1.HbA2 levels in normal, B-thalassaemia and haemoglobin E carriers by capillary electrophoresis
Hafiza Alauddin ; Malisa Mohd Yusoff ; RD Aidifitrina Khirotdin ; Azlin Hanim ; Raja Zahratul Azma ; Matthew Chong Kwok Thong ; Irwan Mohamad Ali ; Yeoh Zi-Ning ; Lailyvia Mohd Ishak ; Nur Rabiatul Adawiah Mohd Radzi ; Noor Hamidah Hussin
The Malaysian Journal of Pathology 2012;34(2):161-164
Objective: The capillary electrophoresis (CE) is a new system that utilizes the principle of electrokinetic
separation of molecules in eight electrolyte buffer-fi lled silica capillaries. In this study, we established
the normal ranges of haemoglobin A2 (HbA2) and haemoglobin F (HbF) levels for normal individuals
using this system and also the HbA2 level in β thalassaemia and haemoglobin E (HbE) individuals.
Materials and Methods: 154 samples from normal individuals, 218 samples from β thalassaemia
heterozygotes and 91 samples from HbE heterozygotes were subjected to high performance liquid
chromatography (HPLC) and CE analysis. Results: The normal ranges for HbA2 and HbF by CE
were 2.75% (SD 0.26%) and 0.03% (SD 0.24%) respectively, which were signifi cantly lower than
that of HPLC 2.88% (SD 0.25%) and 0.58% (SD 0.61%) (p <0.001). The HbA2 level for HbE
heterozygotes was 3.58% (SD 0.44%), which was signifi cantly higher than normal (p <0.001) but
lower than that of β-thalassaemia heterozygotes (p<0.001) and the true HbE level was 24.28% (SD
3.38%). Conclusion: The CE system provided a fully automated and high throughput system for
haemoglobin analysis. We established the normal ranges for HbA2 and HbF levels by CE. We also
determined the ranges for HbA2 in beta thalassaemia and HbE heterozygotes using this system.
Result Analysis
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