1.Determination of Chlorogenic Acid in Pneumonia Mixture by RP-HPLC
China Pharmacy 1991;0(03):-
OBJECTIVE:To establish a RP-HPLC method for the determination of Chlorogenic acid in Pneumonia mixture. METHODS: The separation of samples was performed on a column of Spheri-5 RP-C18 (250 mm?4.6 mm,5 ?m). The mobile phase consisted of acetonitrile-0.4% phosphate (8.5∶91.5) with a flow rate of 1.0 mL?min-1.The detection wavelength was set at 328 nm. RESULTS: The calibration curve was linear in the range of 5.00~100.00 ?g?mL-1 for Chlorogenic acid(r=0.999 9).The average recovery was 98.15%,(RSD=3.68%,n=6).CONCLUSION: The method was rapid, simple and accurate, and it can be used for the quality control of Pneumonia mixture.
2.Content Determination of Chloramphenicol and Salicylic Acid in Acne Tincture by HPLC
China Pharmacy 2015;(18):2557-2558,2559
OBJECTIVE:To establish a method for the content determination of chloramphenicol and salicylic acid in Acne tinc-ture. METHODS:HPLC method was adopted with the column of Shim-pack CLC-ODS with the mobile phase of methanol-water (50∶50,V/V)at the flow rate of 0.8 ml/min. The detection wavelength was 278 nm,column temperature was 35 ℃,and the vol-ume was 10 μl. RESULTS:The linear range was 2.0-10.0 μg/ml(r=0.999 2)for chloramphenicol and 5.0-25.0 μg/ml(r=0.999 1) for salicylic acid;the RSDs of precision,stability and repeatability tests were lower than 1%;the average recoveries chlorampheni-col and salicylic acid were respectively 99.77%(RSD=0.35%,n=9) and 99.29%(RSD=0.48%,n=9). CONCLUSIONS:The method is simple,rapid,accurate,specific,precise,and can be used for quality control of Acne tincture.
3.Analysis of Urine Arsenic Metabolites of People with Skin Lesion Caused by High Arsenide Exposure
Qiang ZHANG ; Quanmei ZHENG ; Shuhua XI
Journal of Environment and Health 2007;0(12):-
0.05), iAs% was much higher and the levels of FMR, SMR and DMA% were significantly lower in skin lesion group compared with the control (P
4.Effect of S100A8/A9 Protein Complex on F-actin Network in Human Cervical Carcinoma Cell Line,CasKi Cells
Xitao WANG ; Quanmei SUN ; Youyi ZHANG
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To investigate the effect of S100A8/A9 protein complex on the surface morphology and the F-actin network in human cervical carcinoma cell line,CasKi cells.Methods After being cultured with 20 ?g/ml S100A8/A9 protein complex,the cell skeleton of the CasKi cells were observed under a confocal scanning fluorescence microscope by staining the F-actin network.Atomic force microscopy(AFM) was employed to reveal the change of ultrastructure of the cell surface in vivo.ResultsAfter being cultured with the S100A8/A9 protein complex for 24 hours,the F-actin network disorder was revealed.Most of the F-actins distributed peripherally.The OD value of the F-actin decreased significantly from 92.42?5.16 to 57.67?3.70 after been treated with the S100A8/A9(t=5.268,P=0.000).The AFM showed a withdrawing morphology with reduced pseudopodia and destruction of stress fibers. Conclusion S100A8/A9 protein complex can change the ultrastructure of the surface of CasKi cells and its stress fibers by re-distributing of the F-actin in the cells.
5.Preparation and Quality Control of Qindiyou Emulsion
Quanmei ZHANG ; Jihong GE ; Maohui ZHANG ; Bing LIU
China Pharmacy 2005;0(22):-
OBJECTIVE:To prepare qingdiyou emulsion and to establish its quality control.METHODS:Qindiyou emulsion was prepared by emulsification by using tetracaine hydrochloride as principal agent.The content of tetracaine hydrochloride was determined by UV spectrophotometry.The stability of 3 batches of samples was investigated.RESULTS:The preparation was white emulsion.The linear range of tetracaine hydrochloride was 3.168~9.504 ?g?mL-1(r=0.999 9),with an average recovery rate of 99.68%(RSD=0.49%).The 3 batches of sample preparations were proved to be stable in quality.CONCLUSION:This method is simple in operation,accurate in content determination,and stable and controllable in quality within expiration date.