Objective To study the effects of neferine on the expression of glutathione S-transferase-pi in vitro and to explore the multi-drug resistance reversing mechanism of neferine.Methods 50% inhibition concentration(IC_(50)) of ADM on K562/A02 was determined by MTT method.The transcription of GST-? gene was detected by semi-quantitative RT-PCR and the expression level of GST-? was determined by Western blot after neferine treatment.Results Neferine remarkably enhanced chemosensitivity to ADM of K562/A02 cells.After neferine treatment in day 1,day 3 and day 5,the relative efficiency of K562/A02 to ADM was 9.6%,41.4% and 10.7%,respectively.Semi-quantitative RT-PCR showed that mRNA transcription of GST-? gene was significantly reduced(P

Objective To search for a way of treating chi1dren's idtopathic nephrotic syndrome (INS) hypoadrenocorticismand its significance. Meethods Thirty-nine patients (31 males and 8 females) with INS were randomly divided into twogroups: 21 patients in tera-peutic group (treated by prednisone, astragali and acanthopanax root) and 18patients in control group (Simply treated by prednisone). 1ml blood was respectively drawn from all thepatients at 8 o'clock (one time before treatment and one time after treatment), the serum cortisol wasdetermined by radioimmunoassay (RIA). Results In control group, the levels of serum cortisol still remained lower than the normal value aftertreatment and the levels after large dosages prednisone treatment were markedly lower than that beforetrea-tment. The values of serum cortisol returned to be normal after treatment in therapeutic group. Conclusion Astragali and acanthopanax root can restore the hypoadrenocorticism in children with INS.

According to the epidemic situation in Hunan province, COVID-19: Emergency response measure for GCP in Hunan Province under the first-level response of major public health emergencies (version 2.0) as the consensus is reached by experts with years of rich experience in the field of drug clinical trials. We hope this paper can provide reference for GCP practitioners.

Serum creatinine was determined by enzymatic method.99mTc-GFR was measured by 99mTc-DTPA dynamic renal imaging and considered as GFR marker in 210 males and 180 females with type 2 diabetes,eGFR was calculated by Cockcroft-Gault formula,MDRD equation7,abbreviated MDRD equation,modified MDRD equation for Chinese (c-7GFR4 and c-aGFR4),and CKD-EPI equation.They were analyzed by correlation,regression,Bland-Altman analysis and receiver operating characteristic (ROC) curve analysis.The correlation coefficients for Cockcroft-Gault formula,MDRD equation7,abbreviated MDRD equation,c-7GFR4,c-aGFR4,and CKD-EPI equation were 0.79,0.76,0.77,0.76,0.76,0.81 respectively.And the differences were-14.99,-18.85,-23.79,-25.85,-32.07,and-7.16,respectively.The area under ROC curves were 0.91,0.88,0.89,0.88,0.90,and 0.92,respeetively.Kappa values were 0.67、0.52、0.39、0.49、0.46、0.54respectively.The CKD-EPI equation seams to be the most accurate measurement among the six methods when the serum creatinine was determined by enzymatic method in Chinese type 2 diabetic patients.

Objective To observe the postoperative changes of diplopia and eyeball movement disorder in patients with orbital fracture. Methods The clinical data of 62 patients with reconstructive surgeries for orbital fracture were retrorespectively analyzed.The position,range of fracture and incarceration of extraocular muscles were determined by CT scanning.The eyeball movement and diplopia were examined and recorded pre-operation,3,6 and 12 months after operation,respectively. Results Among the 62 cases,23 were simple orbital wall fracture and the other 39 were complex orbital fracture.Eighteen of the patients with simple orbital wall fracture had diplopia and 19 had eyeball movement disorder of Ⅰ degree or Ⅱ degree before operation.Twelve months after operation,5 had diplopia ofⅠ degree or Ⅱ degree,and 5 had eyeball movement disorder of Ⅰ degree.For those with complex orbital fracture,36 had diplopia and 38 had eyeball movement disorder.Twelve months after operation,29 had diplopia(III degree diplopia in 6) and 22 had eye movement disorder.Among the 12 patients with complex orbital fracture who received operation within 3 weeks after trauma, only one had III degree diplopia,while 5 of the 23 with complex orbital fractures repaired 3 months after trauma had III degree diplopia. Conclusion Surgical management can effectively improve diplopia and eyeball movement disorder resulted from orbital fracture.Earlier treatment is superior to later treatment in regard with the effect of surgical intervention.

