In the early stage of tumor,the diagnosis index of objective and definite is often deficient,which brings great trouble to the treatment.How to find some valuable markers or characteristic changes in the early stage of cancer is the key problem to be solved by the medical experts.With the development of flow cytometry (FCM),it is playing a more and more important role in precancerous lesion screening,early detection of cancer,chemotherapy guidance and prognosis assessment.In personalized medicine and precise medical background,the application of FCM in tumor marker detection,cancer stem cells separation screening,isolation and identification of circulating tumor cells is especially worth looking forward tc.

Breast reconstruction after radical mastectomy significantly improves the patient' s quality of life and has become an important part of comprehensive treatments. However, there are a number of reconstruction problems. The choice of breast reconstruction after breast cancer should be considered in general. Immediate reconstruction is not superior to breast-conserving surgery, while immediate reconstruction is superior to delayed reconstruction, and choices vary between implant reconstruction and autologous reconstruction. In short, breast reconstruction should be based on the radical principle with balance of beauty.

Carcinoma, Squamous Cell ; genetics ; Cell Division ; Chromosome Deletion ; Humans ; Laryngeal Neoplasms ; genetics ; Telomere ; genetics ; Tumor Cells, Cultured

Objective To investigate the comparability of K + ,Na+ and Cl- measurement between Cobas8000 analyzer and AU640 analyzer .Methods According to the EP9‐A2 document of the National Committee for Clinical Laboratory Standards ,the precision of AU640 analyzer for the detection of K + ,Na+ and Cl- was evaluated ,and the correlation between the detected results of the two analyzers were also analyzed .Results AU640 analyzer were with high precision for the detection of K + ,Na+ and Cl- ,in‐tra‐assay and inter‐assay coefficient of variation of which were both less than 2% .The correlation coefficients of the two analyzers were higher than 0 .975 .Conclusion Cobas8000 analyzer and AU640 analyzer might be with fine comparability for the detection of K+ 、Na+ and Cl- ,both of which could provide accurate detected results .

Objective To explore the pattern of cognitive impairments mainly caused by the white matter lesions(WML) in the frontal lobe in patients with subcortical ischemic vascular disease (SIVD).Methods Fifty SIVD patients were divided into severe WML group (visual score >3, n=27) and mild WML group(visual score ≤3, n=23) according to their severities of the WML in frontal lobe .Seven patients without SIVD were collected as controls .All patients underwent a set of neuropsychological battery ,and the results were analyzed .Results There was no statistical significance among three groups on basic data .Compared with mild WML group and control group , non frontal white matter scores and numbers of lacunes in frontal lobe of severe WML group were significantly higher ( all P=0.000).Compared with mild WML group,the Montreal cognitive assessment scale in severe WML group were significantly lower ( P=0.047 ) , and scores related to the executive function were significantly lower ( P=0.006 ) , even after adjusting the numbers of lacunes in frontal lobe ,there was statistically significant difference (P=0.038). Multiple regression confirmed that the Z scores of executive functions were mainly affected by white matter lesions located in the frontal lobe ( P=0.000 ) .Conclusion WML located in the frontal lobe mainly affect the executive function in patients with SIVD .

Adult ; Brain Hemorrhage, Traumatic ; diagnosis ; etiology ; Humans ; Male ; Neck Injuries ; complications ; diagnosis ; Vertebral Artery

Bacterial Infections ; microbiology ; therapy ; Humans ; Integrative Medicine ; Male ; Microbial Sensitivity Tests ; Middle Aged ; beta-Lactamases ; isolation & purification

AIM　To investigate the chemical constituents of the rhizomes of Dioscorea panthaica Prain et Burkill and look for new active compounds. METHODS　The compounds were isolated with silica gel and HPLC, and their structures were elucidated on the basis of chemical evidences and spectral analysis (IR, ESI-MS, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, HMQC, HMBC, NOESY). RESULTS　Three steroidal saponins were isolated from the rhizomes of Dioscorea panthaica. They were elucidated as 26-O-β-D-glucopyranosyl-3β,26-diol-23(S)-methoxy-25(R)-Δ5,20(22)-diene-furosta-3-O-［α-L-rhamnopyranosyl-(1→2)-O-α-L-rhamnopyranosyl-(1→4)］-β-D-glucopyranoside (I), pseudoprotodioscin (II), 26-O-β-D-glucopyranosyl-3β,26-diol-25(R)-Δ5,20(22)-diene-furosta-3-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-glucopyranoside (III). CONCLUSION　Compound I is a new steroidal saponin and was named as dioscoreside C. Compound II and III were obtained from this genus for the first time.

Objective To study CT and MRI features and classification of brain schistosomiasis granuloma. Methods CT and MRI data of 30 cases of brain schistosomiasis granuloma were reviewed.All cases were proved by the surgery or pathological examination and clinical laboratory test.There were 20 males and 10 females, and their age ranged from 5 to 58 years, mean 29.2 years.Plain and enhanced CT were performed in all patients with GE MAX 640 scanner.Ten patients were examined by plain and enhanced MRI with GE Signa profile 0.2 Tesla open scanner.Results The lesions located in supratentorial region in 27 cases and in infratentorial region in 3cases.The nodules were isodense or slight hyperdense on CT plain scan, iso or hypointense on T_1WI, hyperintense on T_2WI, Slight hyperintense on FLAIR.After the contrast material was injected intravenously, CT and MRI findings were multiple or single enhanced nodules at the cortical or subcortical area.There were four types of imaging features: (1) multiple small nodules in 5 cases (presenting as bright stars in the dark sky); (2) single large nodule in 8 cases; (3) mixed nodules in 14 cases; (4) circle-enhanced nodules in 3 cases.Conclusion The brain schistosomiasis granuloma has typical CT and MRI findings.CT and MRI classification is not only helpful to its diagnosis and differential diagnosis, but also might be useful for the choice of clinical treatment.

Objective:To investigate the blood lipid status and its variation regularity of a population of teaching staff in order to provide evidence for preventing hyperlipidemia.Method:Lipid indexes such as TC,Triglyceride(TG),LDL-C and HDL-C are inspected of a group of teaching staff over 40 years old in some certain university,and data statistics and analysis are conducted.Conclusion:Main behaviors of male and female teaching staff's hyperlipidemia are high TG and high LDL-C.Special focus should be put on blood lipid's variation level of middle aged males around 40 years old,periodical clinical monitoring should be done for female teaching staff over 50 years old.Medical information instruction on the prevention and control of high blood lipid should be strengthened in order to realize the unity of self control and social control.