1.Ameliorative Effects of Aquilaria malaccensis Leaves Aqueous Extract on Reproductive Toxicity Induced by Cyclophosphamide in Male Rats
Redzuan Nul Hakim Abdul Razak ; Faridah Ismail ; Muhammad Lokman MD Isa ; Azantee Yazmie Abdul Wahab ; Hussin Muhammad ; Roszaman Ramli ; Raja Arif Shah Raja Ismail
Malaysian Journal of Medical Sciences 2019;26(1):44-57
Background: Cyclophosphamide (CP) is a widely used anti-neoplastic and
immunosuppressive agent that is associated with adverse side effects including reproductive
toxicity. Aquilaria malaccensis (AM) is a traditional medicinal plant which was reported to exhibit
high anti-oxidant and free radical scavenging properties. The present study was aimed to evaluate
the protective effects of AM leaves extract on sperm quality following toxic exposure to CP.
Methods: Forty-eight male Sprague Dawley rats were allocated into eight groups of six
rats (n = 6): control, CP only (200 mg kg−1), AM only (100 mg kg−1, 300 mg kg−1 and 500 mg kg−1)
and CP + AM (100 mg kg−1, 300 mg kg−1 and 500 mg kg−1). Animals were sacrificed after 63 days of
treatment and the sperm from the caudal epididymis was taken for sperm analysis.
Results: The body and the reproductive organs weight, sperm count and motility did not
differ between CP and other groups (P > 0.05). A significant increase (P < 0.05) in percentage of the
dead and abnormal sperm were seen in the CP alone treated group compared to the control group.
Co-administration of AM to the CP exposed rats significantly reduced the (P < 0.05) percentage of
abnormal sperm as compared to the CP only group.
Conclusion: Overall, the present results represent the potential of AM to protect against
CP induced reproductive toxicity.