Objective To investigate the analgesic and anti-inflammatory effects of diuretic mixture and its bacteriostasis effect in vitro,and to provide scientific basis for clinical application.Methods Ear swelling test induced by xylene,twisting reaction test induced by acetic acid,capillary permeability increase in abdominal cavity of mice induced by acetic acid,and pain test induced by formalin were used to observe the analgesic and anti-inflammatory effects of diuretic mixture at high,middle and low doses (crude drug 18.0,9.0,and 4.5 g/kg).Bacteriostatic activities of diuretic mixture were tested by K-B paper disc diffusion method.Results Diuretic mixture alleviated ear edema in mouse model at high dose (P ＜ 0.01).Diuretic mixture at high,middle,and low dose could effectively decrease the twisting reaction (P ＜ 0.01),inhibit capillary permeability (P ＜ 0.05 or 0.01),and ease the ache degree of mice induced by formalin in the first phase (P ＜ 0.01),but there was no significant difference in the degree of pain intensity of phase Ⅱ.The inhibitory rates of diuretic mixture on Staphylococcus aureus and Escherichia coli were 95.04％ and 37.44％,respectively.Conclusion Diuretic mixture has significant effects on analgesia and anti-inflammation and against S.aureus and E.coli in vitro.

Ninety six female patients with chronic renal failure were randomly allocated into combination group (n =48) and control group (n =48).In combination group patients received both kidney transplantation and hematopoietic stem cell infusion,in control group patients underwent kidney transplantation only.The results showed that chronic rejection in the combination group was lower than that in the control group [2％(1/48)vs.17％ (8/48),P＜0.05)].The 1-,3-,5-and 10 y-survival rates of kidney in the combination group were 98％ (47/48),94％ (45/48),83％ (34/41) and 9/17,respectively,those in control group were 98％ (47/48),90％ (43/48),76％ (31/41) and 7/17,respectively.Infusion of donor hematopoietic stem cells can augment chimerism in early postoperative period and significantly reduce the rate of graft rejection,which is beneficial for the quality of life of the recipients.