Merely having complete sequences of genomes is not sufficient to elucidate biological function. Proteomics is complementary to genomics because it focuses on the large scared gene products, which are the active agents in cells. As almost all the drugs are directed against proteins, proteomics have been widely used in pharmacological research.Proteomics profiling in drug target identification, toxicological and drug resistances research was reviewed.

Objective To study the clinical and imaging features of paraneoplastic limbic encephalitis (PLE). Methods The clinical, latoratorial and imaging information of two patients with clinically diagnosed PLE associated with invasive thymoma or lung cancer are reported. Results Two cases had typical findings in clinical symptoms ( severe neuropsychological deficits, disorientation, memory impairment and seizures) and MRI showed ( abnormal high-signal intensity in hippocampal formations and temporal lobe on T2-weighted imagings) , One patient was investigated by cerebral 18 F-fluoro-2-deoxy-D- glucose- positron emission tomography (FDG-PET) , and highly significant focal hypermetabolism in bilateral temporal and other areas was found. Conclusion The clinical and imaging examination are the improtant method for the diagnosis of PLE.

Objective:To explore the effects of clinical pharmacist on the rational drug use in the patients with membranous ne -phropathy complicated with drug-induced liver injury .Methods:The whole drug treatment process of one patient with membranous ne-phropathy complicated with drug-induced liver injury was analyzed , pharmaceutical care on the use of glucocorticoids , hepatoprotective agents, anticoagulants and antihypertensive drugs was performed , and the precautions and suggestions on the drug use were put for-ward.Results:Clinical pharmacist participated in the clinical treatment and provided the pharmaceutical service for doctors , nurses and patients directly , which could enhance the therapeutic efficacy and reduce the adverse drug reaction .Conclusion: Through the participation in clinical practice , clinical pharmacist plays an important role in the rational drug use , which provides better pharmacy service for patients .

Objective:To study the wound healing effects of the extracts of Shengji Huayu Fang with different solvents and demon-strate their important role in wound healing for deep second degree burn wound. Methods:Sixty Sprague-Dawley rats were randomly di-vidied into six groups(ten rats in each group): the control group (saline), the positive control group (asiaticoside group), Shengji Huayu Fang group respectively with petroleum ether, chloroform, ethyl acetate and n-butanol as the extraction solvent. The extracts of Shengji Huayu Fang with different solvents were applied in the rats with deep second degree burn wound, and the wound healing was e-valuated by the healing rate judged by naked eyes and computer image analysis, DNA cell cycle analysis using a flow cytometry, patho-logical reports and the degree of re-epithelialization studied by the other methods. Results:The mean healing time of the chloroform ex-tracts group [(15. 67 ± 1. 12)d] was much shorter than that of the control group [(22. 87 ± 1. 01)d, P<0. 01]. The hydroxyproline content and the percentage of S-phase cells in wound tissue in the chloroform extracts group was obviously higher than those in the con-trol group (P<0. 01). Conclusion:The present study indicates that topical application of chloroform extracts of Shengji Huayu Fang is beneficial to burn wound healing, and the chloroform extracts of Shengji Huayu Fang is the main bioactive fraction.

Objective:To explore the therapeutic regimen and key points in the pharmaceutical care for the elderly patients with a-cute exacerbation of the chronic obstructive pulmonary disease ( AECOPD) . Methods: On the basis of clinical pharmacist work, one typical case was selected. Referring to the COPD treatment guidelines, the treatment rationality was analyzed. Meanwhile, an individu-alized pharmaceutical care plan was established and carried out in whole process of the treatment. Results:The pharmacotherapy was effective and rational. By providing the pharmaceutical care for the AECOPD patient, the related problems in the treatment were solved promptly, and the rational advice on the drug treatment was provided. Conclusion:It is very important to enhance integrated pharma-ceutical care in the elderly patients with AECOPD.

Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory demyelinating disease of the central nervous system, primarily affecting the optic nerve and spinal cord.In addition to aquaporin 4 antibody, more and more studies have shown that interleukins (IL) also play an important role in the progression of NMOSD.For example, among the most common CD4 + T cells, Th17 cells can destroy the blood-brain barrier to invade the central nervous system and then affect autoimmunity; Th2-mediated IL-4 and IL-10 regulate immune activities by activating macrophages; Tregs can suppress the immune response by restricting various immune cell functions.The levels of related IL in the cerebrospinal fluid and blood of NMOSD patients vary at different stages of the disease course.This article mainly reviews the role and a change characteristics of interleukins related to NMOSD, which helps us to understand its pathogenic mechanism in-depth, and discusses whether IL can be used as immunophysiological markers to diagnose NMOSD.

