OBJECTIVE:To establish the quality standard of Danchong liniment.METHODS:Cortex Moutan,Radix Isatidis,Rhizoma Paridis,Fructus Crataegi and Fructus Mume in the formula were identified qualitatively by TLC.The content of Paeonol was determined by HPLC in which Hypersil C18 column(150 mm?4.6 mm,5 ?m)was used with mobile phase consisted of methanol-water(45∶55)at a flow rate of 1 mL?min-1.The detection wavelength was set at 274 nm and the column temperature was set at 30 ℃.RESULTS:Clear characteristic spots of Cortex Moutan,Radix Isatidis,Rhizoma Paridis,Fructus Crataegi and Fructus Mume were noted in TLC identification.The linearity of Paeonol was good in the range of 0.048 2～0.482 0 ?g(r=0.999 9)with an average recovery of 99.01%(RSD=1.60%,n=6).CONCLUSION:The established quality standard can be used for the quality control of Danchong liniment.

Objective: To establish the quality control standard for Xiaozhi Solution(Radix et Rhizoma Rhei, Radix Scutellariae, Radix Paeoniae Rubra, Rhizoma Smilacis Glabrae, etc.) Methods: Radix et Rhizoma Rhei Radix Scutellariae、 and Radix Paeoniae Rubra in solution were identified by TLC. The contents of emodin chrysophanol were determined by HPLC. The average recovery of added sample were 99.10% ( RSD was 1.88% ) for emodin and 98.87% ( RSD was 1.21%) for chrysophanol. Results: The method was simple, accurate and specific. Conclusion: The method can be used for the quality control of Xiaozhi Solution.

This 9-year-old boy presented with dysphonia and dyspnea for one month. Laryngoscopy showed a tumor of anterior vocal cord. The patient received a tumorectomy, immunohistochemistry concluded to the diagnosis of inflammatory myofibroblastic tumor.
Child ; Humans ; Laryngeal Neoplasms ; Male ; Myofibroma

Objective To continually observe the monocyte chemoattraetant protein-1(MCP-1) expression in rats with anilateral ureteml obstruction, and to explore the relationship between MCP-1 and p38MAPK, and as well as the nuclear transcription faetor-кB (NF-кB). Method Thirty-six rots were randomly assigned to sham op-eration group (contorl group) and unilateral ureteral obstruction group (UUO group). Renal tissues were examined under light microscope 8 h,24 h and 72 h after operation, Immunhistochemistry was used to measure the expression of MCP-1 and NF-кB. RT-PCR and Western bot were employed to determine MCP-1 mRNA and p38MAPK pro-tein,respectively.Serum creatinine and ure nitrogen were examined with biochemistry methods. Results Com-pared with control group, the expression of MCP-1 mRNA in UUO group dramatically increased (control group:0.401±0.039;UUO group 8 h:0.894±0.137;24 h: 1.416±0.135;72 h: 1.894±0.14, P<0.05).The explosion of MCP-1 protein in renal interstitium of UUO group also markedly increased (control group: 50.08±3.210. UUO group 8 h:108.25±4.325;24 h: 179.34±3.237;72 h: 230.12±3.026, P<0.05),and the expressions of NF-кB and p38MAPK in UUO group were also stimulated (p38MAPK: control group: 110.65±9.734. UUO group 8 h:200.15±8.326; 24 h:272.74±7.244;72 h:549.11±9.544, P<0.05). There were positive correlations between MCP-1 and p38MAPK as well as NF-кB(r=0.74,r=0.81,P<0.01).Conclusions Tne increased expression of MCP-1 may participate in the injury mechanisms of renal tubulointesti-tinm in UUO rats. The increased expression of p38MAPK may induce NF-кB expression in the tubules, and then NF-кB promotes the expression of MCP-1.

