Aims: The emphasis of this study is to generate new valuable bioproducts from non-toxic cleaning waste for environmental healing technology. Methodology and Results: Comparisons between different types of biofertilizer formulations and the field trial effectiveness were done. Results indicated that biofertilizer C contained the highest N value (1.8%) when compared with biofertilizers B and A, which only contained 1.7% and 1.4%, respectively. Biofertilizer A showed significant difference in the total count of yeast, mould, ammonia oxidizing bacteria and nitrate oxidizing bacteria compared to biofertilizer B and C. Meanwhile, biofertilizer C was found to be significantly different from others in Lactobacillus sp. and nitrogen-fixing bacteria count. Photosynthetic total count and Actinomycetes sp. were not noticed in all formulations tested. Conclusion, significance and impact of study: The findings of this study suggest that biofertilizer A is suitable to be used as a promotional biofertilizer in flower and fruit production, biofertilizer B can be used for a leafy crop, while biofertilizer C is good for the growth of roots and stem of plants.

A study was carried out to isolate Brucella melitensis using established bacteriological and PCR techniques in Brucella seropositive goats in farms in Selangor, Negeri Sembilan, Melaka and Pulau Pinang. Brucella melitensis was isolated from 7 of 134 reactors with the highest isolation from the vaginal swabs (57.14%) followed by the spleen (28.57%), uterine fluid (14.29%). No Brucella was isolated from the lymph nodes. PCR confirmed all the seven isolates as B. melitensis and isolates were phylogenetically related to other isolates from India, Iran, and Israel but most closely related to isolates from Singapore.