1.Application of nursing safety management system in operating room
Dan WU ; Qulian SHI ; Yaoqin LI ; Shunhua DENG ; Yuxia CHEN
Modern Clinical Nursing 2017;16(3):56-59
Objective To establish nursing safety management system in the operating room and explore the effect of clinical application.Methods Toally 350 patients with surgical treatment from January to December 2013 were assigned as the control group,where routine nursing safety management was conducted.Another 350 patients from June 2014 to June 2015 were as the observation group,where nursing safety management system was used in the operating room.The two groups were compared in terms of adverse events,the passing rate of quality and safety.Result As compared with the contrast group,the incidence of adverse events was significantly lower and the passing rate of quality and safety was significantly higher in the observation group (P<0.05).Conclusion Establishment of nursing safety management system can improve the nursing risk awareness,reduce the incidence of preoperative and postoperative adverse events and improve the level of nursing safety management and the quality of care.
2.Screening for urine biomarkers in steroid-resistant nephrotic syndrome using SELDI-TOF-MS
Mei YANG ; Wenjun LIU ; Qulian GUO ; Junhong CHEN ; Han SHI ; Meixian HUNAG
Journal of Clinical Pediatrics 2010;(2):183-186
Objective To screen for biomarkers in urine from patients with steroid-resistant nephrotic syndrome (SRNS) using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) Proteinchip technology. Methods Urine samples from 9 SRNS patients, 32 steroid-sensitive nephrotie syndrome (SSNS) patients and 45 normal controls were analyzed using UA gold chip. Proteomic spectra were generated by mass spectrometry. Results Four differentially expressed biomarkers were identified with relative molecular weight of 6 703, 7 212, 11 820, 14 356. It was found that these protein peaks with relative molecular weigh of 7 212, 11 820, 14 356 were highly expressed in SRNS and 6 703 were lowly expressed in SRNS. The diagnostic cast that is constructed with these four protein to differentiate SRNS from SSNS with sensitivity of 88.89% and specificity of 93.75%. Conclusions SELDI-TOF-MS Proteinchip technology is a non-invasive, quick, easy, and convenient, and high-throughput analyzing method capable of screening several biomarkers from the urines of SRNS patients and has better clinical value.
3.Sevoflurane preconditioning induced delayed neuroprotection against focal cerebral ischemia in rats.
Zhi YE ; Qulian GUO ; E WANG ; Min SHI ; Yundan PAN
Journal of Central South University(Medical Sciences) 2009;34(2):152-157
OBJECTIVE:
To investigate whether the reactive oxygen species (ROS) and mitochondrial ATP-sensitive potassium (mitoKATP) channels were involved in delayed neuroprotection induced by sevoflurane on tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) levels.
METHODS:
Eighty-four male SD rats weighing 250 approximately 280 g, undergoing thread embolism of the right middle cerebral artery occlusion (MCAO) to cause focal ischemia for 2 h and then undergoing 24 h reperfusion, were randomly divided into 7 groups (n=12, each): a sham group(S), an ischemia-reperfusion group (I/R), a sevoflurane preconditioning group (Sevo), a 2-mercaptopropionylglycine (ROS scavenger)+sevoflurane group (MPG+Sevo), a 5-hydroxydecanoate (a mitoK(ATP) blocker) + sevoflurane group (5-HD+Sevo), an MPG group, and a 5-HD group. The protein level of TNF-alpha and IL-1beta in the cerebral issue was detected by enzyme linked immunosorbent assay (ELISA) and the expression of mRNA was measured by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTS:
Apoptosis index (AI), the protein level and the mRNA expression of TNF-alpha and IL-1beta were significantly higher in the I/R group than those of Group S. Pre-administration of sevoflurane could inhibit the increase of the protein level and the expression of mRNA of TNF-alpha, and IL-1beta and attenuate the cerebral damage induced by ischemia-reperfusion. Neuroprotection of sevoflurane preconditioning was abolished by MPG and 5-HD. However, MPG and 5-HD alone had no effect.
CONCLUSION
Sevoflurane can produce delayed protection against cerebral ischemia-reperfusion injury by down-regulating TNF-alpha, IL-1beta protein, and mRNA expression.
Animals
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Hypoxia-Ischemia, Brain
;
complications
;
drug therapy
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Infarction, Middle Cerebral Artery
;
complications
;
drug therapy
;
Interleukin-1beta
;
genetics
;
metabolism
;
Ischemic Preconditioning
;
methods
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KATP Channels
;
metabolism
;
Male
;
Methyl Ethers
;
pharmacology
;
therapeutic use
;
Neuroprotective Agents
;
pharmacology
;
therapeutic use
;
RNA, Messenger
;
genetics
;
metabolism
;
Random Allocation
;
Rats
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Rats, Sprague-Dawley
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Reactive Oxygen Species
;
metabolism
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Reperfusion Injury
;
etiology
;
prevention & control
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Sevoflurane
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Tumor Necrosis Factor-alpha
;
genetics
;
metabolism