Aim To study the protective effects of bFGF on rats’ renal tubular epithelial cells injured by gentamicin in vitro.Methods After renal cortexes were grinded,filtered and digested with 0.25% trypsin,renal tubular epithelial cells were cultured.The cell types of epithelial cells were identified by cytokeratin 18 immunocytochemistry.The injury models of epithelial cells of renal tubules were established by gentamicin and then the protective roles of bFGF on injured renal tubular epithelial cells were observed. Results ①By observing the morphology of the epithelial cells and identifying the cell types with cytokeratin 18 antibody,comparing GM group with control group,CAT,Na+,K+-ATP enzyme activity decreased,while NAG activity and MDA level increased(P0.05),but the differences of MDA was still significant(P

Objective To observe the morphologic alterations of retina pigment epithelial around fluorescein leakage site using spectral-domain optical coherence tomography (SD-OCT) in acute central serous chorioretinopathy (CSC) before and after laser coagulation.Methods Twenty-four eyes of 24 consecutive patients with unilateral acute CSC who underwent laser photocoagulation (wave length:561 nm,energy:80-100 mW,spot diamteter:100 μm,exposure time:0.1 s) were enrolled in this retrospective study.The patients included 22 males and 2 females,with an average age of (39.2± 7.2) years and an average duration of (1.1 ± 0.6) months.The fluorescein leakage outside the foveal avascular zone and serous retinal detachment were found in all the eyes by fluorescein fundus angiography (FFA) and SD-OCT.Twenty-six leakage sites were observed in 24 eyes on FFA.There were 5 smoky leakage points (19.2％),21 inky leakage points (80.8 ％).The retinal pigment epithelial (RPE) layer at leakage points were scanned by SD-OCT before and at 2 weeks,2 months after treatment.SD-OCT showed that the RPE abnormalities were detected at all of the fluorescein leakage points,including serous pigment epithelial detachment (PED) in 16 leakage points (64.5％),protruding RPE in 5 leakage points (19.5％),and irregular RPE in 5 leakage points (19.2％).The morphologic alterations of RPE around fluorescein leakage point before and after laser coagulation were observed.Results At 2 weeks after laser photocoagulation,PED existed in 7 leakage points (26.9％),protruding RPE in 3 leakage points (11.5％),and irregular RPE in 16 leakage points (61.5％).At 2 months after laser photocoagulation,protruding RPE existed in 3 leakage points (11.5％),and irregular RPE in 5 leakage points (19.2％),including RPE defect in 2 leakage points.And there were undetectable RPE abnormalities in other 16 leakage points (61.5％).Conclusions In acute CSC,SD-OCT shows that morphologic features of the RPE layer are changed,including PED,protruding RPE,irregular RPE around the leakage point.After laser photocoagulation the abnormalities of RPE can also be altered from PED to irregular RPE,and RPE defect is observed in restricted regions.

Objective: To investigate the interaction between traditional Chinese medicine (TCM) syndromes of histiocytic necrotizing lymphadenitis (HNL) and pathological types of HNL, so as to discover the distribution rule of TCM syndromes for indiction of intervention of HNL. Methods: A total of 166 patients with HNL were included. The baseline data of the patients, clinical feature, the four diagnosis information of TCM and histopathological of cervical lymph node were collected. The syndromes of TCM were judged. The pathological types were confirmed by cervical lymph node biopsy. The Multi-way ANOVA was used to analyze the correlation between traditional Chinese medicine syndromes of HNL and its pathological types. Results: Among the 166 patients, the ratio of different types are as follow: wind-heat and phlegm-toxin syndrome (57.83%, 96/166), heat-toxin exuberance syndrome (34.34%, 57/166), and phlegm and blood stasis syndrome (7.83%, 13/166). The pathological types include proliferative type (57.83%, 96/166), necrotizing type (35.54%, 59/166), and xanthomatous type (6.63%, 11/166). There were significant differences in targeted lymph node diameter, pain scores, body temperature (F value were 3.737, 34.484, 34.805, all Ps<0.05), while the difference of WBC (F=1.194, P>0.05) among three TCM Syndrome types were notsignificant. There were significant differences in pain scores, body temperature (F value were 12.153, 25.931, all Ps<0.05), but not for the targeted lymph node diameter, WBC (F value were 2.249, 0.671, all Ps>0.05) among three pathological types. The corresponding analysis result showed that, on point sets, wind-heat and phlegm-toxin syndrome were correlated with proliferative type, heat-toxin exuberance syndrome correlated with necrotizing type, and phlegm and blood stasis syndrome correlated with xanthomatous type (χ²=235.100, P<0.001). Conclusions There is a close correlation between syndrome types of TCM and pathological types of HNL. The pathological change of wind-heat and phlegm-toxin syndrometends toproliferative type, heat-toxin exuberance syndrome tends to necrotizing type, and phlegm and blood stasis syndrome tends to xanthomatous type.

¹⁷⁷Lu- prostate specific membrane antigen (PSMA) radio-ligand therapy has been approved abroad for advanced prostate cancer and has been in several clinical trials in China. Based on domestic clinical practice and experimental data and referred to international experience and viewpoints, the expert group forms a consensus on the clinical application of ¹⁷⁷Lu-PSMA radio-ligand therapy in prostate cancer to guide clinical practice.