Objective:To establish the quality standard for Qinzhu Liangxue mixture. Methods:Seutellaria baiealensis, glyeyrrhizae and jobstears seed were identified by TLC. Baicalin and glycyrrhizic acid were determined by HPLC. Results:Seutellaria baiealensis,glyeyrrhizae and jobstears seed could be identified by TLC. The linear range of baicalin was 0. 030- 0. 971 mg·ml -1(r = 1. 000 0),and the average recovery was 97. 74%(RSD = 2. 76% ,n = 9). The linear range of glycyrrhizic acid was 0. 013- 0. 220 mg·ml -1(r = 1. 000 0),and the average recovery was 99. 02%(RSD = 1. 79% ,n = 9)Glycyrrhizic acid. Conclusion:The method is specific,simple and accurate. It can be used for the quality control of Qinzhu Liangxue mixture.

Objective To assess the diagnosis value of diffusion- weighted imaging (DWI) in Creutzfeldt-Jakob disease (CJD).Methods 8 cases of sporadic CJD who underwent MRI were reported. 4 cases were definite,3 cases were probable and 1 case was possible. The sensitivity of DWI and conventional MRI were compared. Results T_1WI and T_2WI revealed no abnormal signals except nonspecific diffuse brain atrophy in 4 cases, whereas DWI detected hyperintense abnormalities in all cases. 2 cases showed linear lesions only in the cerebral cortex, and 6 cases showed lesions in both the cerebral cortex and the striatum. The lesions were symmetric in 5 cases, but were asymmetric in the other 3 cases. Although fluid-attenuated inversion recovery (FLAIR) imaging also showed cortical hyperintensity in 1 case, the high signal changes were more evident and extensive on DWI. Conclusions The hyperintense changes in the cerebral cortices and/or striata on DWI are considered characteristic of CJD. DWI is more sensitive than conventional MRI in depicting lesions of CJD and may be an essential tool for the early diagnosis of this disease.

Objective To inquire into the location of the relevant gene mutations in the Chinese familial hypokalaemic periodic paralysis, and to specify the correlation between the genotype and the clinical features of this disease. Methods Target-exon PCR and DNA direct sequencing were used to research the mutations in the CACNA1S, SCN4A, and KCNE3 genes of 14 familial hypokalaemic periodic paralysis probands. If a positive member was found, the other members of his (her) family must be inspected with the sequencing method. Results The probands of 3 families showed the known correlating mutations of hypokalaemic periodic paralysis, which were R1239H mutations in the CACNA1S in 1 family and R672H mutations in the SCN4A in the other 2 families. In addition, the differences of the age of onset, the responsibility to the treatment with acetazolamide and penetrance were found between the CACNA1S R1239H and SCN4A R672H mutations. Conclusions SCN4A R672H and CACNA1S R1239H mutations exist in the Chinese familial hypokalaemic periodic paralysis. Differences of the clinical features exist, resulting from these 2 kinds of mutations.

Objective:To review and analyze the prescriptions for Chinese herbal pieces in an integrative traditional Chinese and western medicine hospital to explore the trend and problems in clinical application for Chinese herbal medicines and provide reference for dispensing in pharmacy of traditional medicine and rational drug use. Methods: Totally 1200 prescriptions for Chinese herbal pieces in our hospital were surveyed from January to December in 2014. The irrational use was analyzed, and reasonable suggestions were provided for the clinical application. Results:The problems in the prescriptions for Chinese herbal pieces included uncompleted diagnosis,incompatible diagnosis and medication,nonstandard Chinese name,unclear footnote,irrational usage or dosage and incompati-bility. The prescriptions including above 15 varieties of Chinese herbal pieces in one accounted for 65. 70%, and those with average dose above 250 g accounted for 40. 25%. Conclusion:Regular inspection and quality supervision on the prescription for Chinese herb-al pieces should be strengthened to improve the quality of prescriptions and guarantee the safety and economy of the medication.