OBJECTIVE:To evaluate the drug utilization in nationwide sample hospitals so as to provide information for the production, management and utilization of drugs. METHODS: The drug utilization data in nationwide 690 hospitals during the period 2005 ～2006 were analyzed statistically. RESULTS & CONCLUSION: Anti-infective drugs, cardiovascular drugs, biological products and biochemical drugs have been ranking among the first 3 places in consumption, but their consumption sum showed a downward trend. The consumption sum of anti-tumor drugs, Chinese formulated products, digestive system drugs, and respiratory system drugs showed an upward trend. The top 20 drugs on the list of consumption sum were mainly antibiotics. The consumption quantity and consumption sum of anti-tumor drugs showed an upward trend. Of the top 10 manufacturers in terms of consumption sum, the imported manufacturers took a small proportion while the joint venture took a higher proportion.

Objective To discover the knowledge level about the COPD of doctors in District and Community Hospitals. Methods The knowledge level of COPD was investigated with questionnaire in 400 physicians in 10 districts of Beijing in December 2007. Content included: COPD definition, diagnosis, epidemiology, risk factor, COPD common symptoms, exacerbation manifestation, COPD non-pharmacological treatment (pulmonary rehabilitation, smoking quitting, influenza and pneumococcal vaccination), and COPD pharmacological treatment (bronchodilator therapy, antibiotic therapy, inhaled and systemic corticosteroids therapy). Results There were 372 answered copies of questionnaire collected, the total average score was 50.1?20.6 points, pass rate was 42.5%, while was 55.2?20.6 points and 57.6% in 30~year old group and 55.6?14.2 points & 51.9% in 40~year old group, which were higher than those in 20~year old group and ≥50 year old group (P

ObjectiveTo understand knowledge level about chronic obstructive pulmonary disease (COPD)of medical doctors practising at district hospitals and community health service and impact of different models of continuing medical education on their knowledge level.MethodsKnowledge of COPD was investigated with a close-book examination in 372 medical doctors working at the grassroots of 10 urban copies of finished examination papers were returned in this investigation.with an average score of 50.1 and pass rate of 42.5%.About 67.7%and 67.5%of medical doctors passed the examination of knowledge on definition,risk factors and non.pharmacological treatment for COPD.respectively,and 19.9%of them passed the examination on pharmacological treatment for COPD.Different modes of continuing medical education made varied impacts on their acquisition of COPD knowledge.with an average score of 67 and pass rate of 73.3%in the intensified education group,respectively,significantly higher than those in general education group and non-education group.ConclusionsClinical knowledge and skills of COPD for medical doctors working at the grassroots in Beijing Was not so satisfactory and education should be strengthened urgently to raise their knowledge on COPD prevention and control.

OBJECTIVE:To analyze the status quo and tendency of TCM injection in our hospital. METHODS:Consumption sum,DDDs and DDDC of TCM injections in our hospital from 2006 to 2009 were analyzed statistically. RESULTS:The consumption sum of TCM injections increased year by year. The consumption sum of TCM injection was increased by 40.24% in 2007 and 32.66% in 2008 while it was decreased by 1.84% in 2009. The proportions of consumption sum of TCM injection in total consumption sum of drugs in 4 years were 2.59%,3.43%,3.98% and 3.30% respectively,showing yearly increasing trend. CONCLUSION:The application of TCM injection in our hospital shows a reasonable increase. Clinical pharmacists have rational reaction to ADR and adverse events caused by TCM injections.

Objective To establish a liquid chromatography-tandem mass spectrometric ( LC-MS/MS ) for determination of clopidogrel in human plasma and stability of clopidogrel under different conditions,which was used subsequently to investigate the pharmacokinetics of clopidogrel in healthy Chinese volunteers. Methods Clopidogrel-d4 hydrogen sulfate was used as an internal standard. Separation was achieved on a WATERS Xterra? RP18 column (4.6 mm× 100 mm,3.5μm) with a mobile phase consisting of acetonitrile-0.1% formic acid (66:34) at a flow rate of 1.0 mL.min-1 within 3.2 min. ESI source was applied and operated in positive ion mode and multiple reaction monitoring (MRM). Plasma samples were pretreated by acetonitrile precipitation. Results A good linearity of clopidogrel was obtained in the concentration range of ( 5-5 000 ) ng.L-1. The lower limit of quantification was 5 ng.L-1. The intra-and inter-run precisions at three quality control levels were within 1.3%–9.9%,the relative deviation of the assay was within -6.2%-14.3%. The blood samples were stable when chilled with crushed ice ( 0℃) and cold water ( 4℃) ,as well as kept at room temperature ( 20℃) for 40 min. The plasma QC samples were stable at room temperature ( 20 ℃) for 4 h,at -70℃ for 38 days and during three freeze-thaw cycles. Hemolysis in blood sample drawn didn’ t affect the plasma concentration. The determination and the result of incurred sample reanalysis met the requirements. Conclusion A specific, rapid, sensitive and stable LC-MS/MS method is developed and validated for determination of clopidogrel in human plasma. The method is proven to be suitable for study of the pharmacokinetics of clopidogrel in healthy Chinese volunteers after a single oral dose of 75 mg clopidogrel hydrogen sulphate tablet.

