Communicable Diseases, Emerging ; microbiology ; Humans

Adamantane ; therapeutic use ; Amantadine ; therapeutic use ; Antiviral Agents ; therapeutic use ; Child ; Guanidines ; Humans ; Influenza, Human ; drug therapy ; prevention & control ; Pyrans ; Rimantadine ; therapeutic use ; Sialic Acids ; therapeutic use ; Zanamivir

Bronchiolitis ; epidemiology ; pathology ; therapy ; Bronchiolitis Obliterans ; epidemiology ; pathology ; therapy ; Child ; Haemophilus Infections ; epidemiology ; pathology ; therapy ; Humans

Child ; Chronic Disease ; Cough ; diagnosis ; Humans

The irrational drug use is one of the major public health issues. As children are in growth, their drug absorption, distribution, metabolism, excretion and pharmacodynamic characteristics are different from those in adults, thus more challenges on how to make rational use of drugs in children are seen. In this paper, by analyzing current situation of irrational drug use in children, the definition and standards of the rational drug use, and the measures adopted by WHO and China in promoting rational use of drugs in children, we emphasize here and call for a promotion of the awareness of rational use of drugs in children, and activities in forms of propaganda and health education, popularization of medical science and the medication safety knowledge, guidance for the development of good medication beliefs and habits in public, improvement of the social security administration, maximization of the protection of children's medication safety, and maintenance of children's health rights and interests.

Child ; Humans ; Mycoplasma pneumoniae ; Pneumonia, Mycoplasma

Child ; Humans ; Pneumonia, Pneumococcal ; Streptococcus pneumoniae

Anti-Bacterial Agents ; pharmacology ; Child ; Drug Resistance, Bacterial ; genetics ; Humans ; Macrolides ; pharmacology ; Pediatrics ; Streptococcus pyogenes ; drug effects

Objective: To investigate the influence of TTS-12, a steroid saponin from Tribulus terrestris L., on the formation of Candida albicans biofilm, and to discuss the possible mechanism. Methods: The inhibition of C. albicans biofilm formation by TTS -12 was observed by confocal scanning laser microscopy; XTT method was used to investigate the influence of different concentrations of TTS-12 on C. albicans biofilm formation. The water-hydrocarbon two-phase assay was used to measure the cell surface hydrophobicity of C. albicans treated with different concentrations of TTS-12. The expression of CSH1 mRNA was measured by real-time RT-PCR. Results: Compared with control group, TTS-12 treatment resulted in loose C. albicans biofilm, and it dose-dependently inhibited C. albicans biofilm formation. The cell surface hydrophobicity in the TTS-12-treated groups was lower than that in the control group; consistent with this, TTS-12-treated cells also expressed significantly lower levels of CSH1 mRNA than cells in control group (P<0. 01). Conclusion: TTS-12 may inhibit the formation of C. albicans biofilm through inhibiting CSHI gene expression.