Objective To define the expression of AQP4 in normal rat eyes at transcriptional and translational level, so as to provide morphological bases for the mechanisms of water transport in eyes. Methods Immunohistochemical SABC method and in situ hybridization were used to detect the expression and localization of AQP4 in normal rat eyes. Results The corneal epithelium, the iridal pigment epithelium cells, the nonpigment epithelium cells of ciliary body, the lens epithelium cells, the lens fiber cells, the outer nuclear layer cells, and the inner nuclear layer cells, the ganglion cells of the retina showed positive immunoreactivity of AQP4. The reactive substance was distributed in membrane but not in nuclei of all positive cells. The resuIts of in situ hybridization were identical with that of immuohistochemistry method. AQP4 mRNA could be detected in the cytoplasm of positive immunoreactive cells, however, nuclei were negative. Conclusion The results implied that AQP4 could coordinate with other AQP4 to play an important role in the secretion and regulation of aqueous humor, the maintenance of the diaphaneity of cornea and lens, the motivation and transmission of optesthesia.;

OBJECTIVE To study the therapeutic effect of ceftazidime(CAZ) in a rat model of pneumonia caused by CTX-M-14 extended-spectrum ?-lactamases(ESBLs)-producing Klebsiella pneumoniae which is susceptible to ceftazidime in vitro.METHODS Forty eight rats were divided into four groups randomly which were ceftazidime-treated group,piperacillin/tazobactam-treated group,ceftaxime-treated group and the control group.All rats were intranasally inoculated with bacterial suspension of CTX-M-14 ESBLs-producing K.pneumoniae to produce pneumonia models.Twenty four hours after inoculation,rats were intramuscularly administered ceftazidime,piperacillin/tazobactam,ceftaxime,or saline,respectively.The temperature,leucocyte count,percentage of neutrophils,and bacterial counts in the right lungs of rats were detected at some timepoints.RESULTS Compared with the ceftaxime-treated group and the control group,the leucocyte count,percentage of neutrophil count and common logarithm of the bacterial count in the right lungs of the ceftazidime-treated group and piperacillin/tazobactam-treated group after the treatment which lasted seventy two hours were significantly lower than those of ceftaxime-treated group and the control group(P0.05).CONCLUSIONS Ceftazidime shows good therapeutic effect in pneumonia in rats caused by CTX-M-14 ESBLs-producing K.pneumoniae which is susceptible in vitro.

This paper briefly introduced the characteristics, origin and development of virtual reality, and the value for rehabilitation; reviewed the application of virtual reality in the rehabilitation of balance function after stroke. Some assessment commonly used for balance function were summarized.

OBJECTIVETo observe the efficacy of encephalopathy therapeutic tongue acupoint apparatus (ETTAA) in the treatment of autism.

METHODSEighty-four children of autism were randomly divided into a tongue acupuncture group (group A) and a conventional training group (group B), 42 cases in each group. The behavior training and sensory integration training were carried out in group B and the ETTAA was added in group A. The apparatus was switched on for 20 min every time and 3 times a day. Treatment of two months were carried out in both groups. The score of childhood autism rating scale (CARS) and clinical efficacy in both groups were observed before and after treatment.

RESULTSAfter treatment, the CARS in both groups were significantly reduced (42.39 +/- 6.86 vs 32.15 +/- 5.12, P < 0.001; 44.58 +/- 6.76 vs 39.72 +/- 7.11, P < 0.05), which was more significant in group A (P < 0.01). The totally effective rate in group A [90.5% (38/42)] was superior to that in group B [66.7% (28/42), P < 0.01].

CONCLUSIONThe clinical efficacy in tongue acupuncture group is apparently superior to that in conventional training group, ETTAA combined with conventional training have a better curative effect in the treatment of autism.

Acupressure ; Acupuncture Points ; Autistic Disorder ; therapy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Tongue ; anatomy & histology

Language ; Periodicals as Topic ; Publishing

OBJECTIVETo analyze the status and papers information of Chinese Journal of Applied Physiology, and provide a guide for authors, readers and editing staffs.

