OBJECTIVE To investigate the drug resistance of 554 Enterococcus isolated in our hospital. METHODS The clinical isolates were identified and antimicrobial susceptibility tests were detected by VITEK-60 of Bio-Merieux of France. RESULTS Totally 554 Enterococcus strains were isolated,including 361 E. faecalis (65.2%),143 E. faecium (25.8%),22 E. gallinarum (4.0%),18 E. aviums (3.2%) and 10 other Enterococcus isolates (1.8%). For E. faecalis,the resistance rates to ampicillin and penicillin were lower than the average,(1.1% and 5.3%),which can be chosed as the first selective drugs in clinical therapy for E. faecium,the resistance rates to ampicillin and penicillin both were over 74%,they should be carefully used in therapy. but quinupristin/dalfopristin and linezolid were the best choice.Curing infections caused by the other Enterococcus except E. faecalis and E. faecium,linezolid should be the first selective drug in clinical therapy. CONCLUSIONS While curing infections caused by multi-resistant Enterococcus,doctors should choose proper therapy and according to difference of their drug resistance among Enterococcus spp,in order to prevent appearance of drug-resistant bacteria.

0.05),and was superior in group C than that in groups A and B(P0.05),and was superior in group C than that in groups A and B(P

OBJECTIVE To study the clinical distribution and the drug resistance of extended-spectrum ?-lactamases (ESBLs) producing Klebsiella pneumoniae and Escherichia coli. METHODS The clinical isolates were identified by VITEK-60 of Bio-Merieux of France. K-B agar diffusion method and confirmed test were used to detect the drug resistance of ESBLs-producing K. pneumoniae and E. coli. RESULTS The incidence of ESBLs was 52.8% in E. coli and 63.5% in K. pneumoniae. E. coli was mostly isolated from urine (22.1%). The second was isolated from pus/wound (11.7%). Majority strains were isolated from surgical and pediatric wards. K. pneumoniae was mostly isolated from sputum and throat swab (37.8%), 21.8% from neonate ward and 16% from child ICU. CONCLUSIONS To cure infections caused by ESBLs-producing K. pneumoniae and E. coli, imipenem is the best choice and piperacillin/tazobactam is the second choice.

OBJECTIVE To study the incidence of ?-lactamases and oxacillin-resistant Staphylococcus aureus and their drug resistance in our hospital. METHODS The clinical isolates were identified by VITEK-60 of Bio-Merieux of France. K-B agar diffusion method and confirmed test were used to detect the drug resistance of oxacillin-resistant S. aureus. RESULTS Among 175 S. aureus isolates, the incidence of ?-lactamases was 94.3% and the incidence of oxacillin-resistant S. aureus was 28.6%. Oxacillin-resistant S. aureus was mostly isolated from pus/wound (52.0%), then from sputum and throat swab (32.0%). CONCLUSIONS To cure infections caused by ?-lactamases-noproducing S. aureus, penicillin is the best choice. For infections caused by ?-lactamases-producing but oxacillin sensitive S. aureus, the first generation cephalosporins are the best choice, while curing infections caused by ?-lactamases-producing and oxacillin- resistanct, S. aureus nitrofurantoin and linezolid are the best choice. The resistance rate to vancomycin is zero. It should be carefully used in therapy.

Objective To investigate the incidence of postoperation constipation in patients who experienced thoracolumbar fractures screw fixation surgery.Methods A prospective cohort study was performed by investigating 99 cases who experienced thoracolumbar fractures screw fixation surgery.The patients were followed up for 1 month.The postoperative constipation relief situation was observed.Kaplan-Meier method was used to draw a plot of survival,Cox proportional hazards regression model analysis was adopted for single factor and multiple factors analysis and the prediction model of constipation relief after surgery was established.Results The trend of postoperative constipation relief of patients showed a trend of first increased and then decreased,the number of cases reached high peak on the second week,the constipation relief median time was (14.00±0.76) days.Cox multiple factors regression analysis showed that prediction function model of postoperative constipation relief was h(t)=[h0(t)]e(-0.826X1+0.353X2+0.381X3-1.404X4).Conclusions There was a high incidence of constipation in patients with thora-columbar fractures after surgery and it is difficult to relieve,clinical nurses should pay more attention to the influencing factors of postoperative constipation relief and help patients reestablish normal bowel movement as soon as possible.

