Objective To comparatively study the advantage and disadvantage of different polysaccharide extraction methods of enzymatic extraction,ultrasonic extraction and a combination of these two processes.Methods The enzymatic extraction and ultrasonic extraction were first optimized by orthogonal design and then compared with the effect of combination.Results From the results of three extraction techniques,enzymatic extraction of Talinum crassifolium polysaccharide's average extraction rate was 17.45%,ultrasonic extraction was 20.01%,enzyme-ultrasonic extraction was 20.74%.Conclusion The combined optimum preparative procedure of Talinum crassifolium is simple,quickly and high efficient.