This paper introduces the concensus on the relationship between sleep apnea and cardiovascular disease reached by experts with corresponding backgrounds from Chinese Medical Association.The relationship is that of sleep apnea with hypertension,coronary artery disease,arrhythmias,and congestive heart failure,and the relationship can improve the diagnosis and treatment of these two types of diseases.

Objective To investigate underdiagnosis problem of chronic obstructive pulmonary disease (COPD) in China.Methods Articles published during January 1 st,2000 and December 30th,2011were searched in Wanfang Database and Medline,search words including COPD and epidemiology survey.The papers were then reviewed,and those original contirbutions with sample size ≥ 1000 and strict quality control entered into the final analysis.Results Only 32.90％ (1095/3328) COPD patients had ever been diagnosed to have emphysema,bronchitis or COPD,and only 9.13％ (237/2597) had undergone lung function test.About 65.40％ (2306/3526) COPD patients were presented with at least one of the following symptoms:cough,phlegm and breathlessness.Stage Ⅰ or Ⅱ COPD was found in 74.52％ (1802/2418)patients.Conclusion Underdiagnosis of COPD was quite common in China,and patients with stage Ⅰ or Ⅱ COPD should have deserved early diagosis.

Objective To establish an LC-MS/MS method for the detection of landiolol concentration in human blood.Methods After pretreatment with neostigmine and a deproteinization procedure, landiolol and the internal standard venlafaxine were eluted isocratically using a mobile phase consisting of acetonitrile and 10 mmoL·L-1 ammonium acetate with 0. 1% formic acid in a ratio of 3664 ( V/V ) . Separation of the respective compounds was achieved on a Waters XTerra? RP18 column (150 mmí4. 6 mm,5 μm). Quantitative analysis of landiolol was conducted by a triple-quadrupole mass spectrometer with positive-electrospray ionization source,monitored under a multiple reaction monitoring ( MRM) mode. The extracted ions monitored following MRM transitions were m/z 510. 5→423. 1 for landiolol and m/z 278. 2→215. 1 for the internal standard venlafaxine. ResultsThe calibration curve of landiolol in human blood showed good linear relationship in the range of 1. 010-2 020 μg·L-1 . The lower limit of quantitation was 1. 010 μg · L-1 . The RSD of within-day and between-day precision was less than 6. 5% and 4. 8%, respectively. The recovery rate was 92. 6%-100. 9%. Conclusion The method is proven to be simple,rapid and reliable,and can be applied to study the pharmacokinetics of landiolol hydrochloride in healthy Chinese volunteers.

Objective: To study the effect of Interleukin(IL)-17 on neutrophil apoptosis and try to explain the possible mechanism involved. Methods: Neutrophils isolated from healthy donors were incubated in enriched RPMI 1640 cell culture medium at 37 ℃ in 5% carbon dioxide. Subgroups were incubated with IL-17, heat-denatured IL-17 (X-IL-17), dexamethasone (DEX), or buffer alone. Apoptosis was assessed by morphologic changes, by detecting DNA strand breaks. Production of proapoptotic protein Bax by neutrophils was evaluated by immunocytochemistry. Results: At the time of neutrophil incubation, neutrophils in the control subsets exhibited morphologic evidence of apoptosis. A steady rise in apoptosis index (AI) was noted, with (1.54?0.08)% for 0 h, (11.48?1.80)% (compared with 0 h, P0.05) for 0 h, (20.47?6.22)% ( compared with control 12 h, P0.05).Neutrophils apoptosis was accompanied by DNA fragmentation. In all groups, the increasing of Bax immunoreactivity was strongly related with more apoptotic neutrophils (r=0.932, P

A sensitive method based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous determination of folic acid (FA) and its active metabolite, 5-methyltetrahydrofolic acid (5-M-THF), in human plasma. The analytes were extracted from plasma with methanol solution containing 10 mg/mL of 2-mercaptoethanol and 0.025% (v/v) ammonium hydroxide. FA and 5-M-THF were more stable after the addition of 2-mercaptoethanol and ammonium hydroxide in the sample preparation procedures of this study than they were in the previously published methods. Chromatographic separation was performed on a Hedera ODS-2 column using a gradient elution system of acetonitrile and 1 mM ammonium acetate buffer solution containing 0.6% formic acid as mobile phase. LC-MS/MS was carried out with an ESI ion-source and operated in the multiple reaction monitoring (MRM) mode. The assay was linear over the concentration ranges of 0.249-19.9 ng/mL for FA, and 5.05-50.5 ng/mL for 5-M-THF. The developed LC-MS/MS method offers increased sensitivity for quantification of FA and 5-M-THF in human plasma and was applicable to a pharmacokinetic study of FA and 5-M-THF.