Objective To investigate the change of Th22 cells in peripheral blood of patients with Primary sjogren’s syndrome (pSS)and evaluate clinical significance.Methods 37 patients with pSS from January 2014 to November 2015 were enrolled the study as the observation group.Then 37 healthy adults receiving check-up during the same period were selected as con-trol groups in accordance with the proportion of 1∶1.Flow cytometry was performed to evaluate the levels of Th22 cells in peripheral blood,and tenzyme-linked immuno sorbent assay (ELISA)was used to measure the serum IL-22 levels.Pearson analysis was performed to investigate the relationship between Th22 level and serum IL-22 level,C3,C4,anti-SSA,anti-SSB, ANA antibody,EULAR Sjogren's syndrome disease activity index (ESSDAI)score in observation group.Then the levels of Th22 cells and serum IL-22 were compared among different labial gland pathologic stage in patients with pSS.Results The levels of Th22 cells and serum IL-22 in the observation group were (2.53±1.56)%,718.6±176.8 pg/ml respectively,and significantly higher than (1.24±0.51)%,258.9±72.4 pg/ml in control group (P<0.05).Th22 cell level was positively related with the level of serum IL-22,anti-SSA,anti-SSB and ESSDAI score,and negatively related with the content of C3 and C4 (P<0.05).There were no significant differences in the levels of Th22 cells and serum IL-22 different labial gland pathologic stage in patients with pSS (P>0.05).Conclusion The levels of Th22 cells and serum IL-22 significantly in-creased in patients with pSS,and related to other inflammatory indexes and disease activity,so they may participate in the genesis and development of pSS.

Objective To clone human vascular endothelial growth factor 165(hVEGF_ 165 ),construct its eukaryotic expression and to study the expression of hVEGF_ 165 . Methods Human vascular endothelial growth factor cDNA was amplified by PCR method from the HL60 cells and cloned to expression vector pcDNA_3,constructed pCD-hVGEF_ 165 recombinant plasmid, then transformed to E.coli BL21(DE3)cell.Results The cloned cDNA was confirmed to be VGEF_ 165 cDNA. It was observed that the expression of human VEGF gene was detected distinctly 72h after transferring.Conclusions We successfully cloned and expressed hGEF_ 165 gene, which provided the further foundation of the model of VEGF transgenic animal and makes a basis for the further study in retinal neovascularization.

Objective To improve the quality of first aid,raise the working efficiency of nurses,make the management of endemic area crash truck more standard,scientific and simple and provide swift medical care to patients in emergency.Methods An endemic area crash truck is developed in accordance with the rule and regime of first aid item.Results Endemic area crash truck was given a national practical newtype patent and was carried out in hospitals.Conclusion Endemic area crash truck has a reasonable construction and can be locked by one time lock.It greatly shorten the time for handing over and counting by nurses,which elevates the efficiency of nurses and guarantee the management quality of first aid item.

Objective To explore the relation among the quality of the Paridis polyphylla and different species and growth conditions, and help to provide scientific foundation for introduction and cultivation of Paridis polyphylla.Methods The samples of Paridis polyphylla were collected by different varieties, different growth years, different harvest seasons and different altitude habitats. The content of saponins in Paridis polyphylla was measured by HPLC method according to the Chinese Pharmacopoeia 2015. The chromatographic column was ACQUITY UPLC? HSS T3 (3.0 mm× 100 mm, 1.8μm), the mobile phase was acetonitrile and water (gradient elution), with flow rate of 0.5 ml/min, detection wavelength was 203 nm and column temperature at 35℃.Results Total saponin content ranged from 1.35% to 3.89% among the varieties studied. The content of each components were listed as:Paris polyphylla Smith(PS) >Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz. (PY) >Paris polyphylla Smith var. chinensis(Franch.) Hara (PC). From 3 to 10 years old of PC, the longer the growth years made the higher the total saponin content. Furthermore, total saponin content of PC increased gradually with the altitudes rising from 400 to 800 meters. The total saponin content of PY harvesting in spring was much higher than that of other seasons.Conclusions The Results showed the importance for introducing and cultivating of Paridis polyphylla.

Objective Isolating and purifying the major allergens from Metapenaeus ensis and identifying its immunoreactivity for diagnostic application. Methods The proteins extracted from Metapenaeus ensis were purified by DEAE-52 ion-exchange chromatography and Sephadex G-100 gel filtration. The eluted peaks were detected by dot-ELISA,and the immunoreactivity of the purified allergens was detected by ELISA with the sera from patients allergic to shrimp. The molecular weights of the purified allergic proteins were detected by SDS-PAGE. Results Two major allergic proteins which have highly immunoreactivity in ELISA detection were purified from Metapenaeus ensis. The molecular weights of which were 36 and 68kDa,respectively. Conclusion The major allergens of Metapenaeus ensis purified by ion-exchange chromatography and gel filtration have effective immunoreactivity and can be used as specific antigen in ELISA reagent.