1.Expansion Design and Experimental Study on Knowledge Base of the Therapeutic Model for Treatment with Prescriptions of Traditional Chinese Medicine
Fan ZHANG ; Tingge REN ; Quanquan GAO ; Xiaofeng LIU ; Yan SUN ; Yongyi CHEN ; Pengna ZHAO
Chinese Journal of Information on Traditional Chinese Medicine 2014;(9):13-16
Objective To perfect the prescription knowledge discovery methods; To discover the key factors affecting the robustness of prescription therapeutic model as well as improve its recognition capability.Methods Expanded knowledge base and improved design of Chinese Medicine Prescriptions Intelligence Analytic System (CPIAS) were proposed, such as the establishment of the heuristic filtering rules of efficacy-syndrome relationship, knowledge table of efficacy-syndrome element relationship, identification of efficacy-syndrome element relationship, and syndrome element-syndrome relationship. In addition, quantitative data were calculated by CPIAS. Prescription therapeutic modeling experiments on the Chinese medicine prescriptions system were conducted based on support vector machine (CPSVM), which was also used to analyze the learning outcomes.Results Using expanded knowledge base and improved calculation results can significantly promote learning abilities of CPSVM.Conclusion Screening of efficacies, sorting of symptoms, and collection of syndrome elements are the key factors affecting the quality of prescription therapeutic model.
2.Establishment of human gastric cancer model in normal immune mice based on micro-carrier 6
Yanzhen BI ; Lingbin KONG ; Pengfei GAO ; Quanyi WANG ; Yonghong YANG ; Xiaobei ZHANG ; Zeng FAN ; Quanquan WANG ; Bingcheng HUANG ; Feng YANG ; Qiusheng ZHANG ; Yibo WANG ; Fuqiang SUN ; Ye HONG ; Feng HONG
Chinese Journal of Clinical Oncology 2017;44(5):199-203
Objective:To establish a mouse model of gastric cancer by inoculating MKN45 cells into mice with normal immune function utilizing microcarrier technology. Methods:A total of 60 male C57BL/6 mice were randomly divided into three groups, namely, 2D, con-trol, and 3D groups, according to the coculture system of MKN45 and microcarrier. The mouse models of gastric carcinoma were estab-lished by hypodermic injection. The time of tumorigenesis, rate of tumor formation, and pathological features were observed in each group. Results:In the 3D group, the time of tumor formation was short, whereas the rate of tumor formation was high (80%). No de-tectable tumor formations were observed in the 2D and control groups. HE and immunohistochemical staining of the transplantation tumor model showed evident characteristics of human gastric cancer. Conclusion:A human gastric cancer model in normal immune mice was successfully established. The onset and development mechanism of gastric cancer could be more effectively investigated in mice with normal immune function through this model. Moreover, a more valuable and new animal model for the research and devel-opment of anticancer drug was established.
3. Protective effect of intraperitoneal transplantation of human liver-derived stem cells at different times against concanavalin A-induced acute liver injury in mice
Yanzhen BI ; Zeng FAN ; Dongfeng CHEN ; Shanshan LI ; Quanyi WANG ; Pengfei GAO ; Quanquan WANG ; Zhongping DUAN ; Yu CHEN ; Lingbin KONG ; Yibo WANG ; Feng HONG
Chinese Journal of Hepatology 2017;25(3):205-210
Objective:
To investigate the protective effect of intraperitoneal transplantation of human liver-derived stem cells at different times against concanavalin A (ConA)-induced acute liver injury in mice.
Methods:
A total of 88 male C57BL/6 mice were randomly divided into normal control group (group C), ConA model group (group M), and human liver-derived stem cells (HYX1)+ConA group (group E); according to the interval between phosphate buffer/HYX1 injection and ConA injection, Groups M and E were further divided into 3-hour groups (M1 and E1 groups), 6-hour groups (M2 and E2 groups), 12-hour groups (M3 and E3 groups), 24-hour groups (M4 and E4 groups), and 48-hour groups (M5 and E5 groups). The levels of alanine aminotransferase (ALT), aspartate transaminase (AST), and total bilirubin (TBil) in peripheral blood were measured, liver tissue sections were used to observe pathological changes, and the Ishak score for liver inflammation was determined. The independent samples t-test was used for comparison between groups, and