Objective: To study the expression and significance of apoptosis related gene survivin in meningiomas.Methods: The expression of survivin protein was detected by immunohistochemical method(SP) in 60 cases of meningiomas,and its relationship with pathological parameters was analyzed.Results: The expression rate of survivin protein in meningioma of Ⅰ,Ⅱand Ⅲ grade were 32.0%,60.0% and 86.7% respectively,and there were great differences among the three groups(P

Objective:To investigate the level of excitatory amino acids in the cerebrospinal fluid and the effect of magnesium sulfate on the excitatory amino acids following traumatic brain injury. Methods:The Feeney′s model of the excitatory amino acids in the cerebrospinal fluid (glutamate and aspartate) were measured by amino acid analysis from rats in different time. Results:1. The glutamate and aspartate in the cerebrospinal fluid increased largely 2 hours after traumatic brain injury(P0.05).2.Magnesium inhibited strongly the releasing of the excitatory amino acids at 2h and 8h after the traumatic brain injury(P0.05).Conclusion:Plenty releasing of excitatory amino acid may play an important role at the phase of posttraumatic neuronal injury.Magnesium sulfate can inhibit early releasing of excitatory amino acids.Administering magnesium sulfate may be the optimal choice for treating traumatic brain injury.

Objective:To investigate apoptosis of neurone in rats following traumatic brain injury and the effects of magnesium sulfate on apoptosis of neurone after brain injury.Methods:Based upon the Feeney′s model the cellular apoptosis was observed in injuried cortex and hippocampus at the 2nd hour to the 5th day after medium traumatic brain injury by TUNEL technique.Results:1.The cellular apoptosis was observed in the injuried cerebral hemisphere at the 2nd hour after traumatic brain injury.The number of apoptotic cells reached its peak at the 2nd to the 3rd day after traumatic brain injury, then gradually reduced at the 5th day after traumatic brain injury.2.At the 8th hour to the 3rd day after treatment with magnesium sulfate,the number of the cellular apoptosis in the injuried cortex and hippocampus reduced significantly compared with the controls (P

Objective:To study the expression of apoptosis related gene survivin and its relationship with the expression of bcl-2,bax protein in glioma.Methods:Expression of survivin,bcl-2 and bax protein was detected by immunohistochemical method(SP) in 40 cases of glioma,and compared with those in 10 cases of normal brain tissue.The correlation between them was analyzed.Results:The expression rate(42.5%) of survivin protein in glioma was much higher than that(0%) in normal brain tissue(P

On the basis of my experiences in advanced neurosurgeons teaching,I realized that it was important to accomplish the following tasks:to make reasonable plan in advanced teaching,to constitute strict rules,to attach importance to clinical neuroanato- my teaching,to cultivate clinical ability,and to strengthen medical morality construction.Only by doing these can we improve the advanced education quality.

On the basis of experiences in guiding five-year system undergraduates in their clinic practice,we realized that it was important to accomplish the following tasks:both teachers and students should have good preparation for it;teachers should instruct students in interrogation and physical examination;teachers should cultivate students' clinic thoughts;teachers should help students to summarize their practice.

Great difficulties and challenges are existed in the training of clinical type postgraduate of neurosurgery because of the acute onset,server illness and difficult diagnosis of neurosurgical diseases.This paper proposed countermeasures of combing theory with clinical practice and strengthening comprehensive ability after analyzing problems of inadequate professional knowledge,lack of doctor-patient communication and insufficient clinical thinking and innovating ability.The aim was to cultivate the overall quality of postgraduates,making them more qualified for the difficult task of being neurosurgery clinicians.

Because of the specificity and complexity,of obstetrics and gynecology,interns often meet doctor-patient communication problems during the internship.This study explores these problems to enhance interns' doctor-patient communication and to cultivate interns' technical proficiency.We have also made them grasp the methods and skills of doctor-patient communication,and enable them to successfully communicate with patients on internship.

Clinical medicine professional degree education focuses on cultivating students' ability to engage in clinical practice, but the aim of medical postgraduate education program is to cultivate high-leveled clinical and research personnel. In the light of the problems that professional degree postgraduates majoring in obstetrics and gynecology are faced with such as unreasonable energy distribution in scientific research, the weak foundation of scientific research, the emergence of new trend of obstetrical and gynecological dis-eases, we have explored how to improve obstetrics and gynecology professional students' scientific research ability from several aspects such as the guidance of mentors, transferring learning and thinking and strength-ening the communication between various disciplines, to adapt to the requirements of the scientific develop-ment of modern obstetrics and gynecology.

[Objective]To forecast the sponge mechanism mediated by LOC389023 in patients with intractable temporal lobe epilepsy(TLE),through investigating the expression of microRNA interacted with dipeptidyl peptidase 10(DPP10)and LOC389023.[Methods]The expression of DPP10 and Kv4.3 were detected in 15 temporal neocortex from patients with brain trauma (control group)and in 26 temporal neocortex from patients with intractable TLE(epilepsy group)by western blot(WB)and immunohisto?chemical(IHC)staining. The location of DPP10 and voltage dependent potassium channel 4.3(Kv4.3)was detected by immunofluo?rescent(IF)staining. The interaction between DPP10 and Kv4.3 was testified by co-immunoprecipitation(Co-IP). The expression of microRNA obtained by softwares(miRanda,Pita,TargetScan and miRDB)was detected by qPCR.[Results]IHC and WB showed an increased expression of DPP10(P<0.05)and a decreased expression of Kv4.3(P<0.05)in the epilepsy group. IF showed that the DPP10 and the Kv4.3 co-expressed in the membrane and the cytoplasm of neurons. Co-IP showed obvious interaction between the DPP10 and the Kv4.3.Five microRNA(miR-32-5p,miR-140-5p,miR-367-3p,miR-25-3p,miR-4325)were obtained by soft?wares. No significant differences in the expression of miR-32-5p and miR-4325 were found between epilepsy group and control group by qPCR(P>0.05). But decreased expression of LOC389023 and miR-140-5p and increased expression of miR-25-3p and miR-367-3p were found in epilepsygroup compared to control group (P < 0.05).[Conclusion]miR-25-3p and miR-367-3p may be regulated by LOC389023 through the sponge mechanism followed by altered expression of DPP10 in intractable temporal lobe epilepsy.