Life Brief Scale(WHOQOL-BREF) was employed as the assessing tool for staffs of CDC who were in a city and its neighborhood counties.Each subject was asked to complete a spot self-assessment.Results: There is no significant difference of QOL of staffs between city and neighborhood county.Working at different departments was one of the influencing factors on psychological and social domains.Harmonious family relationship,good appetite,agreement to work competition,and confidence in working institutes were influencing factors to the total score of QOL.Conclusions: Scores of QOL of CDC staffs were relatively low with various influencing factors.It is necessary for leaders of related departments to take countermeasures to improve the QOL of CDC staffs.

AIM: To introduce a new kind of wrist-hand orthosis.METHODS: The major frame of wrist-hand orthosis is composed of dorsal carpal guard plate and carpal bracket; while, a U-shaped anterior bracket is fixed on the anterior side of the major frame. Ferrules of index finger, middle finger, fourth finger and little finger respectively connect to dorsal carpal guard plate of the major frame via elastic brace; ferrule of thumb is fixed on one side of ferrule of index finger by using steel wires; an abduct elastic sleeve is designed on the lateral dorsal carpal guard plate of ferrule of little finger by using steel wires. According to experimental demands, wrist with the palms upward or downward puts into the major frame, which is composed of dorsal carpal guard plate and carpal bracket. The hand is anterior to the major frame; ferrules are located over rolling sleeve by forward and fingers are located below the rolling sleeve by forward; ferrules are respectively rang the index, middle, fourth and little fingers; ferrule of thumb is rang the thumb. Continuously, wrist rotates based on orthopaedic demands to realize various orthopaedic functions.RESULTS: The wrist-hand orthosis is used to help the hand maintain extension after extensor tendon injury operation in order to restrict active areas of fingers and promote healing of injured side. On the other hand, this new orthosis is also used to help wrist joint and digital joint maintain flexion after extensor tendon injury operation in order to improve flexion and extension of wrist joint, enlarge active area of flexion of wrist joint, enlarge drifting active area of radialis of wrist joint, and increase muscle strength of radial flexor muscle, ulnar flexor muscle, extensor carpi radialis muscle, extensor carpi ulnaris muscle, extensor carpi ulnaris muscle, flexor carpi ulnaris muscle, extensor digitorum communis muscle, superficial flexor muscle and deep flexor muscle.CONCLUSION: The wrist-hand orthosis refers to protection of wrist and hand after operations of nerve, tendon, muscle and vessel injuries and recovery of motor dysfunction of wrist joint and digital joint induced by wrist-hand fracture; on the other hand, this new orthosis can improve active area of wrist joint and strengthen muscle force.

In medical researches,large-scale collaborations spanning institutions and disciplines have emerged a key pattern for tackling major research subjects.Contract writing for medical research collaboration,as a legal means to address the rights and obligations of the parties,should be fair,reasonable,scientific and strict as much as possible.The author probed into problems found in current writing of medical research contracts,and came up with corresponding remedies.