METHODSWe retrieved papers of Chinese Journal of Applied Physiology from 2009 to 2013 from CNKI, and performed a statistical analysis of papers information contained in the text using bibliometrics method. Papers information such as document type, found support, author affiliation type, and discipline classification, etc. was analyzed.

RESULTSSeven hundred and forty two papers including 27 full English articles in total were published in Chinese Journal of Applied Physiology from 2009 to 2013. More papers were supported by national, provincial, and the other foundation, it was about 82.6% of all papers.

CONCLUSIONChinese Journal of Applied Physiology has stable manuscript resources, various discipline papers, and play an important role for development of applied physiology in China.

Bibliometrics ; China ; Periodicals as Topic

Objective The dynamic accumulation trend of secondary metabolite in cultivated Spatholobus suberectus Dunn at different growth time was studied to provide scientific basis for confirming its reasonable harvesting time. Methods Ethanol-soluble extractives were measured by the method recorded in Chinese Pharmacopoeia ( A) Appendix XA ( 2010 edition). The content of total flavonoids was tested by ultraviolet spectrophotometer. The contents of protocatechuic acid, catechin, and epicatechin in Spatholobus suberectus Dunn were determined by high performance liquid chromatography ( HPLC). Results The contents of ethanol-soluble extractives, total flavonoids and the major components such as protocatechui cacid, catechin and epicatechin in Spatholobus suberectus Dunn showed increasing trend with the prolongation of growth time. The changes of contents of alcohol-soluble extract, total flavonoids and epicatechin in samples collecting at different months in one year showed a parabolic trend, and the contents reached the peak values from July to September. Catechin content kept increasing continually. The content of ethanol -soluble extractives of Spatholobus suberectus Dunn aged 4-6 years met the requirements of the current edition of Chinese Pharmacopoeia when harvested from July to September. Conclusion It is preliminarily recommended that Spatholobus suberectus Dunn cultivated for more than 7 years is suitable for harvesting, but the optimal harvest period and the harvest month still need to be further studied.

OBJECTIVE To investigate the procedures and advantages of whole range quality control of disposable sterile medical appliance. METHODS By analyzing the data concerning whole range quality control of disposable sterile medical appliance from Oct 2002 to Oct 2005 in our hospital to summarize the experiences in this field. RESULTS During the last 3 years, the supply room in our hospital had administrated disposable sterile medical appliance 30 069 000 articles altogether, with the qualification ratio of 100%, and no adverse reaction was found; furthermore, the waste was reduced for no massed articles occurred; the cost accounting was raised and the error ratio during delivery was reduced too, the annual error ratio dropped form 0.003% to 0.00066%. CONCLUSIONS The whole range quality control of disposable sterile medical appliance can raise the administration quality, reduce the waste during delivery, and guarantee the disposable sterile medical appliance being used safely and effectively, also, it can provide accurate and useful data of cost accounting to concerned departments.

OBJECTIVE To analyze occupational harmful factors in department of disinfection and supply and take protective measures in pertinency.METHODS We analyzed every tache of work flow in the course of disinfection and supply,and found out the potential occupational harmful factors.Based on them,the easy and feasible protective measures were proposed.RESULTS There existed many occupational harmful factors in the course of disinfection and supply.Therefore,the working environment and occupational protection among staff should be improved.CONCLUSIONS Analysis on the peril of occupational harmful factors,enforcement of protective consciousness and establishment and implementation of operation rules and protective measures are important steps of occupational safety management for staff in department of disinfection and supply.

The chemokine fractalkine primarily involves in chemotaxis,adherence and inflammation.Recently it has been discovered owning the ability of inhibiting cell death in neurons and glial cells,as well as protecting central nervous system from toxic damage.Fractalkine reduces the toxicity to neurons and glial cells mediated by excessive Fas L,glutamate(Glu) or lipopolysaccharide(LPS).In vivo neutralization of endogenous brain fractalkine signal pathway increases inflammatory cytokines secretion and neuronal cell death induced by LPS.Fractalkine may achieve its neuroprotective property through influencing expression of pro-apoptotic and anti-apoptotic proteins,intracellular Ca2+ level and inflammatory cytokines secretion via protein kinase B(PKB)/Akt and extracellular signal-regulated kinase(ERK)/MAPK pathways.