Objective To evaluate clinical value of MGFA classification and QMG score on pre-dicting late extubation after thymectomy for myasthenia gravis(MG).Methods Total of 61 patients with MG received extended thymectomy from January 2007 to February 2012 were enrolled.Patients were divided into two groups:normal extubation group contained the other 47 patients without pro-longed postoperative mechanical ventilation and delayed extubation group included 14 patients with prolonged postoperative mechanical ventilation.The following factors were evaluated:gender,age, weight,MGFA classification,QMG score,history of steroid hormones or anticholinesterase drugs be-fore operation,the function of liver and kidney before operation,preoperative electrolyte,preoperative hemoglobin content,etc.Receiver operator characteristic curve (ROC)was plotted,and the predictive value, sensitivity and specificity of preoperative MGFA clinical classification and QMG score predicting postoperative ventilation in MG were calculated.Results Fourteen patients(22.95%)de-veloped breathing support after the anaesthetic or endotracheal intubation again in 48 hours.the area under ROC curve(AUC)for preoperative MGFA clinical classification predicting postoperative ventila-tion was 0.723 in MG,it had the sensitivity of 78.5% and specificity of 63.8%.The AUC for QMG score predicting postoperative ventilation was 0.866,the QMG score threshold value of 8.5 had the sensitivity of 78.6% and specificity of 87.2%.Conclusion MGFA classification and QMG score can predict late extubation after thymectomy in patients with myasthenia gravis.

[Objective] To observe the effects of blood-activating and stasis-removing (BASR) herbs on the proliferation and apoptosis of gastric mucosa cells (GMC) in experimental chronic atrophic gastritis (CAG) rats. [Methods] CAG rat models were established with comprehensive method and then were given BASR herbs. Argyrophilic protein of nucleolar organizer regions ( AgNOR) count was carried out after silver nitrate staining. The expression of proliferating cell nuclear antigen (PCNA) was examined with immunohistochemical method and apoptosis was detected by in-situ cell-end labeling method. [Results] The number of AgNOR and the indexes of PCNA and apoptosis were increased in CAG model rats ( P

Epithelial ovarian cancer is the most lethal gynecologic malignancy, drug?resistance will eventually appear after re?peated intermittent chemotherapy. However, second?line chemotherapy only works on a few patients, new drugs with better efficacy and less side effects are needed. There are a number of promising results of molecular targeted therapy in recent clinical trials for patients with recurrent epithelial ovarian cancer, such as Bevacizumab, Olaparib, Pazopanib, etc. They have been demonstrated to improve progression?free survival of patients with platinum?resistant ovarian cancer, which might become a new strategy for such patients. In this review, we summarized the current status and progress of targeted therapy for recurrent epithelial ovarian cancer.

Studies were made on the effect of protoporphyrin in treating the acute liver injury induced by carbon tetrachloride ( CCl4 ) in rabbits and on the liver blood flow, biosynthesis of DNA and protein in liver of mice. The results showed that the protoporphy-rin was able to protect the liver remarkable as evidenced by his-tological studies. And it could inhibit the elevation of serum glut-amic-pyruvic transaminase ( SGPT ) , serum glutamic - oxaloacetic transaminase ( SGOT ) and serum ?-glutamyl- transpeptidase ( S?- GT)also. Protoporphyrin could regulate the metabolism of serum amino -acids in rabbits intoxicated by CCl4 as well. Furthermore,protoporphyrin can improve biosynthesis of DNA and liver protein and it may increase the blood flow in the liver of the mice.

Objective To investigate behavior,knowledge and attitude of cigarette smoking,and their relationship among different occupational population in Jiangsu Province.Methods Convenient sampling was used to interview 516 staff from medical institution,schools,government offices,public transportations and waiting rooms,restaurants and internet bars.The content of interview included current smoking,second-hand smoking,and related knowledge and attitude.Results Proportion of current smoking in male(51.2%)was higher than that in female(0.3%,P90%),while it's relatively lower in "second-hand smoking and exact related diseases"(