Objective To evaluate the association between serum 25-hydroxyvitamin D3 [25 (OH)D3] and arterial stiffness in patients with chronic kidney disease (CKD).Methods Three hundred patients with CKD were included,and were divided into two groups based on serum 25(OH)D3 levels:vitamin D deficient [25(OH)D3 ＜ 20 μg/L] and vitamin D non-deficient [25(OH)D3≥20 μg/L].Brachial ankle pulse wave velocity (baPWV),which reflected arterial stiffness,was calculated using the single-point method.Clinical data were collected in details.Correlation between serum 25(OH)D3 level and baPWV was assessed by the single factor correlation test and multiple linear regression analysis.Results The prevalence of vitamin D deficiency was 62.7％(188/300).The concentration of 25(OH)D3 was (17.62±8.54) μg/L in total patients,but was (12.38±4.55) μg/L and (26.44±6.05) μg/L in the subgroups of vitamin D deficient and non-deficient,respectively(P ＜ 0.01).There was a higher value of baPWV in the group of vitamin D deficient than that of vitamin D non-deficient (1 827.34±429.11 vs 1 555.31 ±353.14,P ＜ 0.01).Serum 25(OH)D3 level and baPWV was negatively correlated in total patients(r=-0.38,P ＜ 0.01) and each stage of CKD(stage 2-5)[r=-0.30,P ＜ 0.05; r=-0.26,P ＜ 0.05; r=-0.46,P ＜ 0.01; r=-0.57,P ＜ 0.01].Multiple linear regression analysis showed that vitamin D level was independently associated with baPWV(Model 1:β=-0.18,P ＜ 0.01; Model 2:β=-0.17,P=0.01).Both models accounted for 50％ (R2=0.50) of total variance of baPWV.Conclusions Vitamin D deficiency is common in CKD,and a low 25(OH)D3 level is significantly associated with increased arterial stiffness in these patients.Clinical intervention studies are needed to clarify whether treatment with vitamin D decreases the risk of cardiovascular disease in patients with CKD.

Objective To characterize the CT features of peripheral small lung cancers of fast growthing and to correlate such features with pathological findings.Methods 12 peripheral small lung cancers(

Objective To investigate the changes of activation and apoptosis of T-lymphocyte in peripheral blood of patients with chronic obstructive pulmonary disease(COPD) at the attack and relieved stage and their clinical significance. Methods 64 cases of COPD were divided into 2 groups: the acute stage(n=34)and the relieved stage groups(n=30). The T-lymphocytes were marked by fluoromonoantibodies CD 3 +, CD 4 +, CD 25 + , CD 95 +, CD 3 +/CD 25 +, CD 8 +/CD 28 +,CD 8 +/CD 28 - and was counted by flow cytometry . Results Compared with the relieved stage, in patients with COPD at the acute attack period,the CD 3 +, CD 95 +, CD 3 +/ CD 25 +, CD 8 +/ CD 28 - were increased, CD 4 + ,CD 25 + had not obvious change, but CD 8 +/ CD 28 + was increased. Conclusions In COPD at the acute attack stage, the immune function was disordered as the result of the change of T subtype of lymphocytes and the reduction of total T lymphocyte.So except for treatment in accordance with their symptoms,immunodrug of cells should be used in order to raise the immune function of the patients.

Objective To investigate the combination of blepharoplasty with rhytidectomy for correction of the aging face. Methods In addition to conventional rhytidectomy, the operation included transection of lower orbicularis palpebrorum ligament ,excision of the herniated orbital fat, suspension of lower obicularis palpebuorum muscle. Results The method has produced a satisfied result in 25 cases since 1999. Conclusion On the base of conventional rhytidectomy, we transect the lower orbicularis palpebrorum ligament to elevate and stabilize the orbicularis muscle to the periosteum above the level of lateral canthus, which improves nasopalpebromalar groove and obtains more youthful appearance of the mid face.

AIM: To determine tanshinone Ⅱ_A and salvianolic acid B by HPLC gradient elution. METHODS: HPLC was used with Hypersil-ODS C_(18) column(150 mm?4.6 mm,5 ?m).The mobile phase consisted of methanol-water 0~20 min(35-90:6510),20-25 min(90-95:10-5),25-30 min(95:5)gradient elution.The flow rate was 1.0 mL/min with column temperature at 30 ℃.The UV detection wavelength was at 286 nm in 0-20 min and 270 nm in 20-30 min,respectively. RESULTS: The linear range of tanshinone Ⅱ_A and salvianolic acid B were in the range of 0.014 448-0.096 32(r=0.999 97), and 0.198 6-3.972 ?g(r=0.999 94),respectively.The average recoveries were 99.62%(RSD=2.28%) and 100.58%(RSD=2.5%),respectively.(CONCLUSION:) The method is accurate、sensitive、reproducible,and suitable for the quality control of Chaidan Jieyu Granules.

Objective To optimize the extract technology of Quyou Tincture with orthogonal design.Methods With the determination of peoniflorin and paeonol and the extraction rates as indexes,the extract conditions of Quyou Tincture was optimized by orthogonal design.Results The optimal preparation process of alcohol was as follows:adding 6 times alcohol(75%),immerse 24 hours and percolate with 1 mL/min.Conclusion The optimum extracting condition was simple,with a high extraction rate and low cost.