Aim To investigate the expression and regulation characteristics of the Substance P receptor(Neurokinin-1R, NK-1R) in human skin keratinocytes and fibroblasts. Methods HaCaT cells,a human keratinocyte cell line, and fibroblasts were cultured. The expression of NK-1R protein was examined by immunohistochemisury technique,and the mRNA level was detected by reverse transcriptase polymerase chain reaction (RT- PCR). The expression and regulation of NK-1R were measured by flow cytometry in HaCaT cells and fibroblasts treated with various stimuli and drugs. Results The expression of NK-1R existed in human keratinocyte and fibroblast, mainly located on cell membranes and cytoplasma. The NK-1R also expressed at HaCaT cells and fibroblasts transcription levels, and the mRNA levels in HaCaT cells were higher than that of fibroblasts. SP and IFN-? might upregulate the membrane expressions of NK-1R in both the two cells, while LPS might downregulate the expressions of NK-1R. Cetirizine and Spantide I can degrade the expressions of NK-1R in the two kinds of cells.Conclusions The human keratinocytes and fibroblasts can express NK- 1R at cell, protein and transcription levels, and the expression characteristics can be regulated by some inflammatory factors, it indicates the keratinocytes and fibroblasts were involved in the regulation of skin immune and NK-1R may play an important role in skin inflammation.

Objective: To establish the quality standard for Fufang Dusheng ointments. Methods: Angelicae sinensis radix and Cinnamomi ramulus in Fufang Dusheng ointments were identified by TLC. Cinnamaldehyde was determined by HPLC. Results: The characteristic identification by TLC was distinct and highly specific. Cinnamaldehyde had the linear range of 5. 025-50. 255 μg·ml-1 (r=0. 999 9), and the average recovery was 98. 92% (RSD=1. 64%, n=9). Conclusion: The method is reliable, accurate and specific. It can be used in the quality control of Fufang Dusheng ointments.

Objective: To study the permeability of leflunomide and the effect of liposome on its permeability. Methods: Modified Franz diffusion cell adopted as apparatus for in vitro skin permeation. Various excised skins were used as permeation barriers and permeation coefficient was used as index. The concentration of leflunomide in the samples was measured by HPLC. Results: Leflunomide had a good permeability, but the effect of liposome on permeability was not very strong. Conclusion: It is feasible that leflunomide is applied through skin. [

Merely having complete sequences of genomes is not sufficient to elucidate biological function. Proteomics is complementary to genomics because it focuses on the large scared gene products, which are the active agents in cells. As almost all the drugs are directed against proteins, proteomics have been widely used in pharmacological research.Proteomics profiling in drug target identification, toxicological and drug resistances research was reviewed.

Transcutaneous immunization(TCI),the topical application of antigen and adjuvant directly onto intact skin,which can safely and effectively elicit systemic and celluar immune responses in mice and humans against a variety of antigens,is a novel method of vaccine delivery.Not only the skin has evolved as a barrier to prevent external agents,including pathogens,from entering the body,but also it has a complex and efficient immune system,which includes Langerhans′ cells and dendritic cells.The skin is an attractive target for vaccine delivery.By targeting the body′s natural defense system,TCI attempts to produce an efficient immune response.All these results highlight the likelihood that TCI will play an important role in vaccine applications in the future.

Aim To explore the expression of TNF-related apoptosis-inducing ligand(TRAIL)between psoriatic patients and healthy subjects.Methods Immunohistochemical staining and Real-Time PCR methods were established to detect the expression of TRAIL.Results Immunohistochemical staining results indicated a significant difference of the expression of TRAIL between psoriatic patients and healthy subjects.Real-Time PCR results indicated that the quantities were 0.42?0.07,1.01?0.16 respectively of two type skins.Thus there were significant differences(P

Objective:To explore the effects of clinical pharmacist on the rational drug use in the patients with membranous ne -phropathy complicated with drug-induced liver injury .Methods:The whole drug treatment process of one patient with membranous ne-phropathy complicated with drug-induced liver injury was analyzed , pharmaceutical care on the use of glucocorticoids , hepatoprotective agents, anticoagulants and antihypertensive drugs was performed , and the precautions and suggestions on the drug use were put for-ward.Results:Clinical pharmacist participated in the clinical treatment and provided the pharmaceutical service for doctors , nurses and patients directly , which could enhance the therapeutic efficacy and reduce the adverse drug reaction .Conclusion: Through the participation in clinical practice , clinical pharmacist plays an important role in the rational drug use , which provides better pharmacy service for patients .

Objective:To explore the therapeutic regimen and key points in the pharmaceutical care for the elderly patients with a-cute exacerbation of the chronic obstructive pulmonary disease ( AECOPD) . Methods: On the basis of clinical pharmacist work, one typical case was selected. Referring to the COPD treatment guidelines, the treatment rationality was analyzed. Meanwhile, an individu-alized pharmaceutical care plan was established and carried out in whole process of the treatment. Results:The pharmacotherapy was effective and rational. By providing the pharmaceutical care for the AECOPD patient, the related problems in the treatment were solved promptly, and the rational advice on the drug treatment was provided. Conclusion:It is very important to enhance integrated pharma-ceutical care in the elderly patients with AECOPD.

This study is to prepare scopolamine hydrobromide nanoparticles-in-microsphere system (SH-NiMS) and evaluate its drug release characteristics in vitro. SH nanoparticles were prepared by ionic crosslinking method with tripolyphosphate (TPP) as crosslinker and chitosan as carrier. Orthogonal design was used to optimize the formulation of SH nanoparticles, which took the property of encapsulation efficiency and drug loading as evaluation parameters. With HPMC as carrier, adjusted the parameters of spray drying technique and sprayed the SH nanoparticles in microspheres encaposulated by HPMC was formed and which is called nanoparticles-in-microsphere system (NiMS). SH-NiMS appearances were observed by SEM, structure was obsearved by FT-IR and the release characteristics in vitro were evaluated. The optimized formulation of SH nanoparticles was TPP/CS 1:3 (w/w), HPMC 0.3%, SH 0.2%. The solution peristaltic speed of the spray drying technique was adjusted to 15%, and the temperature of inlet was 110 degrees C. The encapsulation product yeild, drug loading and particle sizes of SH-NiMS were 94.2%, 20.4%, and 1256.5 nm, respectively. The appearances and the structure of SH-NiMS were good. The preparation method of SH-NiMS is stable and reliable to use, which provide a new way to develop new dosage form.