1.Comparative Proteome Analysis of Plasma Membrane from Different Differential Nasopharyngeal Carcinoma Cell Lines
Tingting SHENG ; Lijun ZHANG ; Xiaohui LIU ; Xiaofang JIA ; Jixian XIONG ; Quanyuan HE ; Rui CAO ; Xia PENG ; Ni SHI ; Songping LIANG
Chinese Journal of Biochemistry and Molecular Biology 2008;24(8):712-718
A subcellular proteomic method was applied to investigate the protein expression profiles of nasopharyngeal carcinoma (NPC) cell lines,CNE1 and CNE2,at various differentiation levels.Plasma membrane (PM) proteins were obtained by Percoll density grade centrifugation and subjected to twodimensional electrophoresis (2DE) followed by PDQuest software analysis.Nine proteins expressed with more than two folds difference were identified by MALDI-TOF-TOF,of which functions involved in cell differentiation,signal transduction,and metabolism.Half of these proteins,such as galectin-1 and annexin Ⅱ,were analyzed with real-time quantitative PCR or Westem blotting.We have tested a proteomic method to study differentiated NPCs at different levels and found several proteins that might be related to their biological characteristics.
2.Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation.
Guang SHI ; Yaofu BAI ; Xiya ZHANG ; Junfeng SU ; Junjie PANG ; Quanyuan HE ; Pengguihang ZENG ; Junjun DING ; Yuanyan XIONG ; Jingran ZHANG ; Jingwen WANG ; Dan LIU ; Wenbin MA ; Junjiu HUANG ; Zhou SONGYANG
Protein & Cell 2022;13(10):721-741
Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro. In this study, we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation (BiFC) platform for protein-protein interaction screens and epiblast-like cell (EpiLC)-induction assays using reporter mouse embryonic stem cells (mESCs). Investigation of candidate interaction partners of core human pluripotent factors OCT4, NANOG, KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell (PGC)-inducing factors including BEN-domain (BEND/Bend) family members. Through RNA-seq, ChIP-seq, and ATAC-seq analyses, we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro. Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development.
Animals
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Cell Differentiation/genetics*
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Chromatin/metabolism*
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Embryonic Stem Cells
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Germ Cells/metabolism*
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Germ Layers/metabolism*
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Mice
Result Analysis
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