OBJECTIVE: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome. METHODS: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected. RESULTS: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05). CONCLUSION: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Hemophilia is a common pediatric hereditary bleeding disorder,including hemophilia A (FⅧ deficiency) and hemophilia B (FⅨ deficiency).Children with hemophilia tend to spontaneous bleeding or bleeding after minor injuries or surgery.In this review,we briefly discussed the preoperative diagnosis and classification of hemophilia,dynamic monitoring of FⅧ or FⅨ,dose of clotting factor and duration of treatment and some other perioperative issues when surgery is needed,to guide hemophilia children's surgery and postoperative rehabilitation.

Objective To explore of laparoscopic abdominal trauma used in the diagnosis and treatment of gastrointestinal tract injury.Methods 180 cases of abdominal trauma and gastrointestinal injury were randomly divided into three groups,A group of 60 cases was given conventional nonoperative treatment,B group of 90 cases was given laparoscopic exploration to surgical treatment,C group of 30 cases was open to probe the surgical treatment.The clinical effect of three groups was observed.Results After treatment,A group of recovered 32 cases,improvement in 16 cases,invalid in 12 cases,the total effective rate 80.0％,length of hospital stay (8 ±4)d,B group healed 58 cases,30 cases improved,invalid in 2 cases,the total effective rate 97.8％,length of hospital stay (8 ± 3) d,C group of 19 cases were healed,better 10 cases,1 case ineffective,the total effective rate 96.7％,length of hospital stay (15 ± 5) d,A,B and C group total effective rate differences were statistically significant (x2 =13.45,4.50,all P ＜0.05).C group hospitalization time was longer than that in A,B group (t =5.24,3.34,all P ＜ 0.05).Conclusion Laparoscopic exploration surgery for abdominal trauma and gastrointestinal injury has better diagnosis and treatment effect,which can reduce the time in hospital to improve the prognosis of patients.

Objective To explore the specific causes of common peroneal nerve injury, characteristics of such injured nerve's electrophysiological performance and its diagnostic value.Methods The clinical and neurophys-iological examination data of 7 patients with common peroneal nerve injury were retrospectively analyzed.Results The patients of common peroneal nerve injury cause for long time squatting down.Compared with the healthy side, potential amplitude of the deep peroneal nerve or the superficial proneal nerve of the injured side changes more obviously.On the average the amplitude fell by about 50% with the largest drop by 88%.EMC indicated that the muscle controlled by injured nerve has spontaneous potential,reduced recruitment and so on.Conclusion Long time squatting down was relatively rare cause for common peroneal nerve injury.Neural physiological detection could provide more specific positioning diagnosis and differential diagnosis for common peroneal nerve injury,had better clinical application value.

Objective To discuss the application value of multi- position radiography in the diagnosis for fracture of rib.Methods 98 cases with integrated data of thoracic trauma undergone multi- position view X-ray from 2007 to 2009 were retrospectively reviewed.Results The percent of fractures with orthophoria view is 65.4%.The percents of fractures with oblique position,tangential projection and spot film under the X-ray fluoroscopy are 20.6%、5.1% 、8.8%,while doesn' t find the A-P position,The total percent is 34.6%.Conclusion Multi-position radiography can elevate the diagnosis for fracture of rib and has application value.

Cardiomyopathy, Hypertrophic ; Humans ; Infant, Newborn ; Infant, Premature

Objective To investigate the expression of Alzheimer′s disease(AD) patients with serum CRP,IL-6,and analyze its clinical significance.Methods The clinical data of 85 patients with AD (AD group)treated in our hospital from March 2015 to June 2016 were retrospectively analyzed,and the data of 85 healthy adults were selected as control group.The differences of serum CRP,IL-6 levels,the mini mental state rating scale(MMSE) and the daily living ability rating scale(BI) score of the two groups were observed,and the correlation between CRP,IL-6 and MMSE and BI scores in patients with AD was analyzed.Results The levels of CRP and IL-6 in the AD group were significantly higher than those in the control group,the difference was statistically significant(P<0.05);The scores of directional function,instantaneous memory,attention and computational power were significantly lower in the AD group than those in the control group,the difference was statistically significant(P<0.05);The daily living ability scores of patients in AD group were lower than those in the control group,such as eating,bathing and dressing,and the difference was statistically significant(P<0.05);Serum CRP and IL-6 levels in patients with AD were negative correlation with MMSE and BI scores(P<0.05).Conclusion The levels of CRP and IL-6 in serum of patients with AD are high,and are closely related to the patients′ mental symptoms and daily living ability.

On the anatomy, posterior vitreous cortexneighbors with retina, the relationship of vitreous and retinal is both independent and closely linked. Under pathological condition, changes in the vitreous provides a good environment for occurrence and development of a number of vitreoretinal diseases, which indicates that vitreous plays a crucial role in many vitreoretinal diseases occurrence and growth. Elimination of vitreous change in disease, is an issue of great concern of the ophthalmic industry in recent years. Based on the description of vitreous and vitreoretinal interface structure, changes in the vitreous and retinal adhesion mechanisms and interfaces retinal disease risk factors and the impact on the retinal disease were discussed, And the posterior vitreous detachment impact on the vitreoretinal interface disease, the testing methods and its importance were described in this article.

Objective To evaluate the effects of biopsy methods, biopsy timing and the number of cell removed on in vitro development of embryos Methods One hundred and fifty four embryos of good morphology from in vitro fertilization patients were studied Sixty six embryos were allocated to the following three groups: chemical drilling biopsy group (26), mechanical drilling biopsy group (26) and control group (20) One cell was removed from the embryos of the two biopsy groups The remaining 88 embryos were allocated to two groups: biopsy group (44) and control group (44) Two cells were removed from biopsy group by chemical drilling method The stage of the embryo before biopsy, biopsy time, lysed blastomere, growth potential and hatching capacity of the biopsied embryos, total cell number at the blastocyst stage were recorded and evaluated Results The mean time of biopsy in the chemical drilling group (231?20) seconds was significantly shorter than that in the mechanical drilling group (262?23) seconds ( P 0 05) However, the proportion developing to the blastocyst stage was reduced after the removal of two cells from the 6 cell stage in comparison to the control (1/8 versus 5/8, P

Mammalian target of rapamycin ( mTOR) is a kind of Ser/Thr kinase existing in mammalian cells can regulate cell proliferation, migration and angiogenesis.mTOR including two different complex forms as mTORC1 and mTORC2 .Rapamycin and rapalogs were soon found to have powerful immunosuppressant prop-erties and thus be valuable for preventing the progress of the artery atheromatous by inhibiting the function of mTORC1.But long-term rapalogs administration may increase the side actions such as mTORC1 resistance, mTORC2 inhibition, dyslipidemia.The clinical application of drug combination and dosage optimization may reduce adverse events.