Objective To evaluate the effect of furosemide on Cl -/HCO- 3 exchange of inner medullary collecting duct(IMCD) in rabbit kidney. Methods The effect of furosemide in different concentrations on the changes in Cl -/HCO- 3 exchange in mono-layer of IMCD cell in rabbit kidney was determined by fluorescent probe technique. Results Cl -/HCO- 3 exchange in IMCD cell could be inhibited by 4.3% by 15?mol/L furo semide solution, and 480?mol/L furosemide solution could inhibit the exchange by 97.4%. The Cl -/HCO- 3 exchange rates of the groups, in which the final concentrations of furosemide were equal to or higher than 30?mol/L, were significantly lower than that of the control group(P

The immunoregulatory effect of TLSF_(JM) on the levels of IP3,Ca~(2+) in activated T cells andthe expression of Fos protein was investigated by ABC immunohistochemistry technique and Fu-ra-2/AM labelled T cells and anion-exchange chromatography techniques in this paper.The re-sults showed that TLSF_(JM) can the same time,it can strongly inhibit the levels of IP3、Ca~(2+) in ac-tivated T cells.

Adult ; Aged ; Arthritis, Rheumatoid ; complications ; Female ; Humans ; Intracranial Thrombosis ; complications ; Male ; Middle Aged ; Pulmonary Embolism ; complications ; Venous Thrombosis ; complications

AIM:To study the isolation and identification of curdione from Zedoary oil. METHODS: Silica column was adopted to isolate constituents from Zedoary oil;IR,GC/MS and NMR methods were used to identify its structure. RESULTS: Three constituents were isolated from Zedoary oil,including crystal C_1,C_2 and C_3.Crystal C_1 was identified to be curdione and curdione content in Zedoary oil was 11.31%. CONCLUSION: This method is simple and convenient and it can be used to isolate more quantity of curdione for further pharmaceutical study.

Objective To establish a HPLC fingerprint analysis method of salt-prepared Cortex Phellodendri. Methods C18 column was used, with gradient elution by the mobile phase consisted of acetonitrile-0.1 % phosphoric acid (contained 0.2 % triethylamine). The detection wavelength was at 230 nm, and the flow rate was at 0.8 mL/min. Results Seventeen characteristic peaks were selected and the fingerprints on HPLC was set up. Conclusion HPLC fingerprint method is reproducible, accurate and stable, and can be used for the quality control of salt-prepared Cortex Phellodendri.

Esophagus ; Foreign Bodies ; Humans ; Infant ; Male

The Medical Healthcare Service Center,located at the Youxian District,Mianyang City of Sichuan Province,successfully rescued the people affected by the Wenchuan earthquake and the Tangjiashan barrier lake with great help from the senior hospitals.We suggest that the community healthcare service center could play an important role in emergency response system.

Bacillus cereus B-04 antagonist to Botrytis cinerea were isolated from samples of tomato soil infected by Botrytis cinerea in Zibo, which are identified through a series of morphological and biochemical characteristics and the sequence of 16SrDNA. Aiming at enhancing the inhibitory effect of this strain, a 4.1kb DNA fragment containing ?-1,3-glucanases gene from pUC1940 was inserted into vector pBE2 and pHY300PLK to construct recombination plasmids, PBE2-glu and pHY300PLK-glu, which were transferred into Bacillus cereus B-04, resulting in a new strain named B-04-glu. Restriction enzyme digestion and ?-1,3-glucanases plate culture confirmed that B-04-glu contained a functional ?-1,3-glucanases gene. Compared to the wild strain B-04, B-04-glu had an increased inhibitory effect against Botrytis cinerea on tomato.

Objective To study the expression of caspase-3,survivin,CyclinD1 and P27 in gastric carcinoma(CA2)and the relationship between the biomarkers and clinical pathological parameters. Methods Immunohistochemical Ready-to-use two-steps method was performed to research the expression of the four proteins in 72 cases of GC,and 54 cases of normal gastric mucosa.Results The expression level of caspase- 3 and p27 protein in GC was significantly lower than that in normal gastric mucosa(P

To observe the curative effect of treating small -incision cataract extraction by intraocular lens implantation combined with trabeculectomy for primary angle-closure glaucoma with cataract.
●METHODS: Totally 44 cases (52 eyes) of primary angle-closure glaucoma combined with cataract were selected to undergo the combined surgery, in order to observe the patients' pre - and postoperative eyesights, intraocular pressures and the postoperative complications.
●RESULTS: The postoperative eyesight was improved significantly as compared with the preoperative eyesight. The intraocular pressure was declined dramatically. The result was of statistical significance (P<0. 05). All the 52 cases' surgeries were performed by the same surgeon. The surgeries were processed smoothly, with 6 postoperative eyes of anterior chamber inflammation cell response, 3 eyes of anterior chamber fibrinoid exudate, 2 eyes of shallow anterior chamber through mydriasis and treatment with glucocorticoids and non - steroidal eyedrops before absorption, and no complications like malignant glaucoma, cyclodialysis, etc. were reported through mydriasis and pressure bandaging before recovery.
● CONCLUSlON: Treating the primary angle - closure glaucoma combined with cataract through the combined surgery has high reliability and desirable curative effect. The surgical method is simple to learn and applicable for promotion on the basic level.