Objective To study the anti-dermatophytic effect of chitosan-acetate solution and to determine the antifungal MIC. Methods To determine the antifungal MIC of chitosan and acetic acid by agar dilution method,respectively. Results Both antifungi MIC of chitosan to Trichophyton rubrum and Trichophyton tonsurans were 2500mg?L~ -1 ,and to Trichophyton mentagrophytes,Microsporum gypseum,C. albicans were 5000mg?L~ -1;while the antifungi MIC of acetic acid to Trichophyton rubrum and Trichophyton tonsurans were 630mg?L~ -1, to Trichophyton mentagrophytes,Microsporum gypseum,Candida albicans were 1260mg?L~ -1. Conclusion Chitosan and acetic acid show inhibitory effect on the growth of dermatophytes.